Chitosan oligosaccharide/chito-oligomer single enzymatic production process

A production process, chitosan oligosaccharide technology, applied in the field of specific enzymatic production technology of chitosan oligosaccharide/chitosan oligomer, can solve the problems that hinder the production, sales and application of chitosan oligosaccharide, and the molecular weight distribution of chitosan oligosaccharide products Uniformity, inconsistent physiological activity, etc., to achieve the effect of uniform size, relative size, and controllable quality

Active Publication Date: 2007-08-29
泉州市科思生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing enzymatic preparation of chitosan oligosaccharides basically uses non-specific compound enzymes, such as lipase, amylase, lysozyme, proteolytic enzyme, etc., the main disadvantages are: non-specificity, low degradation efficiency , the molecular weight distribution of chitosan oligosaccharide products is not uniform, the physiological activity is inconsistent, and the demand for high-quality chitosan oligosaccharide intermediates in the food and pharmaceutical markets at home and abroad is not met, which seriously hinders the production, sales and application of chitosan oligosaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 3% chitosan is dissolved in the acetic acid of 1% concentration, adjusts pH to 5.8 with saturated sodium bicarbonate solution, adds immobilized endo-chitosan enzyme preparation by 5U / g, and immobilized endo-chitosan enzyme preparation is obtained by Provided by the chitosan resource development and utilization project team of Jiangxi Normal University; the enzymolysis reaction temperature is 45°C, and the enzymolysis reaction time is 3 hours; the immobilized enzyme is recovered by sieve filtration, and the enzymolysis supernatant is obtained; Add 30% column volume of enzymolysis supernatant to the upper end of the weakly acidic cationic resin D155 chromatography column, 0-3mol / L ammonia water gradient elution, collect and use DNS (3,5-dinitrosalicylic acid) reagent to detect whether the eluate is Containing reducing sugar; the white precipitate formed by chitosan components with larger molecular weights that have not been degraded into chitosan oligosaccharides in the el...

Embodiment 2

[0015] 3% chitosan is dissolved in the acetic acid of 1% concentration, adjusts pH to 5.8 with saturated sodium bicarbonate solution, adds the endo-chitosan enzyme preparation of free state by 5U / g, the endo-chitosan enzyme preparation of free state is obtained by Provided by the chitosan resources development and utilization project team of Jiangxi Normal University; the enzymolysis reaction temperature is 45°C, and the enzymolysis reaction time is 3 hours; the free state enzyme is recovered by ultrafiltration membrane filtration, and the enzymolysis supernatant is obtained; Add 30% column volume enzymolysis supernatant to the upper end of macroporous weakly acidic cationic resin D155 chromatographic column, 0-3mol / L ammonia water gradient elution, collect and use DNS reagent to detect whether the eluate contains reducing sugar; The white precipitate formed by chitosan components with larger molecular weights degraded into chitooligosaccharides can be directly filtered and rec...

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PUM

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Abstract

The invention is a chitooligosaccharide / chito oligomer production process by specific enzyme, firstly preparing 1-6% chitosan solution, adding in saturated sodium bicarbonate solution to regulate pH value, adding in endo-chitosanase for enzymic hydrolysis, then adopting macroporous weakly acidic cation resin chromatographic column to separate and purify the degraded product- chitooligosaccharide, and after reaction ends, adopting screen filter method to recover immobilized enzyme, adopting ultrafiltration membrane to recover dissociative enzyme, and able to use repeatedly. And the process can make high efficiency specific degradation on chitosan, extremely raising yield of chitooligosaccharide, having featuring of simple production process, largely reduced production cost, and effectively improved quality of chitooligosaccharide product. And the whole production process is a continous production process, which meets the requirements for industrialized large-scale production of chitooligosaccharide product.

Description

technical field [0001] The invention relates to a specific enzymatic production process of chitosan oligosaccharide / chitos oligomer. Background technique [0002] Oligochitosan is a soluble small molecule chitosan with a degree of polymerization of glucosamine in the range of 2≤n≥10, and a chitosan oligomer is a soluble chitosan with a degree of polymerization n greater than 10. Chitooligosaccharides and chitosan oligomers are the key intermediates of chitosan series applications. They have good water solubility, high biological activity, diverse functions, and are easily absorbed by the human body. They are used in medicine, food, daily chemicals, industry and agriculture, etc. It has a huge application prospect, and is known as the sixth vital element of the human body and the reputation of soft gold at home and abroad. [0003] At present, the preparation of oligochitosan mainly adopts chemical degradation method and non-specific compound enzyme method. Using chemical m...

Claims

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Application Information

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IPC IPC(8): C12P19/04
Inventor 王曼莹游清徽
Owner 泉州市科思生物科技有限公司
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