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Fungus strain and its application

A technology of bacteria strains and fungi, applied in the direction of fungi, applications, animal repellents, etc., can solve the problem of volatile gases without aroma, achieve the effect of repairing cells, inhibiting the growth of pathogenic bacteria, and high application value

Inactive Publication Date: 2005-11-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the volatile gas produced by Muscodor albus has a strong lethal effect on some pathogenic bacteria, its volatile gas has no aromaticity

Method used

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  • Fungus strain and its application
  • Fungus strain and its application
  • Fungus strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, the isolation culture of bacterial strain:

[0018] The three-line Fusarium of the present invention can be obtained by isolating and culturing in the laboratory according to the following conditions. Collect the bark and branches of the southern yew (Taxus mairei) from the Guanshan Nature Reserve in Jiangxi, my country, disinfect the bark and branches of the collected southern yew on the surface of 5% sodium hypochlorite for 2 minutes, rinse with sterile water and place on sterile filter paper Blot up the water, remove the outer cortex with a sterile blade, cut it into tissue pieces about 1cm in size, and transfer the tissue pieces in 2% containing 100μg·ml -1 Ampicillin and streptomycin on the water agar plate, cultured at 24 ° C, after the growth of the mycelium, the top of the mycelium was transplanted onto the PDA medium, and the purification was repeated 3 times to obtain a pure culture.

Embodiment 2

[0019] Embodiment 2, the antibacterial bioassay of volatile aromatic gas:

[0020] Potato dextrose agar medium (potato dextrose agar, PDA): potato 200g, glucose 20g, agar 20g, water 1000ml.

[0021] Four plant pathogenic fungal strains including Sclerotium rolfsii Saccardo, Rhizoctonia solani Kuhn, Fusarium oxysporum Schlecht. and Botrytiscinerea were selected as indicator strains. Add pre-melted PDA medium to a 9 cm diameter petri dish to make a plate, and use a sterile blade to dig out the 1.0 cm agar strip in the center of the plate. Use a puncher to punch the three-line Fusarium strain and the indicator strain into a mycelium cake with a diameter of 0.4cm, inoculate the three-line Fusarium mycelium cake on one side of the plate, and inoculate the indicator fungus mycelium cake on the plate after culturing at 24°C for 1 week On the other side, no third-line Fusarium was used as a control. The petri dish was wrapped with Parafilm and cultured at 24°C, and the inhibitory ef...

Embodiment 3

[0022] Embodiment 3, volatile gas collection and component analysis:

[0023] The three-line Fusarium strain F0998 was activated and cultivated in PDA medium for later use. Inoculate the mycelium block (diameter 4cm) of the three-line Fusarium strain F0998 that was activated into a 250ml Erlenmeyer flask containing 100ml of PDA medium, culture at 24°C for 1 week, wrap the mouth of the Erlenmeyer flask with parafilm, and continue to cultivate at 24°C for 1 week .

[0024] Insert the solid-phase microextraction device into the Erlenmeyer flask to be tested, dip the extraction head into the Erlenmeyer flask after sticking out the stainless steel protection needle, but do not touch the mycelium, and continue the extraction for 20 minutes. After the extraction balance is reached, the extraction head is retracted into the protective needle tube, immediately inserted into the prepared GC-MS sampling chamber, and the extraction head is pushed out, and the target analyte is analyzed b...

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Abstract

A fungus strain Fusarium tricinctum-ZJUF0998 (CGMCC No.1331), belnoging to Fusarium, Nectriaceae, Hypocreales, Sordariomycetidae, Ascomycetes, Ascomycota, fungi, and the complete sequence of its internal transcription spacer rebosomal DNA are disclosed. It can generate the volatile aromatic gas to suppress the growth of plant pathogenic bacteria.

Description

technical field [0001] The invention relates to a fungus and its application for resisting plant pathogenic fungi, belonging to the field of microbes and microbe applications. Background technique [0002] Many fungi emit low concentrations of gaseous substances, many of which have unpleasant odors. The volatile gas substances produced by some fungi have antibacterial effects. For example, the volatile substances produced by Trichoderma can inhibit the growth of Rhizoctonia solani, Fusarium oxysporum, Pythium ultimum, etc. . At present, no in-depth research has been conducted on the volatile gases produced by fungi. It is generally believed that one of the components of volatile gases is acetaldehyde. Muscodor albus is a new endophytic fungus isolated from Cinnamomum zeylanicum, a Cinnamomum genus plant. Studies have found that this endophytic fungus can produce antibacterial volatile oil components, including 27 components, of which , the main components are ethyl 3-meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 林福呈章初龙陈绍瑗
Owner ZHEJIANG UNIV
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