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Preparation method of magnetic surface strength laman scattering mark

A surface-enhanced Raman and Raman scattering technology, used in the fields of nanomaterials and bioanalytical chemistry, can solve the problems of complex preparation methods and difficult control, and achieve the effects of simple operation, good stability and wide excitation spectrum.

Inactive Publication Date: 2009-11-04
SOUTHEAST UNIV
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  • Description
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  • Application Information

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Problems solved by technology

However, the preparation method of the marker is complicated and not easy to control

Method used

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  • Preparation method of magnetic surface strength laman scattering mark
  • Preparation method of magnetic surface strength laman scattering mark

Examples

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Effect test

Embodiment 1

[0032] Example 1, preparation of a magnetic SERS label using rhodamine 6G as a reporter molecule

[0033] The first step is to prepare magnetic nanoparticles with superparamagnetism.

[0034] Magnetic nanoparticles can be prepared by chemical co-precipitation method. This method uses Fe 3+ and Fe 2+ React under alkaline conditions to get Fe 3 o 4 Crystals precipitated. To better disperse the magnetic nanoparticles, surfactants can be added to the medium or the pH value of the aqueous solution can be changed. The particle size of the magnetic nanoparticles is preferably less than 30nm, and most preferably between 5nm and 12nm.

[0035] The second step is to prepare the magnetic SERS substrate.

[0036] Under ice bath conditions, 150mL concentration was 2×10 -3 The sodium borate solution of M was placed in a 500mL flask and kept stirring. Add 20 mL of the magnetic nanoparticle solution obtained in the first step, continue to stir, and mix evenly. Then add 50mL to a con...

Embodiment 2

[0040] Example two, preparation of a magnetic SERS probe using 2-aminophenol sulfur as a reporter molecule

[0041] The specific steps are roughly the same as above, the difference is that in the third step, the concentration is 10 -3 M's 2-aminophenolthiomethanol solution instead of a concentration of 10 -5 M's rhodamine 6G solution.

[0042] Drop the magnetic SERS label on the silicon wafer and fix it on the confocal Raman spectrometer to detect its SERS spectrum, such as figure 2 shown. The laser source is a 488nm argon ion laser, and the irradiation power on the sample is 1.2mW. The integration time is 1 minute. The label has both magnetic properties and a SERS signal with a high signal-to-noise ratio, can be controlled by a simple magnetic field, and is suitable for biological imaging and target molecule detection.

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Abstract

A method for preparing a magnetic surface-enhanced Raman scattering label relates to the fields of nanomaterials and bioanalytical chemistry. The preparation process of the Raman scattering label is as follows: a superparamagnetic metal oxide magnetic nanoparticles; under ice bath conditions, put 120-300mL sodium borate solution with a concentration of 2×10-3M in the flask, and keep stirring; add 20-50mL magnetic nanoparticle solution, continue stirring, and mix Uniform; add 40-100mL silver nitrate solution with a concentration of 5×10-3M, and stir at room temperature for 1-3 hours to obtain a magnetic SERS active substrate; select a chemical molecule with a large Raman scattering cross-section as the SERS reporter molecule; The magnetic SERS active substrate labeled with the SERS reporter molecule is separated from the solution by centrifugation; then 0.1M sodium chloride solution is added at a volume ratio of 3:1 to 10:1 to obtain a magnetic SERS label.

Description

Technical field: [0001] The invention relates to the fields of nanomaterials and bioanalytical chemistry, in particular to a magnetic surface-enhanced Raman scattering label and a preparation method thereof. Background technique: [0002] Magnetic fluorescent nanomaterials are materials with both magnetic and luminescent properties. Due to its magnetic properties, under the action of an external magnetic field, this nanomaterial is easily separated and detected from biomolecules, and has broad application prospects in the field of biomedicine. And because it can quickly gather in a magnetic field environment, it provides the possibility for targeted drug delivery. In recent years, people have paid more attention to the application of magnetic fluorescent materials in biomarkers, and developed magnetic fluorescent labels with various structures to realize the separation and detection of biomolecules or cell imaging. However, such materials face problems such as low fluoresc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N27/72C12Q1/04
Inventor 王著元崔一平吕昌贵恽斌峰
Owner SOUTHEAST UNIV
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