Enzymolysis method for preparing protein oligopeptide powder using Pacific Ocean alaska pollack

Abstract

One kind uses the Pacific Ocean Alaska pollack fish flesh preparation protein widowed peptide powder the medium technique, its operation technology method is: After the Pacific Ocean Alaska pollack fish flesh will twist garrulously, will increase the 20AU/kg~~80AU/kg bacillus subtilus inscribe proteinase and the 8000LAPU/kg~~40000LAPU/kg tune mold circumscribe proteinase; Or increases the 20AU/kg~80AU/kg bud spore bacillus to conform to the proteinase, with 1.2: 1~1: 1.5 water even mixes, in the enzyme reactor enzymolysis 2hr~12hr, enzymolysis temperature 50deg.C~60deg.C, outset PH is equal to 6.6, the enzymolysis fluid passes through deactivation, the centrifugal separation, the membrane separation, again spray drying or drying. Then obtains the water-soluble protein widowed peptide powder and the wastage. The invention preparation protein widowed peptide powder is the pasteurized milk white, does not have the bitter taste, the micro fish fishy smell, the molecular weight (is bigger than 85%) to be possible separately to control for is smaller than was equal to 1000Da and 1000~5000Da, the protein widowed peptide powder returns-ratio (relative raw material total protein) achieves 65%~79%.

Description

Technical field: [0001] The invention belongs to the enzymatic production process technology of marine bioengineering, and relates to a process method for preparing protein oligopeptide powder by enzymatically hydrolyzing Pacific pollock flesh. Background technique: [0002] Protease enzymatic hydrolysis is the most promising process for preparing protein oligopeptide products. The economic quality indicators such as recovery rate, molecular weight (closely related to biological activity), taste and flavor of protein oligopeptide products mainly depend on the type of protease preparation selected, Raw material species, enzymatic hydrolysis parameters (E / S, enzyme weight or enzyme activity unit / kg protein, material-water ratio, time, temperature) and other conditions, the results can show great differences. Before the present invention was made, French F.Guerard et al. (2001, 2002) used Alcalase protease and "Umamizyme" neutral protease (E / S=1g / kg~15g / kg, material-water ratio...

AI Technical Summary

Problems solved by technology

At present, there is no report on the enzymatic hydrolysis process method for preparing protein oligopeptide powder using Pacific pollock as a specific raw material. Optimal screening of enzymatic hydrol...