Method for detecting Ennoxacin and its special enzyme-linked immune reagent kit
An enzyme-linked immunosorbent reagent, enrofloxacin technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome processes, unsuitable for on-site monitoring and screening of a large number of samples, complex instruments and equipment, etc., and achieve convenient use , high efficiency and high sensitivity
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Embodiment 1
[0031] Example 1. Preparation and detection method of ELISA kit with anti-antibody as coating source
[0032] ELISA kits with anti-antibody conjugates as the coating source include:
[0033] (1) A microtiter plate coated with goat anti-mouse anti-antibody;
[0034] (2) Alkaline phosphatase-labeled enrofloxacin hapten working solution (alkaline phosphatase-labeled enrofloxacin hapten diluted with an aqueous solution containing 0.05% glycerol), 8ml / bottle, 1 bottle.
[0035](3) Enrofloxacin standard solution: 6 bottles of enrofloxacin series standard solutions, 0μg / L, 0.5μg / L, 1.5μg / L, 4.5μg / L, 13.5μg / L, 40.5μg / L, 3ml / bottle. The enrofloxacin drug diluent used is a phosphate buffer solution containing 0.1-0.5% N'N-dimethylformamide (DMF).
[0036] (4) The chromogenic solution is 4-nitrophenol phosphate buffer solution, 8ml / bottle, 1 bottle.
[0037] (5) Enrofloxacin mouse monoclonal antibody working solution: Dilute the monoclonal antibody secreted by enrofloxacin monoclonal...
Embodiment 2
[0071] Example 2, the precision, accuracy and shelf life experiments of the enrofloxacin ELISA kit
[0072] 1. Standard solution precision test
[0073] Get three batches of 02, 06, and 10 from the ELISA kit prepared in Example 1, get 10 kits from each of the three batches, and extract 20 microwells from each microplate, measure 4.0 Calculate the coefficient of variation from the absorbance value (OD value) of the μg / L standard solution. This method was used to examine the intra-panel precision. The measurement results are shown in Table 1. The results showed that, for the three batches of kits, the measurement range of the standard precision was between 4.80% and 9.11%, so the determined precision range was all less than 25%.
[0074] Table 1 Standard repeatability test (CV%)
[0075]
[0076] 2. Sample repeatability test
[0077]Each sample was added with enrofloxacin standard at a concentration of 10 μg / kg, and three different batches of kits prepared in Example 1 w...
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