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Polypeptide vaccine capable of effectively preventing pointed condyloma

A technology of condyloma acuminatum and polypeptide vaccine, applied in the field of biomedicine, can solve the problems of large tissue damage, inability to completely remove viruses, local ulcers, etc., achieve significant immunogenicity and antigenicity, facilitate automated mass production, and have obvious immune effects. Effect

Inactive Publication Date: 2005-03-23
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] ①Surgical therapy: Condyloma acuminatum is generally not advocated for surgical excision, because after surgical treatment, condyloma acuminatum is easy to recur, making the treatment fail
[0009] ① Podophyllum lipid: It is suitable for genital warts in wet areas, but it has many disadvantages, such as great damage to tissues, and improper use can cause local ulcers
[0017] None of the above treatments and / or drugs can completely eliminate the replication of the virus in the body, so the recurrence rate is high

Method used

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  • Polypeptide vaccine capable of effectively preventing pointed condyloma
  • Polypeptide vaccine capable of effectively preventing pointed condyloma
  • Polypeptide vaccine capable of effectively preventing pointed condyloma

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0073] Experimental example 1, the experimental results of the basic amino acid sequence of the present invention immunized mice

[0074] Main reagents and their preparation

[0075] Coating diluent (0.05mol / L sodium carbonate-sodium bicarbonate buffer, pH9.6) composition:

[0076] Na2CO3 1.5g, NaHCO3 2.9g, Na2N3 0.2g, add double distilled water to 1000ml, adjust to pH9.6

[0077] Blocking solution (5% calf serum / PBS solution) composition:

[0078] Calf serum 50ml, add PBS (pH7.4) 950ml.

[0079] Phosphate buffered saline (PB):

[0080] A liquid (0.2mol / L sodium dihydrogen phosphate aqueous solution) composition: NaH2PO4·H2O 27.6g dissolved in ultrapure water 1000ml.

[0081] B solution (0.2mol / L disodium hydrogen phosphate aqueous solution) composition: Na2HPO4·7H2O 53.6g (or Na2HPO4·12H20 71.6g or Na2HPO4·2H2O 35.6g) dissolved in ultrapure water 1000ml. Sample diluent (PBS, 0.01mol / L phosphate-buffered saline) components: PB A solution 19ml; PB B solution 81ml; NaCl 18....

experiment example 2

[0101] Experimental example 2, the antibody of immunized mice reacted with the supernatant of condyloma acuminatum

[0102] The method of indirect enzyme-linked immunosorbent immunoassay (ELISA) is the same as in Experimental Example 1. Take the wart tissue of condyloma acuminatum, repeatedly freeze, homogenize, ultrasonically crush, dissolve in PBS liquid, take the supernatant liquid after centrifugation, react with mouse serum, and use the method of ELISA The detection and identification methods are the same as in Experimental Example 1, and the results are represented by the average OD value of double wells. If the OD value of the specimen to be tested / the OD value of the negative control > 2.1, it is determined as positive.

experiment example 3

[0103] Experimental example 3, the synthesis of polypeptide vaccine

[0104] Adopt the standard Fmoc scheme, initially select 0.0125mmol, PSC resin (produced by ABI company, batch number A5F013), respectively according to the sequence characteristics described in claim 1, the sequence characteristics described in claim 2, and the sequence characteristics described in claim 3 , the sequence feature described in claim 4, the sequence feature described in claim 5, the peptide chain is extended from the C terminal to the N terminal one by one, and the consumption of each amino acid raw material (produced by U.S. ABI company) is 0.1mmol. Various amino acid protection groups are: the α amino group of each amino acid is protected by Pmoc, and the other side chain protection groups are Arg (Mtr), tyr (tBu), Thr (tBu), Tyr (tBu), Asp (OtBu). Each step of condensation adds HoBt / Dcc activation to protect the carboxyl group of the amino acid. Use NMP solution containing 20% ​​hexahydropy...

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Abstract

A polypeptide vaccine for preventing pointed condyloma features that its basic amino acid sequence is amino-KPTPEKEKPDPYKNL-carboxy, and the Lys is used to serially linking multiple said basic amino acid sequences.

Description

(1) Technical field [0001] The invention relates to the field of biomedicine, in particular to a polypeptide vaccine for preventing condyloma acuminatum. (2) Technical background [0002] At present, the treatment of condyloma acuminatum can only adopt comprehensive treatment methods, which can be summarized as follows: [0003] ①Surgical therapy: Condyloma acuminatum generally does not advocate surgical excision, because after surgical treatment, genital warts are easy to recur, which makes the treatment fail. [0004] ②Cryotherapy: Use liquid nitrogen at a low temperature of -196°C to treat condyloma acuminata by pressing freezing to promote the necrosis and shedding of wart tissue. This method is suitable for condyloma with a small number and small area. It is feasible to treat 1 or 2 times with an interval of one week . [0005] ③Laser treatment: CO2 laser is usually used to treat genital warts by cauterization. [0006] ④ Electrocautery: Use high-frequency electroacu...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K39/00A61P17/12
Inventor 吕凤林
Owner INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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