Oral freeze dried formulation of specific yelk immunoglobulin and its preparation method and usage
A technology of egg yolk immunoglobulin and immunoglobulin, which is applied in freeze-dried transportation, antiviral agents, digestive system, etc., can solve the problems of easy degradation of egg yolk immunoglobulin antibody oral liquid and destruction of antibody activity, and achieve cost reduction, Loss reduction and low cost effects
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Embodiment 1
[0050] 1. Immunogen Preparation
[0051] Standard human rotavirus Wa strain and monkey SA11 strain duplex were used as immunogens, and MA-104 cells were used as host cells for virus culture.
[0052] Take the MA104 cell line from the working cell bank and resuscitate it in a 25ml culture square flask, at 37°C, 5% CO 2, Under the condition of 80% relative humidity, the culture is carried out, and the conventional trypsin digestion method is used for digestion and passage. The culture medium is DMEM medium containing 10% fetal bovine serum. MA104 cells were transferred to a certain amount in the square bottle and then transferred to a 2500ml rotary culture flask for rotary culture at 37°C. After 24 hours, inoculate rotavirus liquid treated with 20ug / ml trypsin at 37°C for 30min, inoculate 5ml of each bottle, and absorb After 1 hour, the virus solution was sucked off, and the maintenance solution (serum-free DMEM medium containing 2ug / ml trypsin) was changed to 37°C for about 7...
Embodiment 2
[0058] 1. Immunogen Preparation
[0059] The standard strain of hepatitis A virus (HAV) was used as the immunogen, and HeLa cells were used as host cells for virus culture.
[0060] Take the HeLa cell line from the working cell bank and resuscitate in a 25ml culture square bottle, at 37°C, 5% CO 2 , Under the condition of 80% relative humidity, the culture is carried out, and the conventional trypsin digestion method is used for digestion and passage. The culture medium is DMEM medium containing 10% fetal bovine serum. Cells are transferred to a certain amount in the square bottle and then transferred to a 2500ml rotary culture bottle for 37°C rotation culture. After 24 hours, inoculate hepatitis A virus liquid, 5ml in each bottle, absorb for 1 hour, and change the maintenance medium (serum-free DMEM culture medium ), 37 DEG C to continue the rotary culture until the cytopathic effect reaches more than 80%, and the virus culture medium is harvested. The virus culture soluti...
Embodiment 3
[0066] 1. Immunogen Preparation
[0067] Ecovirus 2, 4, and 6 triplet viruses were used as immunogens, and human amniotic cells were used as host cells for virus culture.
[0068] Take the passaged human amnion cells from the working cell bank and resuscitate them in a 25ml culture square bottle, at 37°C, 5% CO 2 , Under the condition of 80% relative humidity, the culture is carried out, and the conventional trypsin digestion method is used for digestion and passage. The culture medium is DMEM medium containing 10% fetal bovine serum. Passage human amniotic membrane passage cells to a certain amount in the square bottle, then transfer to a 2500ml rotary culture bottle for rotary culture at 37°C, after 24 hours, inoculate with Echo virus solution, inoculate 5ml in each bottle, absorb for 1 hour, and change the maintenance solution (serum-free DMEM culture medium) at 37° C. for about 72 hours, and the virus culture solution was harvested when the cytopathic effect reached more...
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