Method of regulating degradation rate of porous collagen-based cradle with amino acid

A degradation rate, porous scaffold technology, used in medical science, prosthesis, surgery, etc., can solve problems such as difficulty in regulating cross-linking degree, cytotoxicity, and inability to meet the requirements of various tissues and organs with different regeneration rates. To achieve the effect of simple and easy preparation process, low cost, good biocompatibility and degradation performance

Inactive Publication Date: 2003-11-05
ZHEJIANG UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the traditional pure chemical cross-linking method is that it is usually difficult to control the degree of cross-linking and cannot meet the requirements of various t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of regulating degradation rate of porous collagen-based cradle with amino acid
  • Method of regulating degradation rate of porous collagen-based cradle with amino acid
  • Method of regulating degradation rate of porous collagen-based cradle with amino acid

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1

[0019] The effect of different kinds of amino acids on the degradation of collagen-based porous scaffolds.

[0020] Place the type I collagen scaffold (2.5mg / piece) derived from beef tendon in a 24-well culture plate, and add 1ml of 2.5μM glycine (Gly), glutamic acid (Glu) or lysine (Lys) respectively. 2-N-morpholine ethane sulfonic acid (MES) solution (50 mM, pH 5.5), soak for 1 hour; at the same time, add three-distilled water as a control. Then add 1ml of MES solution containing 40mM 1-ethyl-3-(dimethylaminopropyl)-carbodiimide (EDAC) and 40mM N-hydroxysuccinimide (NHS) to make the final The concentrations of EDAC and NHS are 20mM and 10mM, respectively. Cross-link at room temperature for 24 hours, after cross-linking, rinse with three-distilled water 6 times for 10 minutes each time. After freeze-drying, the cross-linked collagen-based porous scaffold is obtained.

[0021] Take a collagen-based porous scaffold without cross-linking, no amino acid cross-linki...

Example Embodiment

[0022] Example 2

[0023] The effect of the concentration of glutamic acid on the degradation rate of the collagen-based porous scaffold.

[0024] Place the type I collagen scaffold (2.5mg / piece) derived from bovine tendon in a 24-well culture plate, add 1ml of 1-10μM glutamic acid MES solution (50mM, pH 5.5), soak for 1 hour; then 1 ml of MES solution containing 40 mM EDAC and 40 mM NHS was added to make the final EDAC and NHS concentrations respectively 20 mM and 10 mM. Cross-link at room temperature for 24 hours, after cross-linking, rinse with three-distilled water 6 times for 10 minutes each time. After freeze-drying, the cross-linked collagen-based porous scaffold is obtained.

[0025] Take the porous scaffolds cross-linked with different concentrations of glutamic acid, add 3 ml of 0.5 mg / ml type I collagenase solution (PBS, pH 7.4), and digest in a constant temperature water tank at 37° C. for 12 hours. 1ml of the supernatant was removed by aspiration, placed in a polymeri...

Example Embodiment

[0026] Example 3

[0027] The effect of lysine concentration on the degradation rate of collagen-based porous scaffolds.

[0028] Place the type I collagen scaffold (2.5 mg / piece) derived from bovine tendon in a 24-well culture plate, add 1 ml of 0.67-100 μM lysine MES solution (50 mM, pH 5.5), and soak for 1 hour; then 1 ml of MES solution containing 40 mM EDAC and 40 mM NHS was added to make the final EDAC and NHS concentrations respectively 20 mM and 10 mM. Cross-link at room temperature for 24 hours, after cross-linking, rinse with three-distilled water 6 times for 10 minutes each time. After freeze-drying, a cross-linked collagen-based porous scaffold is obtained.

[0029] Take the porous scaffold cross-linked with different concentrations of lysine, add 3ml of 0.5mg / ml type I collagenase solution (PBS, pH7.4), and digest it in a constant temperature water tank at 37°C for 12h. 1ml of the supernatant was removed by aspiration, placed in a polymerization tube, 2ml of 6M hydroc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Amino acid with high biocompatibility is used as cross-linking and bridging agent, amino acid in 0.1-100 microns is made to compound with porous collagen-based cradle, and the cradle is further cross-linking treated with carbonated diimine compound, so as to prepare porous collagen-based cradle with controllable degradation rate and good biocompatibility. The present invention uses material withwide source, low cost and no toxic side effect; and the preparation process is simple, feasible, mild in condition and good in repeatability. The prepared porous collagen-based cradle can meet the requirement for different tissue and organ to regenerate and may be used widely in regeneration and reconstruction of skin, cartilage, bone, blood vessel, nerve, tendon, heart valve and other organs inbiomedicine, tissue engineering and other fields.

Description

technical field [0001] The invention relates to a method for using amino acid as a cross-linking bridging agent to regulate the degradation rate of a collagen-based porous scaffold under the action of the cross-linking agent. Background technique [0002] Tissue engineering involves many disciplines and fields such as medicine, chemistry, biology, and materials science. In the study of tissue engineering, the selection of scaffold materials and the construction of scaffolds are the key links. Collagen is the main component of connective tissue in mammals, constituting about 30% of the protein in the human body and 72% of the dry weight in the skin. There are 19 types of collagen, the most common being types I, II, and III. Among them, type I is the most abundant and has excellent performance, and is widely used in biomaterials. Although collagen materials have unparalleled biocompatibility, the mechanical strength of scaffolds constructed of pure...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/00A61L27/24A61L27/50A61L31/00
Inventor 高长有马列毛峥伟沈家骢
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap