2, 4-dichlorophen artificial antigen, and its preparing method and use

Abstract

The invention relates to a preparation method and use for 2, 4-di-chlorophenol artificial antigen. It is synthesized by hapten 2, 4-dichlor-6-(4-hydroxyl phenyl azo) phenyl hydroxide through activating fat or admixing acid anhydride. The antigen can be used to make 2, 4-di-chlorophenol antibody, set 2, 4-di-chlorophenol indirect competing fluorescence immunity method, or testing its residuum in the aquatic environment. The advantages of the invention are that the antibody has great practicability and good stability; the preparation method is easy and feasible; the cost is low; and it is easy to produce in industrial scale.

Description

technical field

[0001] The invention belongs to the field of immunochemistry and residue analysis biotechnology of small molecular organic pollutants. It involves organic synthesis, immunochemistry, biochemistry and physical and chemical testing techniques, etc. Specifically, it is a 2,4-dichlorophenol artificial antigen, its preparation method and its use. In particular, it involves the design and synthesis of 2,4-dichlorophenol hapten and artificial whole antigen of environmental hormones, the preparation method of specific antibody for immunized animals, and the establishment of fluorescence that can be applied to the detection of residual 2,4-dichlorophenol in water. Immunoassay techniques. Background technique

[0002] Environmental hormones are hormone analogs in the environment, which can interfere with normal physiological metabolism, endocrine and reproductive functions by binding to hormone receptors, causing various negative biological effects. In the past 70 y...

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