Method for preparing artificial skin used for reparing skin defect

A technology for artificial skin and skin repair, applied in skin transplantation, biochemical equipment and methods, artificial cell constructs, etc., can solve the problems of natural skin structure differences, lack of clinical application, and inability to form skin basement membrane structure, etc., to achieve evenly distributed effect

Inactive Publication Date: 2006-11-29
陕西艾尔肤组织工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prepared artificial skin has good elasticity and flexibility, and can be cut, but there are still some defects: (1) the composition of the matrix gel is complex, which increases the cost of clinical application; (2) the source of the cells in the artificial skin is not clear, The application and use of the obtained artificial skin are unknown; (3) Our research proves that the in vitro culture conditions of epidermal cells and fibroblasts are very different, and the culture medium used in this patent is not suitable for the common growth of epidermal cells and fibroblasts. Cultivation will directly affect the growth of epidermal cells and fibroblasts, resulting in that the texture and structure of artificial skin cannot meet the needs of clinical use; (4) no medium that is conducive to keratinization of epidermal cells is used, so that the cultured artificial skin has no stratum corneum, There are differences from natural skin; (5) There is no detailed protocol for in vitro culture of artificial skin in the literature, and technicians cannot repeat it
From literature, the deficiency of this invention is: (1) this invention does not establish three-dimensional culture environment, and fibroblast can not grow in the collagen sponge membrane but only grows on its surface, thereby does not form real dermal tissue; (2) In th

Method used

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  • Method for preparing artificial skin used for reparing skin defect
  • Method for preparing artificial skin used for reparing skin defect
  • Method for preparing artificial skin used for reparing skin defect

Examples

Experimental program
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Embodiment Construction

[0039] 1. Take the skin tissue after circumcision of adolescents, digest it with Dispase, digest it at 37°C for 80 minutes, separate the epidermis and dermis tissue, and digest the epidermis tissue with trypsin at 37°C for 5 minutes to obtain epidermal cells. The dermal tissue was digested with collagenase, and fibroblasts were obtained after digestion at 37°C for 120 minutes.

[0040] 2. Preparation of artificial skin culture solution

[0041] 1.) Preparation of basal culture medium

[0042] Ingredient Final Concentration

[0043] Commercial Minimum Essential Medium (DMEM) 67.5%

[0044] Commercial culture medium F12 22.5%

[0045] Fetal Bovine Serum 10%

[0046] Insulin 40ng / ml

[0047] Hydrocortisone 800ng / ml

[0048] Basic Fibroblast Growth Factor 6ng / ml

[0049] Transferrin 2mg / ml

[0050] Adenine 0.1mM

[0051] Antibiotics 800i.u / ml

[0052] 2.) Preparation of artificial skin culture solution:

[0053] Culture medium A: basal culture medium + epidermal growth ...

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Abstract

An artificial skin for repairing the skin defect caused by inflammation, ulcer, burn, etc is composed of surface epidermal cells and fibroblasts. It is prepared through preparing culture liquid, preparing the solution of artificial skin matrix, and culturing artificial skin.

Description

technical field [0001] The invention belongs to the technical field of artificial organ tissue engineering, and relates to a method for preparing artificial skin for repairing skin defects. Background technique [0002] Skin is an important tissue organ that covers and protects the body surface. Skin defects are often caused by inflammation, ulcers, burns, iatrogenic and other reasons. At present, autologous skin transplantation is often used for such tissue defects. Hide source restrictions. In addition, grafts often differ in color, texture, and function from the skin adjacent to the graft site. An ideal artificial skin substitute should have the following characteristics: structure similar to normal skin tissue, have certain adhesion to the wound, prevent bacterial invasion and growth, be used to repair skin tissue defects, be non-toxic, and not produce immune rejection , and has good biocompatibility, etc. In recent years, the technology of tissue engineering to deve...

Claims

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Application Information

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IPC IPC(8): A61F2/10A61L27/00C12N5/08C12N5/071
Inventor 金岩张勇杰刘源赵宇
Owner 陕西艾尔肤组织工程有限公司
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