Separation device for solid fermented fungus spore and separation method

A solid-state fermentation, fungal spore technology, applied in fungi, biochemical equipment and methods, bioreactor/fermenter combinations, etc., can solve the problems of high filtration resistance, high labor intensity, and dead ends of stirring, and achieve separation speed. Fast, labor-intensive, and small footprint

Inactive Publication Date: 2006-07-12
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of sieve separation are that, during sieving, the labor intensity is high and there are many impurities; during the centrifugal separation, because the spores and the medium particles are not completely separated during the separation process, the efficiency is low; the filtration separation caused by the filter material has a large filtration resistance
None of the above three methods can well meet the separation requirements of fungal spores
[0004] The fungal spore separation device of Chinese Patent No. 87107268 uses filter material in the gas-powder separation part, and has a complex internal structure, large gas-solid two-phase flow resistance, and high power consumption
[0005] The fungal spore separator of Chinese Patent No. 01275732.2 adopts the following method: the solid medium particles are pulverized in the separation chamber, and the spores suspended above the separation chamber are introduced into the cyclone separator with a circulating air flow. This equipment is prone to dead corners of stirring and feeding The problem of high energy consumption for stirring when the amount is large
[0006] Relevant foreign data show that the solid-state fermentation medium particles are separated from the spores on the surface by using a fluidized bed, and then the spores are introduced into multiple parallel cyclone separators by air flow to improve work efficiency, but the escaping spores will pollute the working environment

Method used

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  • Separation device for solid fermented fungus spore and separation method
  • Separation device for solid fermented fungus spore and separation method
  • Separation device for solid fermented fungus spore and separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The Trichoderma viridans solid-state fermentation medium after weighing 1kg is dried, (moisture content 10%), drop in the feed hopper 2, close feed port 3, start agitator 4, stirring speed is 1500 rev / mins, starts centrifugation after 7 minutes Fan 1, turn on the fan, adjust the valve 14, and use the valve to adjust the air flow rate to 25 m / s. At this time, the solid particles enter the acceleration tube 7 with the air flow, and the accelerated solid particles hit the inertial separator at a speed of 18-20 m / s. 5. The conical disk 17 at the bottom, through high-speed impact, the spores attached to the surface of the solid particles break away and enter the cyclone separator 9 with the air flow, while the solid particles themselves slide down the conical disk 17 to the return pipe 6, and then return to the cyclone separator 9. In the hopper 2, after 10 minutes, all the solid medium particles in the hopper 2 circulate 6 times in the hopper 2, the acceleration tube 7, the ...

Embodiment 2

[0029] The Trichoderma viridans solid-state fermentation medium after weighing 2kg is dried, (moisture content 7%), drop into in the feed hopper 2, close feed inlet 3, start agitator 4, stirring speed is 2000 rev / mins, starts centrifugation after 5 minutes Fan 1, adjust the air flow rate to 25 m / s with valve 14, after 6 minutes, all the solid medium particles in the hopper 2 are in the hopper 2, the acceleration tube 7, the inertial separator 5, the return pipe 6, and the hopper 2 Middle circulation flows 5 times, close fan 1, agitator 4; Can collect the green Trichoderma spore powder of about 75g at the bottom of cyclone separator 9, open discharge port 10, can see that the culture medium of original green becomes khaki.

Embodiment 3

[0031] The Trichoderma viridans solid-state fermentation medium after weighing 1.5kg drying, (moisture content 5%), drop in the feed hopper 2, close feed port 3, start agitator 4, stirring speed is 3000 rev / mins, starts after 3 minutes Centrifugal fan 1, adjust the air flow rate to 25 m / s with a valve, after 5 minutes, all the solid medium particles in the feeding hopper 2 are in the feeding hopper 2, accelerating tube 7, inertial separator 5, return pipe 6, and feeding hopper 2 Middle circulation flows 5 times, close fan 1, mixer 4; Can collect the green trichoderma spore powder of about 40g at the bottom of cyclone separator 9, open discharge port 10, can see that the former green culture medium becomes khaki.

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Abstract

The invention relates to a solid phase enzyme mold-spores segregator, which comprises a feed hopper with feed inlet and feed outlet, a motor, a stirring machine, a centrifugal blower with adjusting valve, a speed pipe, an inertia separator and a cyclone separator. It is characterized in that it arranges the screen cloth on the top part of the inertia separator and the conical disk on the bottom, it is connected with the feed inlet by the return pipe; the top of the inertia separator is connected with the cyclone separator by pipe; the gas outlet of the cyclone separator is connected with the gas cleaning unit by pipe.

Description

technical field [0001] The invention relates to a solid-state fermentation fungal spore separation machine and a matching spore preparation method, belonging to the field of biological processing. Background technique [0002] Currently, in order to improve the active ingredients of biopesticides, it is necessary to efficiently isolate fungal spores. The fungal spores produced by solid-state fermentation adhere to the surface of the medium. Due to the small particle size, the fungal spores are easily suspended in the air during the separation process and are difficult to settle and collect. Therefore, it is necessary to separate and collect the spores by centrifugal field under airtight conditions. [0003] The existing separation technologies for solid particulate materials mainly include: screen separation, centrifugal separation, and filtration separation. The disadvantages of sieve separation are that, during sieving, the labor intensity is high and there are many impur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N1/14
Inventor 纪明山张良谷祖敏王英姿祁之秋张扬
Owner SHENYANG AGRI UNIV
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