Production process of natural marine organism health food with deep sea clam worm as material
A technology for marine organisms and health food, applied in food preparation, food science, capsule delivery, etc., to prevent thrombosis, scientific extraction methods, and good effects of regulating blood pressure and blood lipids
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Embodiment 1
[0046] 1. Take the fresh clamworm Perinereris aibuhitensis of the family Nereididae obtained from the deep sea, quickly bring it back to the room, and keep it in salt water for 3-4 days to make it spit out the contents;
[0047] 2. Add phosphate buffer and homogenize in a homogenizer;
[0048] 3. Put the homogenate into a centrifuge for centrifugation at 3000rpm, centrifuge at 5°C for 20min, and collect the supernatant;
[0049] 4. Weigh a certain amount of crushed ammonium sulfate, add it to the above supernatant to make 30% saturation, and after standing at 4°C for 10 hours, centrifuge to collect the supernatant; add ammonium sulfate to 70% saturation, Let stand overnight, and centrifuge to collect the precipitate;
[0050] 5. Dissolve the above precipitate with phosphate buffer solution, and desalt through dialysis or chromatography column to obtain the desalted solution;
[0051]6. The above-mentioned desalted solution is subjected to ultrafiltration treatment through an...
Embodiment 2
[0055] 1. Take fresh clam worms of Marphysa sanguinea, a rock worm of the family Eunicidae, from the deep sea, and quickly bring them back indoors, and maintain them in salt water for 3-4 days to make them spit out the contents;
[0056] 2. Add phosphate buffer and homogenize in a homogenizer;
[0057] 3. Put the homogenate into a centrifuge for centrifugation at 14000rpm, centrifuge at 10°C for 5min, and collect the supernatant;
[0058] 4. Weigh a certain amount of crushed ammonium sulfate, add it to the above supernatant to make 30% saturation; after standing for 5 hours, centrifuge to collect the supernatant; add ammonium sulfate to 70% saturation, and let stand overnight , to collect the precipitate by centrifugation;
[0059] 5. Dissolve the above precipitate with phosphate buffer solution, and desalt through dialysis or chromatography column to obtain the desalted solution;
[0060] 6. The above-mentioned desalted solution is subjected to ultrafiltration treatment thr...
Embodiment 3
[0064] 1. Take the fresh clamworm Arenicola brasiliensis of the family Arenicolidae obtained from the deep sea, quickly bring it back indoors, and keep it in salt water for 3-4 days to make it spit out the contents;
[0065] 2. Add phosphate buffer and homogenize in a homogenizer;
[0066] 3. Put the homogenate into a centrifuge and centrifuge at 10000rpm, centrifuge at 10°C for 10min, and collect the supernatant;
[0067] 4. Weigh a certain amount of crushed ammonium sulfate, add it to the above supernatant to make 30% saturation; after standing for 2 hours, centrifuge to collect the supernatant; add ammonium sulfate to 70% saturation, and let stand overnight , to collect the precipitate by centrifugation;
[0068] 5. Dissolve the above precipitate with phosphate buffer solution, and desalt through dialysis or chromatography column to obtain the desalted solution;
[0069] 6. The above-mentioned desalted solution is subjected to ultrafiltration treatment through an ultrafil...
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