Nerve growth factor cDNA, clone and expresion of Chinese cobra

Abstract

The invention is cDNA, cloning and expression of Chinese cobra neural growth factor (NGF), relating to cDNA and cloning of recombinant Chinese cobra neural growth factor, expression of neural growth factor, a method of using its expression carrier to prepare it and pharmacological use of the mentioned protein, separate-extracting total RNA from the poison gland of Chinese cobra, using RT-PCR method to make reverse transcription to get total cDNA, using the primer designed by us to provide a specific Cdna sequence at a length of 348 bp polynucleotide sequence and also providing a small-molecular weight protein, the molecular weight about 13000, successfully expressed in pichia pastoris. The recombinant neutral growth factor has pharmaceutical use in the aspects of reducing blood sugar and improving pancreas islet as well as curing peripheral polyneuritis caused by diabetes.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to the cDNA and clone of recombinant Chinese cobra (Chinesecobra [Naja naja atra]) nerve growth factor (nerve growth factor, NGF), the expression of the nerve growth factor and the method for preparing the nerve growth factor by using its expression vector and pharmaceutical uses of said protein. technical background

[0002] Nerve growth factor (NGF) is one of the most important biologically active proteins in the nervous system. It mainly acts on the nervous system, such as sympathetic neurons, sensory neurons and forebrain cholinergic neurons. NGF participates in regulating their development and differentiation, maintaining their normal functions, and promoting their repair after injury. At present, NGF has shown important clinical application value in promoting the repair of nerve injury and treating some neuron degenerative diseases such as Alzheimer's disease. At present, NGF has ...

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