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Oral freeze dried formulation of specific yelk immunoglobulin and its preparation method and usage

A technology of egg yolk immunoglobulin and immunoglobulin, which is applied in freeze-dried transportation, antiviral agents, digestive system, etc., can solve the problems of easy degradation of egg yolk immunoglobulin antibody oral liquid and destruction of antibody activity, and achieve cost reduction, Loss reduction and low cost effects

Inactive Publication Date: 2005-06-01
SHENZHEN ANTUOXING BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention solves the technical problems that the spray drying production method in the background technology destroys the activity of yolk immunoglobulin antibody and the oral liquid of yolk immunoglobulin antibody is easy to degrade

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Immunogen Preparation

[0051] Standard human rotavirus Wa strain and monkey SA11 strain duplex were used as immunogens, and MA-104 cells were used as host cells for virus culture.

[0052] Take the MA104 cell line from the working cell bank and resuscitate it in a 25ml culture square flask, at 37°C, 5% CO 2, Under the condition of 80% relative humidity, the culture is carried out, and the conventional trypsin digestion method is used for digestion and passage. The culture medium is DMEM medium containing 10% fetal bovine serum. MA104 cells were transferred to a certain amount in the square bottle and then transferred to a 2500ml rotary culture flask for rotary culture at 37°C. After 24 hours, inoculate rotavirus liquid treated with 20ug / ml trypsin at 37°C for 30min, inoculate 5ml of each bottle, and absorb After 1 hour, the virus solution was sucked off, and the maintenance solution (serum-free DMEM medium containing 2ug / ml trypsin) was changed to 37°C for about 7...

Embodiment 2

[0058] 1. Immunogen Preparation

[0059] The standard strain of hepatitis A virus (HAV) was used as the immunogen, and HeLa cells were used as host cells for virus culture.

[0060] Take the HeLa cell line from the working cell bank and resuscitate in a 25ml culture square bottle, at 37°C, 5% CO 2 , Under the condition of 80% relative humidity, the culture is carried out, and the conventional trypsin digestion method is used for digestion and passage. The culture medium is DMEM medium containing 10% fetal bovine serum. Cells are transferred to a certain amount in the square bottle and then transferred to a 2500ml rotary culture bottle for 37°C rotation culture. After 24 hours, inoculate hepatitis A virus liquid, 5ml in each bottle, absorb for 1 hour, and change the maintenance medium (serum-free DMEM culture medium ), 37 DEG C to continue the rotary culture until the cytopathic effect reaches more than 80%, and the virus culture medium is harvested. The virus culture soluti...

Embodiment 3

[0066] 1. Immunogen Preparation

[0067] Ecovirus 2, 4, and 6 triplet viruses were used as immunogens, and human amniotic cells were used as host cells for virus culture.

[0068] Take the passaged human amnion cells from the working cell bank and resuscitate them in a 25ml culture square bottle, at 37°C, 5% CO 2 , Under the condition of 80% relative humidity, the culture is carried out, and the conventional trypsin digestion method is used for digestion and passage. The culture medium is DMEM medium containing 10% fetal bovine serum. Passage human amniotic membrane passage cells to a certain amount in the square bottle, then transfer to a 2500ml rotary culture bottle for rotary culture at 37°C, after 24 hours, inoculate with Echo virus solution, inoculate 5ml in each bottle, absorb for 1 hour, and change the maintenance solution (serum-free DMEM culture medium) at 37° C. for about 72 hours, and the virus culture solution was harvested when the cytopathic effect reached more...

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PUM

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Abstract

The peroral freeze dried specific yolk immune globulin preparation includes specific immune globulin, excipient and solute in solvent. The preparation process includes preparing specific pathogen antigen; adding incomplete Folin reagent in the same amount as the antigen for fully emulsification to obtain immunogen; immunizing the layer with the immunogen; extracting specific yolk immune globulin from the egg of the layer; mixing the specific immune globulin, the excipient and the solvent to obtain mixture liquid; freeze drying the mixture liquid to produce the peroral freeze dried specific yolk immune globulin preparation. The preparation has the specific yolk immune globulin with less denaturation and maintained natural activity, and may be used in preparing medicine, food or health article for treating digestive tract disease caused by pathogenic microbe.

Description

technical field [0001] The invention relates to an immunoglobulin oral freeze-dried preparation and its preparation method and use, in particular to an oral freeze-dried preparation of specific egg yolk immunoglobulin and its preparation method and use. Background technique [0002] There are many diseases caused by pathogenic microorganisms infected through the digestive tract, such as autumn and winter diarrhea in infants and young children caused by rotavirus, polio caused by poliovirus, hepatitis A caused by hepatitis A virus, and Echovirus caused Aseptic meningitis, gastritis caused by Helicobacter pylori, diarrhea caused by Shigella, etc. At present, antibiotics are often used for bacterial diseases, and long-term use in large quantities is likely to produce drug resistance, and antibiotics are ineffective against viruses, mycoplasma, and chlamydia; and for viral diseases, there is currently a lack of specific drugs. And most of the antibiotics and antiviral chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K39/395A61P1/04A61P31/04A61P31/12
Inventor 朱斌史镇钦
Owner SHENZHEN ANTUOXING BIOTECH DEV
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