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Micro fluid control chemistry sensor array chip using gel encapsule as base and preparing method

A chemical sensing and array chip technology, applied in the field of chemical sensing array chips, can solve the problems of low gel concentration requirements, difficult processing technology, unfavorable promotion and use, etc., to achieve wide application, reduce processing difficulty, and good sensing The effect of the characteristic

Inactive Publication Date: 2007-10-10
CHONGQING UNIV
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Problems solved by technology

However, this chip is only used for the detection of biofluorescence in cells, and does not perform more general chemical sensing detection, and has lower requirements in terms of gel concentration and other aspects.
Moreover, although the existence of micro-vias in the chip reduces the influence of sample diffusion, the alignment of micron-sized through-holes and micro-channels on the upper and lower layers is very difficult in the processing technology, which is not conducive to popularization and use.

Method used

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  • Micro fluid control chemistry sensor array chip using gel encapsule as base and preparing method
  • Micro fluid control chemistry sensor array chip using gel encapsule as base and preparing method
  • Micro fluid control chemistry sensor array chip using gel encapsule as base and preparing method

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Effect test

Embodiment 1

[0023] Referring to Figure 1 and Figure 2, the microfluidic chip is composed of three layers, the first layer is the carrier layer 1, made of glass, the second layer is the sensing array layer 2, and the third layer is the microfluidic channel layer 3, both layers are Made of PDMS. Layers II and III 2 and 3 have orthogonal microchannel networks 4, one for gel solidification and the other for sample flow. The microchannel network of layer II consists of ten parallel microfluidic channels A-J, and all channels in the microchannel network are filled with gel before combining with other layers. And the microchannel network of the third layer is composed of six parallel microfluidic channels K-P, which vertically intersect with the microchannels of the second layer 2 after the chip is combined. The two intersecting microchannel networks have a total of 60 intersections, and only at these specific intersections can the embedded gel be in contact with the sample solution flowing in ...

Embodiment 2

[0024] Embodiment 2: Utilize agarose gel to coat the calcium ion concentration of Fluo-3 sensing substance detection solution

[0025] Fig. 3 is a schematic diagram of the structure of the detection device using this microfluidic chip. A rubber tube with an inner diameter of 1 mm is used to connect the inlet 5 and outlet 6 of the chip and fix it with a bracket. The sample solutions contained in the six reagent bottles 7 flow through different channels in the flow channel network driven by the micro-flow peristaltic pump 8 . At each intersection point, the calcium ions in the solution diffuse into the gel below, and react with the Fluo-3 molecules therein, and emit fluorescence under the irradiation of laser light. A laser confocal microscope 9 is used to detect the fluorescence intensity of each intersection point.

[0026] When the 1 μM calcium chloride solution flows through the microfluidic channel K, the fluorescence intensity at the intersection point JK (marked as the i...

Embodiment 3

[0028] Embodiment 3: The preparation method of the chemical sensing array layer, see Fig. 6:

[0029] Firstly, the positive relief master of the target channel structure is processed on the silicon wafer by photolithography-wet etching technology. Once the master is processed, it can be reused many times. The pattern in the master plate exactly matches the dimensions of the channels in the elastic rubber.

[0030] When the master plate is finished, the required channel network is machined on the elastic rubber by replica molding. Before the molding operation, the silicon mother board was washed with water and methanol in sequence, and then blown dry with nitrogen.

[0031] Degassed elastomeric rubber, here PDMS, polymer prepolymer (Sylgard 184 silicone rubber, Dow Corning, Midland, MI) and hardener were formulated in a 10:1 weight ratio and poured onto the silicon master. After vacuum removing air bubbles, the PDMS is solidified on the motherboard after hardening for at leas...

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Abstract

A method for preparing sensing array chip of micro-fluid control chemistry based on gel encapsulation includes forming said chip by three layers as the first layer of load-carrying and the second layer of sensing array as well as the third layer of micro-fluid channel, distributing micro-fluid channel network on sensing array layer, packing gel encapsulated with chemical sensing material fuel of all micro-fluid channel, arranging micro-fluid channel network also on said micro-fluid channel layer and making sensing array layer be orthogonal with micro-fluid channel network on micro-fluid channel layer.

Description

technical field [0001] The invention belongs to the technical fields of bioengineering and analytical chemistry, and in particular relates to a chemical sensing array chip. Background technique [0002] In recent years, the research on integrating chemical and biological sensing devices into microfluidic chips as a detection method has been greatly developed. These microfluidic sensing devices are widely used in genetic analysis, drug screening, food quarantine and clinical analysis. It has important application value. Since highly integrated and parallel processing technology is conducive to the miniaturization of microfluidic chip devices, the parallel work of a large number of similar functional units can be used to improve analysis efficiency and achieve high-throughput analysis and screening. Therefore, sensing in microfluidic chips Array technology has been paid more and more attention. The rapid development of micro-processing technolog...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N35/00G01N31/00
Inventor 杨军刘向绍张丽果翟盛杰郑小林
Owner CHONGQING UNIV
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