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Method for measuring gelatine molecular weight distribution in copper electrolysing solution

A molecular weight distribution and measurement method technology, applied in the field of molecular weight distribution testing, can solve the problems of fast degradation of gelatin, increase of equipment, long analysis time, etc., achieve accurate and reliable analysis results, prolong service life, and reduce analysis costs

Inactive Publication Date: 2007-09-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common disadvantage of these methods is that the part with a molecular weight below 20,000 cannot be collected quantitatively
[0004] Because the gelatin in the copper electrolyte is in a strongly acidic condition, conventional GPC analysis faces strong acidity, and the chromatographic column cannot bear it; moreover, the gelatin degradation rate under acidic conditions is fast, the process control is difficult, and the ion interference of the strong electrolyte copper sulfate will also Greatly affects molecular weight determination
Japanese patent 01801130.6 discloses that the method for measuring the molecular weight distribution of gelatin is to adopt ion exchange resin bonded chromatographic column switching technology and high performance liquid chromatography to analyze gelatin molecular weight, but the column chromatography program is complicated, and the column switching technology involves the increase of devices, which increases the cost of analysis and require longer analysis time

Method used

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  • Method for measuring gelatine molecular weight distribution in copper electrolysing solution
  • Method for measuring gelatine molecular weight distribution in copper electrolysing solution
  • Method for measuring gelatine molecular weight distribution in copper electrolysing solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] It is used to determine the molecular weight distribution of gelatin without electrolyte under near-neutral conditions.

[0024] 1. Solid phase extraction technology.

[0025] Including the following steps and process conditions:

[0026] (1) Place the solid-phase extraction cartridge on the solid-phase extraction apparatus, and connect the top of the solid-phase extraction cartridge to a 50mL syringe through an adapter. The extraction column used is the HLB solid phase extraction column of Waters Company, the specification is 3cc60mg.

[0027] (2) First use 1mL of methanol, then activate the extraction cartridge with 1mL of water;

[0028] (3) Inject 150 mL of simulated sample containing gelatin aqueous solution from the syringe barrel several times.

[0029] (4) Rinse the interfering copper ions with 1mL pure water;

[0030] (5) Pass 1mL of 25% methanol aqueous solution through the small column, and collect the eluate, which mainly contains gelatin solution;

[0...

Embodiment 2

[0036] The analysis object used in this example is the strong acid copper electrolyte containing gelatin in the electrolytic copper foil production workshop. Because gelatin is in strong acidic conditions, conventional GPC analysis is faced with strong acidity, the chromatographic column is easily damaged, and analysis is difficult. After pretreatment by solid phase extraction, the acidity is greatly weakened and the conditions are mild, which is suitable for subsequent GPC analysis.

[0037] 1. The solid phase extraction process is:

[0038] (1) Place the solid-phase extraction cartridge on the solid-phase extraction apparatus, and connect the top of the solid-phase extraction cartridge to a 50mL syringe through an adapter. For example, the HLB solid-phase extraction column of Waters Company is used, and the specification is 3cc60mg.

[0039] (2) First use 1mL of methanol, then activate the extraction cartridge with 1mL of water;

[0040] (3) Inject 300 mL of on-site sample...

Embodiment 3

[0048] The analysis object used in this example is the strongly acidic copper electrolyte containing gelatin at another sampling point in the electrolytic copper foil production workshop.

[0049] (1) Place the solid-phase extraction cartridge on the solid-phase extraction apparatus, and connect the top of the solid-phase extraction cartridge to a 50mL syringe through an adapter. The HLB solid-phase extraction column of Waters Company was used, and the specification was 6cc200mg.

[0050] (2) First use 2mL of methanol, then activate the extraction cartridge with 2mL of water;

[0051] (3) Inject 500 mL of on-site samples containing gelatin copper electrolyte from the syringe barrel several times.

[0052] (4) Rinse the interfering copper ions with 2mL pure water;

[0053] (5) Pass 2 mL of 25% aqueous methanol through the small column, and collect the eluate.

[0054] (2) Gel permeation chromatography is adopted, and the required instrument is a high performance liquid chrom...

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Abstract

This invention discloses one copper electrolyte clear gel molecule distribution test method, which comprises the following steps: first processing fix phase extracting; then selecting proper stuff fix phase small columns and injecting electrolyte liquid for first clear to isolate gel and interference element for once again; then removing the gel for small columns and collection the liquid; then using gel transparent spectrum method to analyze gel molecule content; using NaH2PO4, Na2SO4 and dodecyl sodium sulfate as flow phase; the column analysis adopts protein molecule column; the tester adopts ultraviolet tester and difference tester with wavelength of 190 to 230nm and temperature for 30 to 50 degrees.

Description

technical field [0001] The invention relates to a method for testing molecular weight distribution, in particular to a method for measuring the molecular weight distribution of gelatin in a small amount in copper electrolyte. Background technique [0002] Collagen refers to a type of protein that exists in animal tissues and organs. During extraction and separation, three products of collagen, gelatin and collagen can be produced with different methods and conditions. It is generally believed that the production of electrolytic copper foil requires that Cu 2+ The morphology of cathodic electrodeposition is controlled except for the Cu 2+ , H 2 SO 4 In addition to the control of concentration, liquid temperature, liquid flow rate, and current density, the addition of gelatin causes significant changes in the crystallization of copper foil, such as tensile strength, elongation, hardness, rough surface peak and valley shape, and so on. In order to produce copper foil produc...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/08
Inventor 陈健郭伟姜建国肖凯军郑必胜王定勇
Owner SOUTH CHINA UNIV OF TECH