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Preparation method of earthworm kinase dry powder

A technology of lumbrokinase dry powder and lumbrokinase, which is applied to biochemical equipment and methods, medical preparations containing active ingredients, enzymes, etc., can solve the problems of low content of active ingredients and many impurities in dry earthworm powder, and achieve easy industrial Amplified, high-purity effects

Active Publication Date: 2006-10-25
BEIJING BAIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the dry earthworm powder prepared by this method has low content of active components and more impurities, which may easily cause some side effects that are inconsistent with the purpose of treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Earthworm cleaning: After washing 10 kg of live earthworms with water, add 0.2 kg of cotton white sugar per kg of earthworms, stir evenly at room temperature, let stand for 40 minutes, stimulate the earthworms to spit out the secretions in their digestive tract, and then use 30 The warm water at ℃ washes away the sugar and dirt on the earthworms;

[0027] 2) pulping: adding water of 1 times the volume of earthworms, using a high-pressure homogenizer to crush the earthworms cleaned in step 1) for 30 minutes to make a homogenate;

[0028] 3) slag removal: the homogenate obtained in step 2) is filtered through a stainless steel filter with a screen aperture of 5 mm to remove the coarse slag in the homogenate;

[0029] 4) clarification: after step 3) the homogenate liquid after deslagging is processed by a high-speed centrifuge for 2 hours to obtain a clarified liquid;

[0030] 5) Separation: using DEAE-ion exchange chromatography column to separate the earthworm clarif...

Embodiment 2

[0036] 1) Earthworm cleaning: After washing 10 kg of live earthworms with water, add sodium chloride at a ratio of 0.3 kg of sodium chloride per kg of earthworms, stir evenly at room temperature and let stand for 40 minutes to stimulate the earthworms to release the secretions in their digestive tract Spit it out, and then wash away the sodium chloride and dirt on the earthworms with warm water at 40°C;

[0037] 2) Pulping: add water 8 times the volume of the earthworm, and use a bead mill to crush the earthworms cleaned in step 1) to make a homogenate;

[0038] 3) slag removal: the homogenate obtained in step 2) is removed with a centrifuge to remove the coarse slag in the homogenate;

[0039] 4) clarification: after step 3) the homogenate liquid after deslagging is processed by a high-speed centrifuge for 2 hours to obtain a clarified liquid;

[0040] 5) Separation: using DEAE-ion exchange chromatography column to separate the earthworm clarified liquid obtained in step 4),...

Embodiment 3

[0046] 1) Cleaning of earthworms: After washing 10 kg of live earthworms with water, add 0.3 kg of granulated sugar per kg of earthworms, stir evenly at room temperature, let stand for 20 minutes, stimulate the earthworms to spit out the secretions in their digestive tract, and then use 50 ℃ The warm water washes away the sugar and dirt on the earthworms;

[0047] 2) pulping: adding water 6 times the volume of earthworms, using a colloid mill to crush the earthworms cleaned in step 1) for 30 minutes to make a homogenate;

[0048] 3) slag removal: the homogenate obtained in step 2) is filtered through a stainless steel filter with a screen aperture of 20 mm to remove the coarse slag in the homogenate;

[0049] 4) clarification: after step 3) the homogenate liquid after deslagging is treated with a microporous membrane filter for 2 hours to obtain a clarified liquid;

[0050] 5) Separation: Use DEAE Affi Gel Blue triazine dye affinity chromatography column to separate the earthwo...

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Abstract

The invention relates to a preparation method of lumbrokinase dry powder. The method comprises the steps of: washing live earthworms with water, placing them in sugar or sodium chloride, standing at room temperature, and stimulating the earthworms to vomit the secretions in their digestive tracts; Then add water 1 to 8 times the volume of earthworms to break the cells, and after the homogenate is removed and clarified, it is separated by anion exchange chromatography column or affinity chromatography column, and the active peak part is collected. The effluent is concentrated and desalted, then filtered and sterilized; the obtained clarified liquid is frozen at -30~-40°C; then dried under vacuum to obtain lumbrokinase dry powder, or the clarified liquid is sprayed under sterile conditions In the dryer, it is atomized by compressed air and then dehydrated to obtain dry powder of lumbrokinase. The lumbrokinase dry powder obtained by the method has high effective component content, easy quality control, and easy large-scale industrial production.

Description

technical field [0001] The invention relates to a preparation method of lumbrokinase dry powder. technical background [0002] Lumbrokinase dry powder is a kind of biopharmaceutical made from live earthworms, which is light yellow powder. This drug is a multi-component enzyme preparation, including plasmin and plasminogen activator, which can directly dissolve fibrin and activate zymogen to indirectly dissolve protein. It is suitable for treating heart and cerebrovascular embolism diseases and preventing diseases with increased fibrinogen and increased platelet aggregation rate. Clinical trials have proved that the capsules of this drug have remarkable recovery effects on ischemic cerebrovascular disease, stroke paralyzed limbs and language barriers, and are a safe fibrinolytic drug. After treatment, the patient's fibrinogen significantly decreased to the normal level, the euglobulin dissolution time was significantly shortened, the whole blood viscosity was significantly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/64A61K38/17A61P9/14A61P7/02A61P9/10A61K38/48C12N9/68
Inventor 万兴中
Owner BEIJING BAIAO PHARMA
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