Technique for preparing phosphatidyl serine rich in highly-unsaturated fatty acid

An unsaturated fatty acid, phosphatidylserine technology, applied in the direction of fermentation, etc., can solve the problems of complicated product separation steps, low product yield, long reaction time, etc., and achieves a product that is conducive to reuse, high product yield, and fast reaction speed. Effect

Inactive Publication Date: 2008-04-09
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme-catalyzed reactions in the past were mainly carried out in traditional organic solvents (n-hexane, etc.), the reaction time was long, the product separation steps were cumbersome, and the product yield was low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Heat the reactor to 40°C, weigh 75 mg of squid lecithin, and place it in the reactor; weigh 0.1 g of anhydrous calcium chloride (for maintaining the stability of the enzyme), and 0.85 g of solid L-serine, dissolve in 2.4 Add phosphate buffer solution of mlpH5.5 into the reaction kettle; accurately weigh 9.6U of phospholipase D and add it into the reaction kettle; ) storage tank, the R134a gas is liquefied by the condenser and then pressurized by the plunger pump into the reaction kettle. After the reactor pressure reached 6MPa, R134a reached the subcritical state. Stop the plunger pump, close the storage tank, and stir the reaction. After reacting for 3.5 hours, the shut-off valve was opened to reduce the pressure, and the reaction mixture was allowed to enter a separation tank with a temperature of 40° C. connected to the reaction tank. Turn on the compressor to recover R134a from the separation tank; after the recovery is complete, collect the reaction mixture from ...

Embodiment 2

[0009] Heat the reaction kettle and the separation kettle to 40°C, weigh 300mg of squid lecithin, and place it in the reaction kettle; weigh 0.4g of anhydrous calcium chloride, 3.5g of solid L-serine, and dissolve them in 10ml of phosphate buffered saline with a pH of 5.5 Add liquid into the reaction kettle; accurately weigh phospholipase D38U, and add it into the reaction kettle; after the system is sealed, open the R134a storage tank, let the R134a gas liquefy through the condenser, and then pressurized by the plunger pump into the reaction kettle. After the reactor pressure reached 6MPa, the plunger pump was stopped, the storage tank was closed, and the reaction was stirred. After reacting for 3.5 hours, the shut-off valve was opened to reduce the pressure, and the reaction mixture was allowed to enter a separation tank with a temperature of 40° C. connected to the reaction tank. Turn on the compressor to recover R134a from the separation tank; after the recovery is complet...

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Abstract

The invention relates to a technique for preparing diacylglyceryl-phosphorylserine rich in unsaturated acid, which is characterized in that a reactor is preheated to 30 to 60 DEG C, squid lecithin and L-serine at the mass ratio of 1:1-15 is added, phosphatidase D2-15U / g is also added as reaction substrate, gas R134a is pumped to lead the pressure of the reactor to reach 4-6MPa, the pressure is reduced after agitating reaction for 2 to 8 hours, R134a is recycled after the reaction mixture comes into a separation pot which is communicated with the reactor under the temperature of 30 to 60 DEG C, reaction mixture is collected from the a sample receiving port of the separation pot, at last, the reaction mixture is washed by using acetone and centrifuged for 5 to 10 min to separate the solid phase for further purification. The diacylglyceryl-phosphorylserine prepared by the invention rich in high unsaturated fatty acid as the medium in catalytic reaction of phosphatidase D has the advantages of simple technique, low equipment input, short reaction time, high content of unsaturated fatty acid and high yield and the neglecting of organic solvent. Furthermore, R134a can be completely recycled after reaction, which is beneficial to the separation of products and repeated use of enzyme.

Description

technical field [0001] The invention relates to a process for preparing phosphatidylserine rich in polyunsaturated fatty acids. Background technique [0002] The n-3 polyunsaturated fatty acids (n-3 PUFA) in fish oil, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have the functions of preventing and treating cardiovascular diseases, anti-inflammatory Physiological activities such as anti-cancer, enhancing immunity, helping brain development and protecting eyesight. Phosphatidylserine (PS) is an important component of mammalian cell membranes, and is widely used as liposomes and emulsifiers in medicine, cosmetics, and food industries. Phosphatidylserine also has the activity of improving memory function in patients with senile memory impairment and Alzheimer's disease. The process of extracting phosphatidylserine from natural products is complicated, the yield is low, and the product is expensive, which limits the further extensive application of ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00
Inventor 李兆杰于刚薛长湖薛勇王玉明唐庆娟
Owner OCEAN UNIV OF CHINA
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