Preparation method of CdSe nano-crystalline composite liposome microcapsule bubble used for fluorescence immunity detection

Abstract

The present invention belongs to the field of nano material and biological technology. The composite nano CdSe liposome microvesicle for fluorescent immunoassay is prepared through three steps of: preparing nano oily CdSe semiconductor crystal, synthesizing composite nano oily crystal microvesicle, and electrostatically connecting microvesicle with biological protein via altering pH value to regulate surface potential. The present invention is mainly used in test paper strip for immunochromatographic detection of hepatitis B virus, pesticide residue in vegetable and drug detection. The lipophilic surface nano CdSe crystal is phase transferred to constitute biocompatibility.

Description

technical field [0001] The invention belongs to the field of nanomaterials and biotechnology, and relates to nanobiotechnology, in particular to a method for preparing CdSe nanocrystal composite liposome microvesicles used for fluorescence immunity detection. Background technique [0002] For biological and biomedical detection, fluorescent nanocrystals are a kind of fluorescent probe with potential application value. Traditional fluorescent probes generally use organic dyes. As a biological detection probe, organic dyes have many disadvantages, mainly in that the half-peak width of the fluorescence spectrum emitted by organic dyes is about 3-5 times the half-peak width of the fluorescence spectrum emitted by fluorescent nanocrystals. overlap, which affects the sensitivity of detection, and the excitation wavelength of organic dyes is narrower than that of fluorescent nanocrystals, which limits the range of excitation light sources, that is, a dye must be excited by a specif...

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Problems solved by technology

However, the disadvantage of these two methods is that the fluorescent nanocrystals and protein will aggregate and settle during the coupling process of fluorescent nanocrystals and proteins.

In addition, for thiol-modified quantum dots, the thiol group is excessive, so that the protein will bind to the free thioglycolic acid in the solution during the interaction of the protein with thioglycolic acid, which will affect the detection sensitivity.

Then in 2000, Nie used polystyrene beads to coat the fluorescent nanocrystals. However, due to the swelling problem of the polystyrene beads in the solution, the fluorescent nanocrystals leaked, which also limited the ability of the nanocrystals. Applications

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