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Hydrogel material for repairing center nerve and preparing method therefor

A central nervous system and hydrogel technology, which is applied in the field of novel central tissue engineering framework materials and hydrogel materials, can solve problems such as unsatisfactory repair effects, and achieve suitable for large-scale production, low cost, and good biocompatibility Effect

Inactive Publication Date: 2006-10-25
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional brain defect repair materials use acrylonitrile and vinyl chloride copolymer, polycarbonate, nitrocellulose, poly-alpha hydroxy acid fruit acid or silicone resin, water-soluble gel polyethylene glycol, N-(2-hydroxypropyl) Methacrylamide (HPMA), these materials promote the regeneration of axons to varying degrees, in addition, there are reports of self-made biological scaffolds formed by lysine, alanine, and aspartic acid, which can promote various The characteristics of adhesion, differentiation and extensive growth of nerve cells, and can promote the formation of synapses between neuron cells, but the repair effect of these materials is not ideal

Method used

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  • Hydrogel material for repairing center nerve and preparing method therefor
  • Hydrogel material for repairing center nerve and preparing method therefor
  • Hydrogel material for repairing center nerve and preparing method therefor

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0029] Example 1: Hyaluronic acid-polylysine hydrogel

[0030] A mixed solution of hyaluronic acid and D-polylysine (PDL) was cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), after cross-linking The product was freeze-dried to form a film.

[0031] The HA-PDL composite matrix prepared by EDC cross-linking combined with freeze-drying technology has a porous network scaffold structure, and has good swelling and mechanical stability.

[0032] Concrete preparation steps are as follows:

[0033] (1) Disperse PDL in distilled water at 4°C, add 1 wt% HA solution to make the ratio of PDL to HA in the mixed solution 0.1, and stir for 30 minutes with a magnetic stirrer.

[0034] (2) The final slurry was poured into a glass dish, covered with parafilm, and freeze-dried at -20°C.

[0035] (3) The prepared porous membrane was immersed in 95% ethanol solution of EDC to react for 24 hours at room temperature.

[0036] (4) The cross-linked membrane was...

Embodiment 2

[0040] Embodiment 2: hyaluronic acid-collagen (type I) hydrogel

[0041] The preparation process of this example is similar to that of Example 1, except that polylysine is replaced with type I collagen.

[0042] (1) Disperse type I collagen in distilled water at 4°C, add 3 wt% hyaluronic acid solution to make the ratio of collagen to HA in the mixed solution 1, and stir for 30 minutes with a magnetic stirrer.

[0043] (2) The final slurry was poured into a glass dish, covered with parafilm, and freeze-dried at -20°C.

[0044] (3) The prepared porous membrane was immersed in 95% ethanol solution of EDC to react for 24 hours at room temperature.

[0045] (4) The cross-linked membrane was ultrasonically cleaned in distilled water for 1 hour to remove residual EDC, and then freeze-dried at -20°C after cleaning.

[0046] Thus, a hyaluronic acid-collagen composite hydrogel modified by EDC cross-linking is obtained. figure 2 It is the SEM photograph of the collagen-HA hydrogel in...

Embodiment 3

[0047] Embodiment 3: hyaluronic acid-polylysine hydrogel

[0048] The preparation process of this example is similar to Example 1, except that the ratio of PDL and HA is 0.5.

[0049] (1) Disperse PDL in distilled water at 4° C., add 1 wt % hyaluronic acid solution to make the ratio of PDL to HA in the mixed solution 0.5, and stir for 30 minutes with a magnetic stirrer.

[0050] (2) The final slurry was poured into a glass dish, covered with parafilm, and freeze-dried at -20°C.

[0051] (3) The prepared porous membrane was immersed in 95% ethanol solution of EDC to react for 24 hours at room temperature.

[0052] (4) The cross-linked membrane was ultrasonically cleaned in distilled water for 1 hour to remove residual EDC, and then freeze-dried at -20°C after cleaning.

[0053] Thus, a hyaluronic acid-polylysine composite hydrogel modified by EDC cross-linking is obtained.

[0054] image 3 It is the SEM photo of the PDL-HA hydrogel in Example 3 (w / w=0.5).

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Abstract

The present invention relates to a hydrogel material for repairing central nerve and its preparation method. Said method includes the following steps: using hyaluronic acid as main component, making it and one or several high-molecules implement copolymerization reaction to obtain porous frame material, preparing preformed material, mixing them and freeze-drying, covalently cross-linking, ultrasonic cleaning and freeze-drying so as to the invented hydrogel frame material. In the hydrogel frame material one or several kinds of substances of growing factor, cell and protein can be added.

Description

technical field [0001] The present invention relates to a hydrogel material, in particular to a hydrogel material used in the repair of the central nervous system for promoting nerve regeneration, slow release of cells, proteins, etc., and a preparation method thereof, which is a novel A central tissue engineering frame material belongs to the field of central tissue engineering frame material and preparation technology. Background technique [0002] Tissue engineering scaffold materials play the following roles in nerve regeneration: inhibiting the formation of glial scars; providing bridges for the growth of blood vessels and nerve axons; serving as carriers of growth factors and replacing cells. Traditional brain defect repair materials use acrylonitrile and vinyl chloride copolymer, polycarbonate, nitrocellulose, poly-alpha hydroxy acid fruit acid or silicone resin, water-soluble gel polyethylene glycol, N-(2-hydroxypropyl) Methacrylamide (HPMA), these materials promote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/20A61L31/04
Inventor 崔福斋田维明徐群渊侯少平魏岳腾
Owner TSINGHUA UNIV