Production process of microbial organic composite fertilizer

A technology of microorganisms and crops, applied in the field of manufacturing technology of microbial organic compound fertilizers, can solve problems such as air pollution, waste of resources, fire hazards, etc., and achieve the effect of improving soil structure and reducing pollution

Inactive Publication Date: 2004-11-24
上海汇仁复合有机肥料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] China's organic fertilizer resources are very rich: first, more than 570 million tons of crop straw are produced every year, and more than a quarter of the straw cannot be processed. It is piled up or burned in the field, which not only wastes resources,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Cultivation of Azotobacter ohroococcum

[0011] a. Primary slant cultivation

[0012] The main ingredients of the culture medium are as follows: 1% sucrose, 0.05% dipotassium hydrogen phosphate, 0.1% calcium carbonate, 0.02% magnesium sulfate, 0.02% sodium chloride and 2% agar. PH7-7.2.

[0013] Put the culture medium into a test tube according to the conventional method, sterilize, inoculate, and incubate at 28±1°C for 24 hours and save it for later use.

[0014] b. Secondary liquid culture

[0015] Remove the agar in the main ingredient formula of the primary medium, and use it as the main ingredient of the secondary medium, pH7-7.2.

[0016] Prepare the culture medium and put it into the Erlenmeyer flask. After sterilizing and cooling, inoculate the slant strains in the liquid culture medium; shake and culture at 28±1°C for 24 hours, when the number of cells reaches 500 million / g, Terminate the culture and reserve.

[0017] c. Tertiary solid culture

[0018] Th...

Embodiment 2

[0021] Cultivation of nitrogen-fixing bacterium Trichoderma koningii

[0022] a. Primary training

[0023] The formula is conventional potato plus agar medium.

[0024] Put the prepared culture medium in a test tube, inoculate it after routine sterilization, and cultivate it at 26±1°C for 3-4 days; after green spores grow, stop the cultivation and save it for later use.

[0025] b. Secondary training

[0026] The medium formula is: bran 50%, cornmeal 40%, straw powder 10%.

[0027] After moistening, sterilizing and cooling the dry material, inoculate the first-grade bacteria and culture it in a glass petri dish. Cultivate at 26±1°C for about 5 days; stop the cultivation after green spores grow. Dry at low temperature (below 60°C) and save for future use.

[0028] c. Tertiary training

[0029] The medium formula is the same as the secondary medium formula.

[0030] After moistening, sterilizing, and cooling the dry material, inoculate the secondary strain, and use the th...

Embodiment 3

[0032] Cultivation of Bacillus megatherium phosphaticum

[0033] a. Primary slant cultivation:

[0034] The medium formula is conventional potato plus agar medium. Sterilize according to conventional methods, inoculate, incubate at 28±1°C for 48 hours, and store for future use.

[0035] b. Secondary liquid culture:

[0036] Remove the agar from the primary medium formulation and use it as a secondary medium.

[0037] Put the culture medium into the Erlenmeyer flask, inoculate the slant strain after autoclaving and cooling, and culture on a shaking table at 28±1°C for 36 hours. When the number of cells reaches 500 million / g, stop the culture and set aside.

[0038] c. Tertiary training

[0039] The medium formula is: bran 30%, sorghum 20%, cornmeal 40%, fly ash 10%.

[0040] Weigh the dry material, moisten it, sterilize it, cool it down, connect it with secondary strains, and culture it on a shallow plate for 48 hours at 28±1°C, and stop the culture when the number of cell...

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Abstract

The production process o microbial organic composite fertilizer includes the following steps: collecting and crushing animal excrement, hyacinth, kitchen refuse, human excrement, crop stalk and wood dust; inoculating beneficial microbe actinomycetes, bacteria, fungi, etc. and fermentation to raise the temperature to 65 deg.c to kill germs and parasite egg; deodorizing and dewatering; adding azotobacteria, phosphate decomposing bacteria, potash decomposing bacteria, etc and fermentation in green house; drying, sieving and others. The present invention has the advantages of reducing pollution and improving soil structure.

Description

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Claims

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Application Information

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Owner 上海汇仁复合有机肥料有限公司
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