Flavour development capacity improved glucosamine hydrochloride solution, preparation method thereof, combination and food of glucosamine hydrochloride
A technology of glucosamine salt and glucosamine, which is applied in the field of glucosamine saline solution and can solve problems such as use restrictions
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preparation example Construction
[0052] In the preparation method of glucosamine saline solution of the present invention, because use the glucosamine strong salt of glucosamine hydrochloride or sulfate etc. with aqueous solution, therefore in the technological process of preparing glucosamine, hydrolyze chitin with concentrated hydrochloric acid The obtained aqueous solution of glucosamine hydrochloride before recrystallization can also be used as a raw material as it is. In this case, the recrystallization process and drying process of glucosamine hydrochloride can be omitted, which can improve work efficiency and reduce cost.
[0053] In the following examples, the amount of glucosamine is measured using the Rondle-Morgan quantitative method, the amount of organic acids is measured using high performance liquid chromatography (column: Shimadzu Shim-PakSCR-102H), and the amount of chloride ion The content is measured by molar titration.
Embodiment 1
[0057] The glass column (Ishii Rikaki Mfg. Co., Ltd.) with a length of 30 cm, a diameter of 3 cm, and a volume of 210 ml in the resin filling part was plugged with gauze, and about 2 cm of sea sand was added from below. There is a silicon tube at the outlet of the column, a three-way cock is set at the head of the silicon tube, and one end is connected to a separatory funnel set at a position higher than the column, which can be used to backwash the inside of the column. The other end is connected to a Pasteur pipette as an outlet. The tubing connecting each container is fitted with spring clips to allow flow adjustment. The strongly acidic cation exchange resin (Muromac XSC-1123, Muromachi Technos Co., Ltd.) of 100ml is packed in the column, and the 2N hydrochloric acid (its flow velocity is SV=2.1) that flows into 150ml, then flows into 500ml ultrapure water (its flow velocity is SV = 6.0). After backwashing with 150 ml of ultrapure water, 400 ml of 0.5 mol / L glucosamine h...
Embodiment 2
[0061] The length of the resin filling part is 40cm, the diameter is 6cm, and the glass column (VIDREX Co., Ltd.) with a capacity of 1130ml is plugged with gauze, and 1000ml type I strong basic anion exchange resin (MuromacXSA-2123, Muromachi Technos Co., Ltd.) is filled in the column. Company), flow into 5L of 0.5 mol / L sulfuric acid (the flow rate is SV=2.9), then flow into 10 L of ultrapure water (the flow rate is SV=6.0), and then inject 10L of 1 mol / L sodium hydroxide solution (the flow velocity thereof is SV=2.9), and then flow into 10 L of ultrapure water (the flow velocity thereof is SV=6.0) to regenerate the resin. Subsequently, 10 L of 1 mol / L citric acid was flowed in (at a flow rate of SV=3.3) to immobilize it on the resin. To remove excess citric acid, flow 11L of ultrapure water. Then flow into 5L of 10% (g / ml) glucosamine hydrochloride (flow velocity is SV=1.2), collect the effluent samples at this time by every 250ml, and it is numbered sequentially from No.1,...
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