Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Boactivity peptide gene therapy method realized by propeptide recombination

A bioactive peptide and gene therapy technology, applied in gene therapy, peptide preparation methods, chemical instruments and methods, etc., can solve problems such as high cost, failure to meet industrial production requirements, and inability to realize the prevention and treatment of bioactive peptides. To achieve the effect of ensuring identification and effective cutting and preventing degradation

Inactive Publication Date: 2007-02-28
BEIJING TAIHE BIOTECH CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention overcomes the problems existing in the prior art that cannot meet the requirements of industrialized production, is expensive, and cannot be used as a drug to enter the clinical simultaneous expression. Most of the products cannot realize the preventive and therapeutic effects of biologically active peptides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Boactivity peptide gene therapy method realized by propeptide recombination
  • Boactivity peptide gene therapy method realized by propeptide recombination
  • Boactivity peptide gene therapy method realized by propeptide recombination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Construction of recombinant propeptide of anti-tumor angiogenesis peptide Anginex and its gene therapy

[0031] (1) Determine the sequence of the bioactive peptide: XXXXXX in the figure represents the amino acid sequence of the selected bioactive peptide, and each X represents an amino acid residue. According to the desired biological effect, the user selects a peptide with specific biological activity from the published literature or through his own preliminary research findings, such as the anti-tumor effect of promoting apoptosis and inhibiting tumor angiogenesis Peptides, antiviral infection peptides that antagonize HIV or HCV invading target cell receptors, aptamer peptides that block intracellular signal transduction, and nutritional peptides that activate cell functions and treat senile degenerative diseases, etc. The mechanisms and principles of action of these bioactive peptides are diverse, and the subcellular locations where they act are also diver...

Embodiment 2

[0128] Example 2, construction of TAT-VHLβ structural propeptide and anti-kidney cancer effect:

[0129] The first step: determine the sequence of biologically active peptides: Renal cell carcinoma is a common malignant tumor, recurrence and metastasis after surgery are common, and the tumor is not sensitive to radiation and chemical anticancer drug treatment, so the patient's prognosis Difference. Recent biological studies of renal cancer cells have revealed that the proliferation and metastasis of the cells depend on insulin-like growth factor 1 (insulin-like growth factor 1, ILGF1) receptor signaling. Yon Hippel-Lindau (VHL) is a tumor suppressor gene, and its expression product inhibits the ILGF1 receptor signal through the combination with protein kinase Cδ, leading to the apoptosis of renal cancer cells, and has the effect of treating renal cancer. However, there are technical difficulties in using VHL protein to treat renal cancer solid tumors. One is that protein mole...

Embodiment 3

[0178] Example 3, construction of recombinant adeno-associated virus expressing anti-Alzheimer's Humanin propeptide and its protective effect on neurons.

[0179] Alzheimer's disease (AD) is a common degenerative disease of the central nervous system in the elderly. The etiology is currently unknown, but the pathology is characterized by neuronal loss, senile plaque formation, and neurofibrillary tangles. A 24-amino acid residue polypeptide-Humanin was discovered using molecular biology screening techniques, which can antagonize AD pathological changes caused by various known genetic causes, especially neural apoptosis. We used this patented technology to construct a recombinant propeptide expressing Humanin, expressed the propeptide using an adeno-associated virus vector, transduced rat cortical neurons, and successfully prevented amyloid peptide (the most important neuron-promoting apoptosis in AD) Apoptosis induced by dead substances).

[0180] Step 1: Determine the seque...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a gene treating method of biological active peptide through reconstructing front-peptide in the biological technological domain, which comprises the following steps: (1) affirming sequence of biological active peptide, (2) loading identification sequence of signal peptide enzyme at amino end of object peptide, (3) deviating cDNA code according to object peptide sequence, (4) synthesizing cDNA, (5) plugging double-chain cDNA segment with peptide-terminal and interior contact enzyme identification point to prepare expressive box.

Description

Technical field: [0001] The invention relates to the field of biotechnology, in particular to a gene therapy method for realizing biologically active peptides through recombinant propeptides. Background technique: [0002] Bioactive peptides are structural domains and functional region fragments of proteins. Compared with parent protein molecules, they have the characteristics of small molecular weight, simple structure and specific function, and some biological functions of some peptides are greater than their parent proteins. Therefore, in the prevention, It has important application value in diagnosis and treatment of diseases. How to obtain bioactive peptides is an important topic in the research and use of peptides. From the current scientific and technological level, there are several ways to obtain bioactive peptides: [0003] 1. Extract bioactive peptides from animal and plant tissues rich in bioactive peptides: this is also the earliest method to obtain bioactive p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00C07K1/00C07K14/00
Inventor 杨广孝
Owner BEIJING TAIHE BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com