Process for extracting protohemin by using surfactant

Abstract

This invention is the extraction of chlorhematin with a kind of surface activator. In this invention, water, acid and non-ionic surface active agent or cationic surfactant is added in to hemoglobin from demostic animal's blood to form a blend. Stir it while heat gradually. Stop heat until crystal appear, but continue stiring, cool and centrifuge to isolate the chlorhematin crystal. Then, wash the crystal with water, dry to obtain the product chlorhematin. This method is advantageous in stable reaction, easy manipulation, safe process, low-level cost and good repeatability with plenty of experimental proof.

Description

technical field

[0001] The invention relates to a method for extracting hemin from livestock and poultry blood by using a surfactant. Background technique

[0002] There are many traditional heme manufacturing methods, but they are not ideal all the time. There are many patent reports at home and abroad through literature search, most of which technologies use harmful or flammable and explosive solvents. Some technology products have very low heme content. Some technologies have no treatment method for blood components after hemoglobin extraction to cause pollution.

[0003] Use organic solvent glacial acetic acid method, ethanol / methanol method, ethanol method, glycerol method, ethylene glycol method, dimethylformamide method, methyl ethyl ketone method or acetone method to prepare hemin chloride, which is difficult to recover in large amounts. Therefore, the production cost is high. Among the above-mentioned organic solvents, except glycerol and ethy...

Examples

Embodiment 1

[0012] Add 840ml of water to 100ml of hemoglobin solution (containing 15.2g of dried hemoglobin), add 8g of non-ionic surfactant fatty alcohol polyoxyethylene ether, dissolve in 200ml of water, add 10ml of hydrochloric acid, and gradually heat to 70°C under stirring until crystals appear and the liquid The color becomes blue-purple, and the foam is snow-white. Stop heating and continue to stir for 30 minutes. After standing still and cooling, centrifuge to separate the hemin crystals, then wash the crystals with water, repeat the washing for three times, and dry to obtain the product hemin 0.5319 g, content 90%, yield is 86.34%.

[0013] Add sodium carbonate to the separated liquid for neutralization reaction to produce flocculent protein, filter the protein with a 400-mesh screen, discharge the filtrate, wash the protein with water, filter the screen, and dry to obtain protein powder.

Embodiment 2

[0015] Add 1068ml of water to 100ml of hemoglobin solution (containing 15.2g of dried hemoglobin), add 8g of non-ionic surfactant fatty alcohol polyoxyethylene ether, dissolve it in 200ml of water, add 10ml of hydrochloric acid, and gradually heat to 80°C under stirring until crystals appear and the liquid The color becomes blue-purple, the froth is white, stop heating, continue to stir for 30 minutes, after static cooling, centrifuge to separate the hemin crystals, then wash the crystals with water, repeat the washing for three times, and dry to obtain the product hemin 0.5173 g, content 94.57%, yield 88.34%.

[0016] Add sodium carbonate to the separated liquid for neutralization reaction to produce flocculent protein, filter the protein with a 400-mesh screen, discharge the filtrate, wash the protein with water, filter the screen, and dry to obtain protein powder.

Embodiment 3

[0018] Add 1064ml of water to 100ml of hemoglobin solution (including 15.2g of dried hemoglobin), add 8g of non-ionic surfactant fatty alcohol polyoxyethylene ether, dissolve it in 200ml of water, add 14ml of hydrochloric acid, and gradually heat to 78°C under stirring until crystals appear and the liquid The color becomes blue-purple, and the foam is snow-white. Stop heating and continue to stir for 30 minutes. After static cooling, centrifuge to separate the hemin crystals, then wash the crystals with water, repeat the washing for three times, and dry to obtain the product hemin 0.5340 g, content 92.82%, yield 88.34%.

[0019] Add sodium carbonate to the separated liquid for neutralization reaction to produce flocculent protein, filter the protein with a 400-mesh screen, discharge the filtrate, wash the protein with water, filter the screen, and dry to obtain protein powder.

technical field

[0001] The invention relates to a method for extracting hemin from livestock and poultry blood by using a surfactant. Background technique

[0002] There are many traditional heme manufacturing methods, but they are not ideal all the time. There are many patent reports at home and abroad through literature search, most of which technologies use harmful or flammable and explosive solvents. Some technology products have very low heme content. Some technologies have no treatment method for blood components after hemoglobin extraction to cause pollution.

[0003] Use organic solvent glacial acetic acid method, ethanol / methanol method, ethanol method, glycerol method, ethylene glycol method, dimethylformamide method, methyl ethyl ketone method or acetone method to prepare hemin chloride, which is difficult to recover in large amounts. Therefore, the production cost is high. Among the above-mentioned organic solvents, except glycerol and ethy...

Examples

Embodiment 1

[0012] Add 840ml of water to 100ml of hemoglobin solution (containing 15.2g of dried hemoglobin), add 8g of non-ionic surfactant fatty alcohol polyoxyethylene ether, dissolve in 200ml of water, add 10ml of hydrochloric acid, and gradually heat to 70°C under stirring until crystals appear and the liquid The color becomes blue-purple, and the foam is snow-white. Stop heating and continue to stir for 30 minutes. After standing still and cooling, centrifuge to separate the hemin crystals, then wash the crystals with water, repeat the washing for three times, and dry to obtain the product hemin 0.5319 g, content 90%, yield is 86.34%.

[0013] Add sodium carbonate to the separated liquid for neutralization reaction to produce flocculent protein, filter the protein with a 400-mesh screen, discharge the filtrate, wash the protein with water, filter the screen, and dry to obtain protein powder.

Embodiment 2

[0015] Add 1068ml of water to 100ml of hemoglobin solution (containing 15.2g of dried hemoglobin), add 8g of non-ionic surfactant fatty alcohol polyoxyethylene ether, dissolve it in 200ml of water, add 10ml of hydrochloric acid, and gradually heat to 80°C under stirring until crystals appear and the liquid The color becomes blue-purple, the froth is white, stop heating, continue to stir for 30 minutes, after static cooling, centrifuge to separate the hemin crystals, then wash the crystals with water, repeat the washing for three times, and dry to obtain the product hemin 0.5173 g, content 94.57%, yield 88.34%.

[0016] Add sodium carbonate to the separated liquid for neutralization reaction to produce flocculent protein, filter the protein with a 400-mesh screen, discharge the filtrate, wash the protein with water, filter the screen, and dry to obtain protein powder.

Embodiment 3

[0018] Add 1064ml of water to 100ml of hemoglobin solution (including 15.2g of dried hemoglobin), add 8g of non-ionic surfactant fatty alcohol polyoxyethylene ether, dissolve it in 200ml of water, add 14ml of hydrochloric acid, and gradually heat to 78°C under stirring until crystals appear and the liquid The color becomes blue-purple, and the foam is snow-white. Stop heating and continue to stir for 30 minutes. After static cooling, centrifuge to separate the hemin crystals, then wash the crystals with water, repeat the washing for three times, and dry to obtain the product hemin 0.5340 g, content 92.82%, yield 88.34%.

[0019] Add sodium carbonate to the separated liquid for neutralization reaction to produce flocculent protein, filter the protein with a 400-mesh screen, discharge the filtrate, wash the protein with water, filter the screen, and dry to obtain protein powder.