Preparation and application of sage polysaccharides and its esters

A technology of sage and polysaccharide, which can be used in medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, plant raw materials, etc., and can solve the problem of no anti-HIV-1 activity.

Inactive Publication Date: 2007-12-12
陈朝银
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  • Abstract
  • Description
  • Claims
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But there are no research reports and patent applications about sage polysaccharides and their anti-HIV-1 activity

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  • Preparation and application of sage polysaccharides and its esters

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Embodiment 1

[0009] Embodiment 1: A kind of preparation method of compound sage polysaccharide in the present invention

[0010] Salvia yunnanensis C.H.Wright is taken, extracted with hot water, concentrated, ethanol precipitated, deproteinized, and freeze-dried to obtain the salvia polysaccharide of the present invention. The molecular weight measured by molecular sieve chromatography is 26,000 Daltons. The HPLC analysis shows that the monosaccharide composition is sorbitol, galactose, and rhamnose, and the ratio of the three is 5:2:2. The Smith degradation analysis shows that the rat tail There are no 1→2 glycosidic bonds in grass polysaccharides. Further according to the law of periodic acid consumption in polysaccharides, it is deduced that they are connected by 1→6 (or non-reducing ends), 1→4 and 1→3 glycosidic bonds, and the ratio of the three is 1: 3:1. Infrared spectrum analysis showed that at 905cm -1 and 847cm -1 There are absorption peaks on the left and right, which are the ...

Embodiment 2

[0011] Embodiment two: a kind of preparation method of sage polysaccharide sulfate ester in the present invention

[0012] Take 80mL of sulfonating agent and put it in a 250mL three-neck flask, control the temperature at 75°C, add 8 grams of clary sage polysaccharide, stir and react for 3h, take out the flask after the reaction and cool to room temperature, neutralize with 6mol NaOH solution, ethanol precipitation, centrifugation, The precipitate is dissolved in distilled water and dialyzed in distilled water, the dialyzed inner liquid is distilled and concentrated under reduced pressure, precipitated with ethanol, centrifuged, and dried to obtain the sage polysaccharide sulfate of the present invention. The molecular weight measured by molecular sieve chromatography is 18,000 Daltons, and the HPLC analysis shows that the monosaccharide composition is sorbitol, galactose, and rhamnose, and the ratio of the three is 5:2:2, and the degree of sulfation is 1.7. According to the re...

Embodiment 3

[0013] The pharmacological action of clary sage polysaccharide and sulfate ester thereof in embodiment three present invention

[0014] 1. The cytotoxicity of sage polysaccharide sulfate was determined by MTT method, and the anti-HIV-1Ba-L (R5 virus) activity of sage polysaccharide sulfate was determined by fluorescence measurement technology.

[0015] TZM-b1 cell is a HeLa cell line expressing HIV-1 receptors and co-receptors such as CD4, CXCR4 and CCR5, as well as firefly enzyme expressed under the control of HIV-1 promoter (AIDS Research and References Reagent Program, NIAID, NIH). First, the toxicity of sage polysaccharide sulfate to TZM-b1 cells was determined. The method is as follows: on a 96-well flat-bottomed culture plate, the sage polysaccharide sulfate is diluted serially by 2 times with RPMI-1640 medium. A total of 6 dilutions, 100 μl per well, set up 3 replicate wells, set up a cell control group and a blank control group. Then, add 100 μl containing 3×10 to e...

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Abstract

The invention relates to the preparation and application of Salvia polysaccharide and its esters. The sugar chain is sorbitol, rhamnose, and galactose linked through 1->6 (or non-reducing terminal), 1->4 and 1->3 glycosidic bond. The invention provided Salvia polysaccharide and its esters is prepared from Salvia as raw material or residues of Salvia after extracting fat-soluble effective ingredient or (and) water soluble phenolic acid by leaching with hot water, concentrating, precipitating with ethanol, deproteinizing to obtain Salvia polysaccharide, and esterizing to obtain Salvia polysaccharide ester (sulfate, phosphate ester, nitric ether and acetic ester).The invention obtained Salvia polysaccharide and its esters both have strong human immunodeficiency virus and herpes simplex virus resisting activity, has low toxicity to human cell, and can be used for preparing preparation for preventing or treating AIDS viral and herpes simplex viral infection.

Description

technical field [0001] The present invention relates to the preparation and application of clary sage polysaccharide and its esters. The clary sage polysaccharide and its esters all have high anti-human HIV and human herpes simplex virus activity, and high selectivity index, and are good for preparing preparations for preventing or treating human HIV infection and herpes simplex virus infection. application prospects. Background technique [0002] According to "Chinese Drugs", there are 83 species of Salvia plants in China, 25 varieties, and 9 variants, of which there are more than 30 species (including variants and variants) for medicinal purposes, and most of them use their roots as medicines for Danshen. Such as Salvia miltiorrhiza (Salvia miltiorrhiza, Salvia miltiorrhiza), S. bowleyana, S. przewalskii, S. yunnanensis (Salvia miltiorrhiza), Shamrock S. trijuga, S. plectranthoides and S. digitaloides (Teng Yanfen, et al. China Wild Plant Resources, 2001, 2: 1-3), And s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P31/18A61P31/22C08B37/00A61K125/00
Inventor 赵声兰陈朝银李立
Owner 陈朝银
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