Method for producing lacitc acid by non-calcium autocycle continuous fermentation salt fermentation

A technology of automatic circulation and calcium salt method, applied in the biological field, can solve the problems of long process flow, low yield, low conversion rate, etc., and achieve the effects of prolonging metabolism time, improving fermentation efficiency and improving conversion rate

Inactive Publication Date: 2008-07-23
ZHAODONG AIER LACTIC ACID
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] This process is used in the fermentation process and the batch single-tank fermentation after the strain is transferred and multiplied has low fermentation automation control, easy to infect bacteria, degradation, and variation, resulting in large fluctuations in the quality of the fermentation broth, low conversion rate, and low utilization of equipment, resulting in fermentation equipment. Large one-time investment
And because of the use of CaCO in the fermen

Method used

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  • Method for producing lacitc acid by non-calcium autocycle continuous fermentation salt fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In this embodiment, the non-calcium salt method automatic cycle continuous fermentation process for producing lactic acid comprises the following steps:

[0039] 1. Slurry preparation: first adjust 950kg cornstarch to a concentration of 30% by weight at a temperature of 50°C, pH 6.0, and the amount of amylase to be 12u / g.

[0040] 2. Liquefaction: The temperature is controlled at 98°C, the time is 30 minutes, and the DE value is controlled at 18.

[0041] 3. Saccharification: the temperature is controlled at 60°C, the pH value is 4.5, the time is 30 hours, the DE value is 95, and the amount of glucoamylase used is 80u / g.

[0042] 4. Ingredients: The five kinds of ABCDE medium formulas used in the present invention are in different fermentation stages, and the temperature of the medium is all controlled at 60° C., and the pH value is 6.

[0043] Medium formula A is: glucose 150kg / m 3 , ammonium lactate ≤ 1.5%, WPH 5kg / m 3 , NH 4 h 2 PO 4 0.5kg / m 3 , KH 2 PO 4 ...

Embodiment 2

[0056] The basic process is the same as in Example 1, except that the pulping process is as follows: the starch concentration is controlled at 25%, the temperature is 60°C, the pH is 6.5, and the amount of amylase is 8u / g.

[0057] The liquefaction process is as follows: the temperature is controlled at 100°C, the time is 40 minutes, and the DE value is controlled at 15.

[0058] The saccharification process is as follows: the temperature is controlled at 62°C, the pH value is 4.8, the time is 40 hours, the DE value is 98, and the amount of saccharification enzyme used is 150u / g.

[0059] Medium formula A is: glucose 120kg / m 3 , sodium lactate ≤ 1.5%, WPH 8kg / m 3 , NH 4 h 2 PO 4 1.5kg / m 3 , KH 2 PO 4 0.9kg / m 3 , MgSO 4 ·7H 2 O 0.5kg / m 3 , MnSO 4 ·H 2 O 0.05kg / m 3 . Prepare with sterile deionized water.

[0060] The difference between medium B and medium A is: WPH 10kg / m 3 , KH 2 PO 4 0.05kg / m 3 .

[0061] The composition of medium C is basically the sam...

Embodiment 3

[0066] The basic process is the same as in Example 1, except that the pulping process is as follows: the starch concentration is controlled at 28%, the temperature is 55°C, the pH is 6.2, and the amount of amylase is 15u / g.

[0067] The liquefaction process is as follows: the temperature is controlled at 105°C, the time is 35 minutes, and the DE value is controlled at 22.

[0068] The saccharification process is as follows: the temperature is controlled at 60°C, the pH value is 4.6, the time is 35 hours, the DE value is 96, and the amount of glucoamylase used is 120u / g.

[0069] Medium formula A is: glucose 60kg / m 3 , potassium lactate ≤1.5%, WPH 10kg / m 3 , NH 4 h 2 PO 4 1.0kg / m 3 , KH 2 PO 4 1.0kg / m 3 , MgSO 4 ·7H 2 O 0.6kg / m 3 , MnSO 4 ·H 2 O 0.01kg / m 3 . Prepare with sterile deionized water.

[0070] The difference between medium B and medium A is: WPH 15kg / m 3 , KH 2 PO 4 0.01kg / m 3 .

[0071] The composition of medium C is basically the same as tha...

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Abstract

The invention provides a non-calcium-salt lactic acid production method using automatic circulating continuous fermentation, which is different from the calcium salt method and comprises steps that: size mixing, liquefaction, saccharification, burden, continuous sterilization, membrane filtration, separation of lactic acid, purification and evaporation; the system process water can be treated and recycled respectively through the steps of continuous fermentation and ultra filtration, electrodialytic coupled purification and separation as well as secondary reverse osmosis. So in the prior traditional calcium salt method, the strain transfer and proliferative fermentation problems as using intermittent single tanks are solved; strain automatic circulating is realized as using the continuous fermentation equipment and technique. The non-calcium-salt lactic acid production method has the advantages of improved equipment capacity factor and fermentation conversion rate, prolonged strain metabolism time, improved conversion rate of the carbon source and the specific production rate of the fermentation process, zero-emission of the environmental pollution in the fermentation process and significantly improved product quality; thereby the non-calcium-salt lactic acid is suitable for commercial production of organic acid fermentation.

Description

technical field [0001] The invention relates to a method for producing lactic acid by automatic circulation and continuous fermentation by a non-calcium salt method, belonging to the field of biotechnology. Background technique [0002] The traditional fermentation process adopts the organic acid calcium salt method, which has problems such as long production process, low degree of automation, serious pollution, high cost, and poor product quality. [0003] The process route of the traditional calcium salt method fermentation to produce organic acids is: corn, starch pulping → liquefaction → fermentation → plate and frame filtration → purification treatment → evaporation → acid hydrolysis reaction → decolorization → mash separation → decolorization → fine filtration → concentration → Ion exchange→activated carbon column decolorization→membrane filtration→concentration→finished product packaging. [0004] This process is used in the fermentation process and the batch single-...

Claims

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Application Information

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IPC IPC(8): C12P7/56
Inventor 任晓峰
Owner ZHAODONG AIER LACTIC ACID
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