Method for preparing artificial skin used for reparing skin defect

Abstract

An artificial skin for repairing the skin defect caused by inflammation, ulcer, burn, etc is composed of surface epidermal cells and fibroblasts. It is prepared through preparing culture liquid, preparing the solution of artificial skin matrix, and culturing artificial skin.

Description

technical field [0001] The invention belongs to the technical field of artificial organ tissue engineering, and relates to a method for preparing artificial skin for repairing skin defects. Background technique [0002] Skin is an important tissue organ that covers and protects the body surface. Skin defects are often caused by inflammation, ulcers, burns, iatrogenic and other reasons. At present, autologous skin transplantation is often used for such tissue defects. Hide source restrictions. In addition, grafts often differ in color, texture, and function from the skin adjacent to the graft site. An ideal artificial skin substitute should have the following characteristics: structure similar to normal skin tissue, have certain adhesion to the wound, prevent bacterial invasion and growth, be used to repair skin tissue defects, be non-toxic, and not produce immune rejection , and has good biocompatibility, etc. In recent years, the technology of tissue engineering to deve...

AI Technical Summary

Problems solved by technology

The prepared artificial skin has good elasticity and flexibility, and can be cut, but there are still some defects: (1) the composition of the matrix gel is complex, which increases the cost of clinical application; (2) the source of the cells in the artificial skin is not clear, The application and use of the obtained artificial skin are unknown; (3) Our research proves that the in vitro culture conditions of epidermal cells and fibroblasts are very different, and the culture medium used in this patent is not suitable for the common growth of epidermal cells and fibroblasts. Cultivation will directly affect the growth of epidermal cells and fibroblasts, resulting in that the texture and structure of artificial skin cannot meet the needs of clinical use; (4) no medium that is conducive to keratinization of epidermal cells is used, so that the cultured artificial skin has no stratum corneum, There are differences from natural skin; (5) There is no detailed protocol for in vitro culture of artificial skin in the literature, and technicians cannot repeat it

From literature, the deficiency of this invention is: (1) this invention does not establish three-dimensional culture environment, and fibroblast can not grow in the collagen sponge membrane but only grows on its surface, thereby does not form real dermal tissue; (2) In this invention, epidermal cells are seeded and cultured on the other side of the porous collagen (a cell-free, non-porous collagen surface), so that the epidermal cells and the fibroblasts in the dermis have no mutual relationship, so that the important basement membrane structure in the skin cannot be formed. It makes the epidermis and dermis very easy to peel off, which affects the effect of transplantation and repair; (3) the skin of this invention cannot stratify the epidermis (the maturation process of the epidermis), which eventually leads to a significant difference between the cultured skin and the natural skin

The disadvantage of this invention is that (1) the dermal scaffold material uses polyglycolic acid (PGA), and the degradation product of this material is acidic, which will affect the healing of the wound; (2) the fibroblasts are planted on the surface of the PGA membrane rather than inside the material, Therefore, it is difficult to grow in a three-dimensional direction under physiological conditions, which is different from the natural skin structure; (3) There is no specific in vitro culture plan, and technicians cannot repeat it

[0008] The above three inventions have no proof or effect description of clinical application or animal experiments, so whether these products can be used clinically is not convincing

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