Method for preparing immobilized enzyme biological catalyst

Abstract

The invention designs a process for preparation, which utilizes silica-based mesoporous molecular sieves and calcium alginate to fix enzyme biocatalyst, and belongs to the technical field of fixing enzyme biocatalyst preparation technique. The process for preparation comprises utilizing silica-based mesoporous molecular sieves MCM-41 to be carrying agent, fixing biocatalyst molecule in meso-porous pore path of silicon oxide carrier, secondarily fixing immobilized enzyme biocatalyst which is prepared in calcium alginate gel. The process for preparation of the invention adopts a physical adsorption embedding method and a collosol-gel method. The fix enzyme biocatalyst of the invention has better enzyme dispersibility and higher enzymes loading, and has higher enzymatic activity recovery ratio and repeated utilization ratio. The invention can fix various enzymes such as horse radish peroxidase, proteinase, lipase and the like. The fix enzyme biocatalyst of the invention can be used to process waste water which contains hydroxybenzene and decolorize, which has higher catalytic activity.

Description

technical field [0001] The invention designs a preparation method using silicon-based mesoporous molecular sieve and calcium alginate immobilized enzyme biocatalyst, which belongs to the technical field of immobilized enzyme biocatalyst preparation technology. Background technique [0002] The synthesis of ordered mesoporous molecular sieves began as early as the 1970s. Only the report of Mobil's MCM-41 and other mesoporous molecular sieves in 1992 attracted widespread attention, and it was considered to be the first step in the synthesis of ordered mesoporous molecular sieves. the real start. They used surfactants as templates to synthesize M41S series mesoporous molecular sieves. The synthesis of ordered mesoporous molecular sieve series M41S with large specific surface area, regularly arranged and adjustable channels is known as a milestone in the history of molecular sieve development. After that, various synthetic systems and synthetic routes came out. These ordered m...

AI Technical Summary

Problems solved by technology

However, the activity of the enzyme is affected by the preparation conditions of the sol-gel, and the narrow grid pores limit the mass transfer; at the same time, the sol-gel method is difficult to control the distribution of the pore size, and the selectivity to the particle size of the immobilized substance is very small. ; and the enzyme can still coagulate in this kind of matrix, and the carrier with fixed pore distribution will help prevent the coagulation of the enzyme

In addition, water-soluble enzymes are prone to inactivation, poor stability, and can only be used once, resulting in high processing costs

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