Preparation method for separating and purifying water-soluble protein of ginseng

A water-soluble protein, separation and purification technology, applied in the field of separation, purification and preparation of ginseng water-soluble protein, can solve the problems of no antifungal, antiviral and anti-proliferation activities, and achieve a short cycle, quick effect and simple purification process. Effect
CN101092448AInactive Publication Date: 2007-12-26CHANGCHUN UNIV OF CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHANGCHUN UNIV OF CHINESE MEDICINE
Publication Date
2007-12-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention relates to a method for preparing ginseng proteins by buffer extraction, ammonium sulfate precipitation and affinity chromatography, ion exchange chromatography, hydrophobic chromatography, gel filtration chromatography. The method comprises: extracting ginseng juice, adding solid ammonium sulfate to 80% saturation, performing equilibrium dialysis with Tris-HCl buffer, centrifuging at a high speed, performing ultrafiltration, and purifying by different kinds of chromatography to obtain 5 kinds of inseng proteins GP1-GP5. GP1 is RNA-like enzyme. GP3 is chitinase-like protein. GP4 is ginsengribonuclease. GP2 and GP5 are reported for the first time. The method has such advantages as good purification effect, large loading volume and high sample yield, and can be scaled up for mass production. The samples, except for GP4, show a single band in SDS-PAGE polyacrylamide gel electrophoresis, which confirms that the samples reach electrophoretic pure.
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Description

technical field

[0001] The invention belongs to the technical field of protein separation and purification, and in particular relates to a preparation method for separation and purification of ginseng water-soluble protein by combining buffer extraction, ammonium sulfate precipitation and various column chromatography techniques. Background technique

[0002] The research on ginseng protein and peptides began in the 1960s, mainly focusing on the extraction method of ginseng peptides. In 1963, F.Gstirnor et al. of Germany detected a variety of amino acids from ginseng root for the first time: glutamic acid, cysteine, tyrosine, etc. In 1966, they (Arch Pharm.1966, 299(11): 934-937) used electrophoresis to obtain 5 small peptides from the methanol water extract of Korean white ginseng, but the primary structure was not determined. In 1980, Japanese Ando and Okada (Planta Medica. 1980, 38: 18-21) found that there is a substance in ginseng water extract that strongly inhibits ad...

Claims

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