Microbial fodder additive and its preparing method and use

A microbial feed and additive technology, applied in animal feed, animal feed, applications, etc., can solve the problems of product contamination by bacteria, unstable efficacy, and low content of viable bacteria

Active Publication Date: 2006-10-25
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to use three types of 4 strains of beneficial functional microorganisms including lactobacillus, saccharomyces and bacillus to rationally compound compatibility, adopt liquid submerged fermentation technology and a series of post-processing technology to overcome the existing microbial feed additives It has the defects of single function, low content of viable bacteria in the product, short survival time of viable bacteria cells, serious contamination of the product by bacteria, unsatisfactory use effect, and unstable efficacy. Microbial feed additive with less pollution, which can improve feed nutrient content and feed utilization

Method used

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  • Microbial fodder additive and its preparing method and use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Embodiment 1, cultivate the fermented liquid (A) of bacillus subtilis

[0088] 1) plate culture rejuvenation: the preserved strains of Bacillus subtilis were streaked and inoculated on the Bacillus plate medium by streak inoculation, and cultured at 30°C for 18 hours to rejuvenate the Bacillus subtilis and form a single colony; Described bacillus subtilis culture medium is the mixture prepared in the following proportions: peptone 0.5% (W / V, the same below), glucose 0.5%, beef extract 0.5%, potassium dihydrogen phosphate 0.3%, agar 3%, pH= 7.5~7.6, sterilized at 121°C for 30 minutes, set aside;

[0089] 2) Make shake flask (triangle flask) seeds: select a typical single colony from the Bacillus subtilis colony cultivated in step 1), inoculate it into a Bacillus liquid medium, and cultivate it at 32°C for 30 hours to obtain a Bacillus subtilis shake flask Seed liquid; the bacillus liquid medium is a mixed solution prepared in the following proportions: 0.5% peptone, 2% gl...

Embodiment 2

[0092] Embodiment 2, cultivate the fermented liquid (A) of bacillus subtilis

[0093] 1) plate culture rejuvenation: the preserved strains of Bacillus subtilis were streaked and inoculated on the Bacillus plate medium by streak inoculation, and cultured at 30°C for 18 hours to rejuvenate the Bacillus subtilis and form a single colony; Described bacillus subtilis culture medium is the mixture prepared in the following ratio: peptone 0.5% (% refers to weight volume percentage, the same below), glucose 0.5%, beef extract 0.5%, potassium dihydrogen phosphate 0.3%, agar 3%, pH=7.5~7.6, sterilized at 121°C for 30 minutes, and set aside;

[0094] 2) Make shake flask (triangle flask) seeds: select a typical single colony from the Bacillus subtilis colony cultivated in step 1), inoculate it into a Bacillus liquid medium, and cultivate it at 32°C for 30 hours to obtain a Bacillus subtilis shake flask Seed liquid; the bacillus liquid medium is a mixed solution prepared in the following ...

Embodiment 3

[0098] Embodiment 3, cultivate the fermented liquid (B) of Bacillus cereus

[0099] Microbial kind is bacillus cereus (Bacillus cereus), and substratum, condition, preparation method are with embodiment 1, obtain bacillus cereus fermented liquid, finally according to bacillus cereus plate culture rejuvenation used substratum and culture condition, to The live bacteria content of the Bacillus cereus fermentation broth was detected by conventional plate counting. According to the counting results, the live bacteria content was diluted to 10% by sterilized tap water. 8 CFU / ml, 10 9 CFU / ml and 10 10 CFU / ml, to obtain the standby solution (B) of the Bacillus cereus fermentation broth, to be post-processed.

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Abstract

The present invention relates to microbial fodder additive, and a kind of microbial fodder additive comprising beneficial microbes and their metabolites and its preparation process and use. The microbial fodder additive is the mixture of fermented liquid or dry powder obtained with Bacillus subtilis, Bacillus cereus, lactobacillus and Saccharomyices cerevisiae and through high density deep layer liquid fermentation. It may be prepared into different specific liquid or powder preparations. The microbial fodder additive has high live bacillus content, long product preserving period and less foreign bacteria contamination, and has the functions of raise fodder utilization, promote animal's growth, increase animal product yield and quality, etc.

Description

technical field [0001] The invention relates to a microbial feed additive, in particular to a microbial feed additive composed of various beneficial microorganisms and their metabolites (such as protease, lipase, amylase, cellulase, etc.) and its preparation method and use. Background technique [0002] The use of some growth-promoting health care agents in feed industry and breeding industry has promoted the rapid development of animal husbandry, but at the same time it has brought many potential threats to human health and the development of feed industry. At present, the abuse of antibiotics, chemical synthetic drugs, hormones, β-stimulants, heavy metals, sedatives and other growth-promoting health care agents in feed and livestock breeding has led to a series of problems and consequences: (1) leading to serious drug residues in livestock and poultry products, directly It is harmful to human health, and even produces "three diseases", and makes my country's livestock and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16C12N1/20A23K10/18
Inventor 张日俊
Owner CHINA AGRI UNIV
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