Process for preparing red alga oligose

Abstract

The present invention discloses process of preparing red algae oligose with acid treated solid cationic exchange resin as medium. The preparation process has the advantages of the repeated use of acid treated solid cationic exchange resin, low cost, no acid liquid exhaust, no corrosion to the production apparatus and less technological steps. The product has carrgeenin oligose with obvious effect of antagonizing pneumonia virus and herpes virus, relatively high effect of inhibiting tumor cell, effect of inhibiting the growth of Bacillus subtilis, etc; and agaragar oligose with high antioxidant and liver protecting effect.

Description

technical field [0001] The invention relates to a method for preparing oligosaccharides, in particular to a method for preparing red algae oligosaccharides. Background technique [0002] Red algae polysaccharides extracted from red algae mainly include agar gum and carrageenan. Red algal polysaccharides have a long history of application in food and medicine, and red algal oligosaccharides are low-molecular-weight compounds obtained after polysaccharide degradation. In recent years, many physiological activities of this type of oligosaccharides and their derivatives have been gradually recognized. Oligosaccharides have been reported in anti-oxidation, anti-inflammation, hypoglycemic and anti-tumor aspects; and carrageenan oligosaccharides are a kind of natural polyanionic sulfate oligosaccharides, so they have anti-tumor, antibacterial, anticoagulant and other aspects. Its unique activity, in addition, as an antiviral active substance, carrageenan oligosaccharide has a good...

AI Technical Summary

Problems solved by technology

Red algal polysaccharides have a long history of application in food and medicine, and red algal oligosaccharides are low-molecular-weight compounds obtained after polysaccharide degradation. In recent years, many physiological activities of this type of oligosaccharides and their derivatives have been gradually recognized. Oligosaccharides have been reported in anti-oxidation, anti-inflammation, hypoglycemic and anti-tumor aspects; and carrageenan oligosaccharides are a kind of natural polyanionic sulfate oligosaccharides, so they have anti-tumor, antibacterial, anticoagulant and other aspects. Its unique activity, in addition, as an antiviral active substance, carrageenan oligosaccharide has a good inhibitory effect on HIV virus, HSV virus, herpes and hepatitis virus, etc., so its application prospect is good; The degradation methods mainly include enzymatic degradation, liquid acid degradation and oxidative degradation. In the Chinese patent (Patent No. CN1467226), the Fenton oxidative degradation method is adopted. This method causes the oxidation degree of the product to be serious, and there are many by-products, which cause great harm to the subsequent separation and purification. Big difficulty; Japanese patent (patent No. JP2000116376) uses carrageenase to prepare κ-carrageenan oligosaccharide, and Chinese patent (patent No. CN1513989) also uses agarase to degrade agar, but enzymatic hydrolysis first needs to separate Extract the required enzyme, and then degrade it to obtain the oligosaccharide mixture. Because the source of the enzyme is generally marine microorganisms or snails, it is expensive and not suitable for industrial production; Chinese patents (patent No. CN1394879A, CN1513860) use Oxalic acid, hydrochloric acid, sulfuric acid, etc. degrade agar, and in the Chinese patent (CN1513880A), a dilute sulfuric acid solution is used as a hydrolysis medium to produce carrageenan oligosaccharides. If these methods of using liquid acids are used for industrial production, it will cause Equipment corrosion and discharged waste water can easily lead to environmental pollution, and the neutralization step in the method will produce a large amount of salt. Therefore, a desalination process is also required in production, resulting in complex processes, increased production costs, and low production efficiency.