High resolution Adama conversion micro-image analyzer
A technology of Hadamard transformation and microscopic image, applied in high sensitivity, high signal-to-noise ratio, can solve the problem of low resolution, achieve high resolution, avoid measurement error, and solve the effect of mechanical modulation accuracy
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Embodiment 1
[0030] Embodiment 1, Hadamard transform microspectral image analyzer.
[0031] See figure 1 , figure 2 . The analysis sample is placed on the research-type epi-fluorescence microscope stage produced by Chongqing Optical Instrument Factory, and the image signal (transmitted light or fluorescence) of the analysis sample is collected by the microscope objective lens, and the Hadamard transform optical system on the fluorescence microscope The component total reflection mirror P is pushed to the optical path, and the image of the field of view is turned 90 degrees, and then enters the image composed of converging lens group L1, mirror M1, Hadamard transformation template I and diaphragm, collimating lens group L2, grating G, mirror M1, The cylindrical lens L3 passes through the slit S, namely the Hadamard transform microspectral image analyzer as shown in the figure. The lens group L1 compresses the image of the microscopic field of view, and the image is clearly imaged on the...
Embodiment 2
[0033] Example 2: Quantitative analysis of fluorescent images of human breast cancer cells, morphology of nuclei and DNA content.
[0034] Take direct smears of breast tumor puncture fluid, use normal human peripheral venous blood lymphocytes or normal epithelial cells in breast tumor specimens as standard diploid cells, and use acridine orange (AO, 50 μg / mL, containing 1% Triton X- 100) is a cellular DNA fluorescent probe, and the DNA content (ploidy) of human breast tumor cells is measured with a Hadamard transform microspectral image analyzer. At least 20 tumor site cells are analyzed for each patient, and two quantifications are measured for each cell. Data: Nuclei DNA content (in arbitrary units) and morphology (measured area in pixels). The measurement data shows that the Hadamard transform spectral image can correctly reflect the basic fluorescence distribution of cells, and obtain quantitative fluorescence intensity and distribution information. Compared with standard...
Embodiment 3
[0035] Example 3: Preliminary screening of anticancer drugs for cell cycle non-specific drugs.
[0036] Using human peripheral venous blood lymphocytes or mouse hepatocytes as materials, using fluorescent probe AO (acridine orange) to trace to determine whether the drug can enter the nucleus and its ability to bind to nuclear DNA, a method targeting nuclear DNA is proposed. A new method for preliminary screening of anticancer drugs based on cell cycle non-specific drugs. The specific method is to label human peripheral venous blood lymphocytes or mouse liver cells with fluorescent probe AO, and use Hadamard transform microscopic fluorescence image analyzer to detect single cells. The fluorescence intensity of the AO-DNA complex in the nucleus at emission peak 530nm. The fluorescence inhibition rate of the drug on the cellular DNA-AO complex ((F 0 -F) / F 0 ×100%), that is, the strength of the interaction between the drug and the cell DNA, is used as the basis for the prelimina...
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