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Preparation method of chitosan tubular bracket

A technology of chitosan and chitosan solution, which is applied in the fields of biomedical materials and tissue engineering, can solve problems such as lack of research reports, unevenness, and difficulty in preparing chitosan tubes, and achieve firm suture connections, good elasticity and The effect of strength and simple process

Inactive Publication Date: 2007-02-07
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, chitosan has no melting point, and it can only be carbonized when heated, but cannot be softened or melted, so it cannot be processed into tubes by extrusion or other methods.
The Chinese patent (ZL99123745.5) "Chitosan catheter for nerve repair" discloses the method of preparing chitosan tubes by rotary evaporation, but this method is time-consuming and laborious, and the prepared tubes are non-porous and uneven, and Difficult to prepare chitosan tubes longer than 20mm
However, there is no research report on the direct application of industrial textile technology to process chitosan yarns to prepare tubular scaffolds for tissue engineering

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Two 21-count chitosan yarns are plyed together, and the warp direction braiding method is used to adjust the appropriate tension and walking speed, and weave into a hollow network tube with an inner diameter of 3mm and a wall thickness of about 1.0mm;

[0025] 2) Take the hollow network pipe 300mm, and insert a stainless steel mandrel with a diameter of 3mm and a circular cross section into the hollow network pipe;

[0026] 3) Take 1 gram of chitosan with a deacetylation degree of 83% and dissolve it in 100 ml of 1% acetic acid solution, stir and dissolve, and prepare a 1% (w / v) chitosan solution. After the chitosan is completely dissolved, filter with 200-mesh nylon cloth, depressurize and pump air at room temperature to eliminate the bubbles in the solution;

[0027] 4) Immerse the chitosan tube supported by the mandrel into the chitosan solution from which air bubbles have been removed, rotate slowly, and take it out vertically.

[0028] 5) Place the chitosan tub...

Embodiment 2

[0033] 1) Two chitosan yarns of 21 counts are plyed, and the appropriate tension and walking speed are adjusted by the method of warp direction weaving, and a hollow network tube with an inner diameter of 6 mm and a wall thickness of about 1.0 mm is woven;

[0034] 2) Take 100 mm of the hollow network pipe, and insert a polytetrafluoroethylene mandrel with a diameter of 6 mm and a circular section into the hollow network pipe.

[0035] 3) Take 3 grams of chitosan with a deacetylation degree of 90% and dissolve it in 100 ml of 5% acetic acid solution, stir and dissolve, and prepare a 3% (w / v) chitosan solution. After the chitosan is completely dissolved, it is filtered with 200 mesh nylon cloth. Then prepare 100 ml of 20% (w / v) gelatin aqueous solution, mix the two, heat and stir at 50° C. until uniform, and pump air under reduced pressure at room temperature to eliminate air bubbles in the solution.

[0036] 4) Immerse the chitosan tube supported by the mandrel into the chito...

Embodiment 3

[0042] 1) With 21 pieces of chitosan yarn, 4 strands are plyed, and the appropriate tension and walking speed are adjusted by the method of warp direction weaving, and the hollow network tube with an inner diameter of 1 mm and a wall thickness of about 0.5 mm is woven;

[0043] 2) Take the hollow network pipe 200 mm, insert a rod-shaped mandrel with a diameter of 1 mm, a circular cross section, a stainless steel rod as the inner core, and a polytetrafluoroethylene tube as the outer layer, into the hollow network pipe;

[0044] 3) Take 2 grams of chitosan with a deacetylation degree of 93% and dissolve it in 100 ml of 1% acetic acid solution, stir and dissolve, and prepare a 2% (w / v) chitosan solution. After the chitosan is completely dissolved, filter with 200-mesh nylon cloth, and depressurize at room temperature to remove air bubbles in the solution.

[0045] 4) Immerse the chitosan tube supported by the mandrel into the chitosan solution from which air bubbles have been rem...

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PUM

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Abstract

A preparation method of chitosan tubular stent, which uses chitosan yarn as raw material, preparing hollow net tube structures of different specifications by means of spinning, and then inserting corresponding core model, coating chitosan, collagen, or gelatin biomacromolecule liquor, withdrawing the core model after drying, and obtaining a chitosan tube with wall thickness of approximately 0.5~2.5mm and inside diameter of 1~20mm. The method is simple and easy, and is suitable for mass production. The chitosan tube prepared by the invention has the characteristics of thick wall, controllable inside diameter, optional length, modeling in one go, good elasticity and intensity, and firm saturation in operation. All the raw materials in the preparation process adopts natural biodegradable materials, with their metabolic products having no toxic and side effects on human body, and are suitable for being used as tissue repair materials or tissue engineering stent materials.

Description

technical field [0001] The invention relates to a preparation method of a chitosan tubular support, which belongs to the technical field of biomedical materials and tissue engineering. Background technique [0002] In the field of regenerative medicine and tissue engineering, tubular objects can be used as scaffolding materials for repair of injuries to various organs and tissues, such as artificial nerve tubes (including repair catheters for peripheral nerve injuries and repair catheters for spinal cord injuries), artificial trachea, artificial blood vessels etc. The use of biodegradable materials to prepare stents can avoid secondary operations and reduce the pain of patients. Synthetic biodegradable materials include polylactic acid (PLA), polyglycolic acid (PGA), copolymer of glycolic acid and lactic acid (PLGA), polycaprolactone (PCL), and the like. Synthetic materials have the advantage of being easy to process and shape. However, the synthesis process is complicated...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/04A61L27/20A61L29/04
Inventor 张秀芳王爱军公衍道敖强曹文灵奚静赵畅
Owner TSINGHUA UNIV