Method for preparing n-3PUFA ocean glycerin ester by enzymatical process

An enzymatic preparation and glyceride technology, applied in the fields of biotechnology and functional health care, can solve problems such as being in the blank, and achieve the effects of improving utilization rate, preventing oxidation, and easily controlling the reaction process

Inactive Publication Date: 2008-04-16
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the preparation of n-3PUFA marine glycerides in my country mainly uses the selective hydrolysis or transesterification of lipase, but the research on the preparation of n-3PUFA marine glycerides by lipase-catalyzed synthesis method is still blank

Method used

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Examples

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Effect test

example 1

[0020] Add n-hexane 7mL, glycerol 5g, n-3 PUFA concentrate 1g (from tuna fish oil, DHA and EPA content are 72% and 17%, respectively), 0.6% (w / w) water, 40mg lipase in the reaction vessel Novozym435, sealed under nitrogen protection, carried out esterification reaction at 150 times / min in a constant temperature water-bath shaker at 40°C, and after the reaction was carried out for 1 hour, 1 g of molecular sieve was added to remove the moisture generated by the reaction. After the reaction is finished, filter the reaction mixture synthesized by lipase catalysis through an anhydrous sodium sulfate bed to remove enzyme molecules and molecular sieves, and titrate the filtrate with 0.025mol / L sodium hydroxide solution (phenolphthalein as indicator) until the solution is reddish , Transfer the reaction solution to a separatory funnel, add 25 mL of n-hexane, mix well, discard the lower aqueous solution, collect the upper n-hexane layer (containing acylglycerol), and dehydrate with anhy...

example 2

[0022] Add n-hexane 5mL, glycerin 3g, n-3 PUFA concentrate 0.5g (from skipjack fish oil, DHA and EPA content are 68% and 15% respectively), 0.5% (w / w) water, 20mg in the reaction vessel Lipase Novozym435, sealed under nitrogen protection, carried out esterification reaction at 150 times / min in a constant temperature water-bath shaker at 40°C. After the reaction was carried out for 1 hour, 1 g of molecular sieve was added to remove the water generated by the reaction. After the reaction is finished, filter the reaction mixture synthesized by lipase catalysis through an anhydrous sodium sulfate bed to remove enzyme molecules and molecular sieves, and titrate the filtrate with 0.025mol / L sodium hydroxide solution (phenolphthalein as indicator) until the solution is reddish , Transfer the reaction solution to a separatory funnel, add 25 mL of n-hexane, mix well, discard the lower aqueous solution, collect the upper n-hexane layer (containing acylglycerol), and dehydrate with anhydr...

example 3

[0024] Add 10 mL of n-hexane, 7 g of glycerol, 1 g of n-3 PUFA concentrate (from snake mullet fish oil, with DHA and EPA content of 48% and 20%, respectively), 0.8% (w / w) water, and 80 mg of fat in the reaction vessel The enzyme Novozym435 was sealed under nitrogen protection, and the esterification reaction was carried out in a constant temperature water bath shaker at 40°C at 150 times / min. After the reaction was carried out for 1 hour, 1 g of molecular sieve was added to remove the water generated by the reaction. After the reaction is finished, filter the reaction mixture synthesized by lipase catalysis through an anhydrous sodium sulfate bed to remove enzyme molecules and molecular sieves, and titrate the filtrate with 0.025mol / L sodium hydroxide solution (phenolphthalein as indicator) until the solution is reddish , Transfer the reaction solution to a separatory funnel, add 25 mL of n-hexane, mix well, discard the lower aqueous solution, collect the upper n-hexane layer (...

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Abstract

A method for preparing n-3PUFA marine glyceride through adopting enzyme method includes following steps: refined fish oil is made into n-3PUFA condensate through saponification, acidification and urea adduct method; lipase catalyzes the synthesis reaction between n-3PUFA and glycerol; n-3PUFA marine glyceride is extracted and separated; moreover, n-3PUFA is effectively concentrated in glyceride to be made into functional products which can prevent and cure cardiovascular diseases and nourish the brain and improve intelligence. The raw material of the invention, n-3PUFA, is extracted from natural fish oil, thereby being safe and effective; during production, nitrogen protection is adopted without adding synthetic antioxidant, thereby effectively preventing oxidation of n-3PUFA; with scientific and reasonable technique, the invention completes synthesis by means of biological enzyme method along with high esterification degree of reaction, higher utilization rate of raw material and easily controlled reaction process; therefore, the invention has wide market prospect.

Description

technical field [0001] The invention relates to a method for catalyzing and synthesizing n-3PUFA marine glyceride by using lipase, which belongs to the field of biotechnology and functional health care. Background technique [0002] In the present invention, "n-3PUFA" refers to n-3 polyunsaturated fatty acid; "DHA" refers to docosahexaenoic acid, and "EPA" refers to eicosapentaenoic acid. DHA and EPA in natural fish oil mainly exist in the form of triglycerides, their relative content is relatively low, and the health care and medical effects are relatively poor, so it is difficult to meet the needs of consumers. In order to improve the health care and medical effects of DHA and EPA, many researchers have transesterified or hydrolyzed DHA and EPA in glycerides into corresponding ethyl esters or free forms, and then increased DHA and EPA by various physical or chemical methods. content, in order to enhance the health care and medical effects of DHA and EPA. In 1990, after a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 刘书成章超桦洪鹏志吉宏武杨萍郝记明张静曹文红
Owner GUANGDONG OCEAN UNIVERSITY
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