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Chinese forest frog bactericidal peptide spray agent and its preparation

An antibacterial peptide and spray technology are applied in the field of antibacterial and analgesic drugs and their preparation to achieve the effects of good therapeutic effect, easy popularization and use, and avoidance of first-pass effect.

Inactive Publication Date: 2005-02-23
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent has not made Rana antimicrobial peptides into drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 The impact of the dosage of Rana antimicrobial peptide on antibacterial activity

[0025] Select 2.0 grams of ethylparaben, polyethylene glycol 400 (PEG 400 ) 10 grams, sodium chloride 2.0 grams, first dissolve ethylparaben in water for injection, heat to about 45 ° C, add PEG 400 , sodium chloride, stirred to make it completely dissolved, and made into four parts of auxiliary material solution after high temperature sterilization; The water for injection is prepared into Rana antimicrobial peptide sprays which account for about 5%, 15%, 30% and 40% of the Rana antimicrobial peptide by mass.

[0026] The antibacterial activity of Pseudomonas aeruginosa was determined by the tube-and-disc method, and the results showed that: with the increase of the dosage of Rana antibacterial peptide, the antibacterial activity was also enhanced; however, the enhancement of antibacterial activity was slow when the dosage exceeded 35%.

[0027] The best dosage of raw mate...

Embodiment 2

[0033] Embodiment 2 optimal proportioning, zone of inhibition test, stability test

[0034] 1. The optimal dosage of raw materials and auxiliary materials for Rana antimicrobial peptide spray is as follows:

[0035] (1) Rana antimicrobial peptide is 18%;

[0036] (2) Ethylparaben is 1%;

[0037] (3) polyethylene glycol 400 is 2.5%;

[0038] (4) Sodium chloride is 0.6%.

[0039] (5) The balance is water for injection.

[0040] 2. Zone of inhibition test

[0041] The following experiment is to use the Rana antimicrobial peptide spray prepared in Example 2

[0042] (1) Strains

[0043] Pseudomonas aeruginosa ATCC27853

[0044] Escherichia coli Escherichia coli ATCC25922

[0045] Staphylococcus aureus Staphylocouus aureus ATCC25923

[0046] Pseudomonas aeruginosa with clinical multi-drug resistance (the main drug-resistant names are piperacillin, ofloxacin, cefotaxime, sulfamethoxazole, third-generation cephalosporin, and peridone)

[0047] Clinical multidrug-resistant S...

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PUM

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Abstract

A spray of tree frog's antibacterial peptide for treating burn, scald, and skin infection is prepared from antibacterial peptide of tree frog, nipagin ethylester, PEG 400, sodium chloride and water through preparing auxiliary solution, adding the antibacterial peptide of tree frog and water, stirring and loading in sprayer.

Description

technical field [0001] The invention belongs to the field of an antibacterial and analgesic drug and its preparation, and in particular relates to a Rana antibacterial peptide drug and a method for preparing a Rana antimicrobial peptide spray. Background technique [0002] Antimicrobial peptides (AMPs) are an important part of the internal defense system of organisms. Antimicrobial peptides have the ability to resist Gram-positive bacteria, Gram-negative bacteria, fungi, coated viruses, protists and drug-resistant bacteria, and have been widely used in food preservation and medicine fields. [0003] Amphibians live in complex and diverse environments, and their skin plays an important role in maintaining their survival, colonizing and adapting to their vast habitats. In order to adapt to the living environment, a wide variety of active substances with complex functions are distributed in the skin of amphibians, which is a huge resource bank of active biomolecules and drug r...

Claims

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Application Information

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IPC IPC(8): A61K9/12A61K38/17A61P17/02A61P31/02
Inventor 李青山滕利荣孟庆繁田晓乐周杰
Owner JILIN UNIV
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