Process for extracting protohemin by using surfactant

Abstract

This invention is the extraction of chlorhematin with a kind of surface activator. In this invention, water, acid and non-ionic surface active agent or cationic surfactant is added in to hemoglobin from demostic animal's blood to form a blend. Stir it while heat gradually. Stop heat until crystal appear, but continue stiring, cool and centrifuge to isolate the chlorhematin crystal. Then, wash the crystal with water, dry to obtain the product chlorhematin. This method is advantageous in stable reaction, easy manipulation, safe process, low-level cost and good repeatability with plenty of experimental proof.

Description

technical field

[0001] The invention relates to a method for extracting hemin from livestock and poultry blood by using a surfactant. Background technique

[0002] There are many traditional heme manufacturing methods, but they are not ideal all the time. There are many patent reports at home and abroad through literature search, most of which technologies use harmful or flammable and explosive solvents. Some technology products have very low heme content. Some technologies have no treatment method for blood components after hemoglobin extraction to cause pollution.

[0003] Use organic solvent glacial acetic acid method, ethanol / methanol method, ethanol method, glycerol method, ethylene glycol method, dimethylformamide method, methyl ethyl ketone method or acetone method to prepare hemin chloride, which is difficult to recover in large amounts. Therefore, the production cost is high. Among the above-mentioned organic solvents, except glycerol and ethy...