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Compound lysoamidase spray agent and preparing method

A technology of spray and lysozyme, applied in the field of compound lysozyme Lysoamidase spray and preparation, can solve the problems of instability of Lysoamidase, application impact, development limitations, etc. Effect

Inactive Publication Date: 2007-05-16
SHANGHAI CENT OF RES & DEV OF NEW DRUGS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, because Lysoamidase is unstable in aqueous solution, its formulation development is limited, and its application is also affected.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Lysoamidase 50,000IU (about 300mg)

[0025] Vitamin C Palmitate 1.5g

[0026] Tartaric acid 0.28g

[0027] Menthol 0.1g

[0028] Aspartame 0.15g

[0029] Sodium malate 0.5g

[0030] Prepare 0.01M, PH8.0 phosphate buffer (100ml) (sodium dihydrogen phosphate + disodium hydrogen phosphate); add compound lysozyme Lysoamidase (50,000IU), stir to dissolve and disperse evenly; add vitamin C palmitate , sodium malate, tartaric acid, menthol and aspartame, stirred to speed up the dissolution to make a stable system, filtered and put into a fully sterilized spray container, and stored at a low temperature of 4-6°C.

Embodiment 2

[0032] Lysoamidase 50,000IU(300mg)

[0033] Thiourea 3.8g

[0034] Diethylamine tetraacetic acid sodium salt 0.5g

[0035] Lemon Oil 0.18g

[0036] Stevia 0.15g

[0037] Prepare 100ml of phosphate buffer solution of pH6.8 (disodium hydrogen phosphate+potassium dihydrogen phosphate); add compound lysozyme Lysoamidase, stir to make it dissolve, and disperse evenly; add thiourea, diethylamine tetraacetic acid sodium salt, lemon oil and Stevioside is prepared into a solution, stirred to accelerate dissolution, and made into a stable system, filtered, divided into sterilized spray containers, and stored at a low temperature of 4-6°C.

Embodiment 3

[0039] Lysoamidase 50,000IU

[0040] L-Glutathione 2.5g

[0041] Citric acid 1.2g

[0042] Rose Oil 0.28g

[0043] Stevia 0.3g

[0044] Bovine Albumin 0.5g

[0045] Prepare 100ml of Tris buffer (Tris+hydrochloric acid buffer) at pH 5.0; add compound lysozyme Lysoamidase, stir to dissolve and disperse evenly; add L-glutathione, citric acid, rose oil, stevia and bovine white The protein is made into a solution, stirred to accelerate the dissolution, and made into a stable system, filtered, divided into sterilized spray containers, and stored at a low temperature of 4-6°C.

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Abstract

The compound lysoamidase spray agent of the present invention contains lysoamidase as the active component in 0.1-1.0 %, antioxidant in 0.1-10.0 % and antioxidant synergist 0.1-10.0 %, and has pH 3.0-9.0 buffering liquid as solvent. The recipe of the present invention solves the problem of stability of compound lysoamidase in water solution, and the provided spray agent has convenient use, obvious antiseptic effect and stable quality.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations. It specifically relates to a compound lysozyme Lysoamidase spray and a preparation method. Background technique [0002] Lysoamidase is a new type of medical or veterinary antibacterial enzyme external drug obtained from the culture solution of a single bacillus, which has a strong ability to dissolve bacteria and proteins. Lysoamidase powder is hygroscopic, odorless and tasteless. Soluble in water, insoluble in ethanol and chloroform. Lysoamidase is composed of 90% polysaccharide and 10% protein (polysaccharide is negatively charged, and has many unit structures composed of N-acetylglucosamine, N-acetylmannuronic acid and N-acetylgalacturonic acid. Protein Partly consists of nearly 20 different proteins, including lysoproteins such as phosphatases and proteases. The original main dosage form of Lysoamidase is freeze-dried powder, which can be used as a topical drug for su...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61K9/12A61P31/04
Inventor 韩庆惠李连会
Owner SHANGHAI CENT OF RES & DEV OF NEW DRUGS
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