Polysaccharides nano material with antibiotic function, preparing method and application thereof

A technology of nanomaterials and polysaccharides, which is applied in the field of antibacterial functional polysaccharide nanomaterials and its preparation, can solve the problem of high toxicity of crosslinking agents, adverse effects on the integrity of cell macromolecular drugs, and the particle size of copper-loaded chitosan particles. major issues

Inactive Publication Date: 2007-08-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to introduce anionic surfactants such as sodium tripolyphosphate, and the particle size of the obtained copper-loaded chitosan particles is large
[0006] Tanima, b., susmita, m., singh, K. Preparation characterization and biodistribution of ultrafine ehitosan nanopanicles. International Journal of pharm., 2002, 243(1-2): 93-102 reported in -(2. Ethylhexyl) A method of preparing chitosan nanoparticles for protein drug release in a thiosuccinic acid (AOT) / n-hexane reversed-phase micelle system using glutaraldehyde. When the concentration of nanoparticle solution is about 4%, and the degree of cross-linking is 10%, the particle size is about 75nm, and when the degree of cross-linking is 100%, the particle size reaches 500-600nm. Chemical cross-linking often affects the survival of cells. The efficiency and the integrity of macromolecular drugs are adversely affected, and the cross-linking agent is more toxic, so the chitosan drug carrier prepared by this method has certain limitations in application.

Method used

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  • Polysaccharides nano material with antibiotic function, preparing method and application thereof
  • Polysaccharides nano material with antibiotic function, preparing method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Preparation weight concentration is the aqueous solution of 1% carboxymethyl chitosan (molecular weight 100000, degree of substitution 85%, degree of deacetylation 95), under magnetic stirring condition (stirring speed 600rpm) will concentration be the CuSO of 100mmol / L 4 The solution (20ml) was added dropwise to 100ml of a 1% carboxymethyl chitosan solution (pH 4) and the temperature was kept constant at 25° C. at a rate of 300ml / h. Stirring was continued for 30 minutes after the addition was completed. The pH adopts the NaOH solution that weight concentration is 1% to adjust;

[0027] The resulting reaction mixture was centrifuged at 15,000 rpm for 4 h with a centrifuge, and the centrifuged solid was repeatedly washed with deionized water, washed with BaCl 2 Test solution to no SO 4 2- Existence, the obtained solid was dispersed in 100ml of water under the action of ultrasound. The resulting dispersion was dried by spraying (dryer inlet temperature 165° C.) to obta...

Embodiment 2

[0030] Preparation weight concentration is the aqueous solution of 1% carboxymethyl chitosan (molecular weight 100000, degree of substitution 85%, degree of deacetylation 95), under magnetic stirring condition (stirring speed 600rpm) will concentration be the CuSO of 100mmol / L 4 The solution (20ml) was slowly added dropwise to 100ml of a 1% carboxymethyl chitosan solution (pH 4), and the temperature was kept constant at room temperature at a rate of 300ml / h, and continued to stir for 30min after the addition was completed. The pH adopts the NaOH solution that weight concentration is 1% to adjust;

[0031] The resulting reaction mixture was centrifuged at 15,000 rpm for 4 h with a centrifuge, and the centrifuged solid was repeatedly washed with deionized water, washed with BaCl 2 Test solution to no SO 4 2- Existence, the obtained solid was dispersed in 100ml of water under the action of ultrasound. The resulting dispersion was freeze-dried to obtain copper-loaded chitosan-b...

Embodiment 3

[0034] Preparation weight concentration is the solution of 1% carboxymethyl chitosan (molecular weight 500000, degree of substitution 85%, degree of deacetylation 95), under magnetic stirring condition (stirring speed 600rpm) will concentration be the CuSO of 100mmol / L 4 The solution (20ml) was slowly added dropwise to 100ml of a 1% carboxymethyl chitosan solution (pH 4) and the temperature was kept constant at room temperature at a rate of 300ml / h. Stirring was continued for 30min after the addition was complete. The pH adopts the NaOH solution that weight concentration is 1% to adjust;

[0035] The resulting reaction mixture was centrifuged at 15,000 rpm for 4 h with a centrifuge, and the centrifuged solid was repeatedly washed with deionized water, washed with BaCl 2 Test solution to no SO 4 2- Existence, the obtained solid was dispersed in 100ml of water under the action of ultrasound. The resulting dispersion was dried by spraying (dryer inlet temperature 165° C.) to obt...

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Abstract

The invention discloses a polyose nanometer material possessing antibiotic function and preparing method and application, which comprises the following steps: choosing water-soluble polyose material incorporating carboxy group or sulfonic group as basis material; loading metallic ion possessing bacterial inhibition active in water solution; setting metallic ion load capacity at 10-500mg/g and bacterial inhibition active at 99.9%; centrifugal separating; ultrasonic dispersing; freezing or spraying; drying; getting end product with particle diameter at 20-800nm. This invention can be used to textile industry, animal husbandry and medicine domain as antiviral drug.

Description

technical field [0001] The invention relates to a polysaccharide nanomaterial, in particular to a polysaccharide nanomaterial with antibacterial function, which uses a water-soluble polysaccharide substance containing carboxyl or sulfonic acid groups as a basic raw material, and is loaded with antibacterial active metal ions And its preparation method and use. technical background [0002] Polysaccharides such as cellulose, chitosan and alginic acid are the most abundant natural polymers in nature. They have good biocompatibility, non-toxicity, biodegradability and environmental friendliness. They are used in biomedicine, Food, textile and other fields have a wide range of uses. Microspheres and beads of polysaccharides have been developed and used in tissue engineering, anticancer drugs, and protein controlled release systems. [0003] At present, the methods for preparing microspherical polysaccharides include: [0004] Complex coacervation method, covalent cross-linkin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/00A01N31/00A01P1/00A61K31/715A23K1/16A23K20/163
Inventor 孙宾朱美芳陈彦模顾春菊周哲
Owner DONGHUA UNIV
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