Method for immobilizing lipase by block polymer micelle

Abstract

The invention provides a method for immobilizing lipase by block polymer micelle. The method comprises the following steps: dithiodipropionic acid is activated by an activator to activate a carboxyl group and reacted with hyaluronic acid to prepare an intermediate, the intermediate is activated by the activator to react with polylactic acid-glycolic acid to obtain a block polymer, and the block polymer is dissolved in deionized water to prepare a block polymer micelle solution; the lipase powder is dissolved in a phosphate buffered saline solution of pH being 8.0, and the material is stirred and centrifuged, a supernatant is transferred to a volumetric flask, and a volume is metered with a buffer solution of pH being 8.0 to obtain enzymatically synthesized cyhalofoprin lipase; the polymermicelle solution is mixed with the enzymatically synthesized cyhalofoprin lipase, a mixture is filtered, deionized water is added for dissolving, and washed with the buffer solution of pH being 8.0 toremove excess lipase, a volume is metered to the 1L of a phosphate buffer solution of the pH being 8.0, and lipase immobilized by block polymer micelle can be obtained after freeze drying. The process of the invention is simple.

Description

technical field [0001] The invention belongs to the field of polymers, and more specifically relates to a method for immobilizing lipase in block polymer micelles. Background technique [0002] Lipase is present in basically all living organisms, and it plays a key role in the digestion, transport and shearing of lipids (eg, triglycerides, fats, oils, etc.). Lipase can catalyze various reactions such as hydrolysis reaction, alcoholysis reaction, transesterification reaction and hydrolysis reverse synthesis of esters. It has the characteristics of high stability, wide substrate specificity, high catalytic activity and few side reactions. an important biocatalyst. Therefore, lipase has important applications in the energy field, food industry, detergent, oil industry, pharmaceutical industry, agriculture and many other fields. [0003] As a green biocatalyst, lipase has a series of advantages such as high catalytic activity, less side reactions, low pollution, and high opera...

AI Technical Summary

Problems solved by technology

[0003] As a green biocatalyst, lipase has a series of advantages such as high catalytic activity, less side reactions, low pollution, and high operational stability. However, lipase itself also has some limitations in practical industrial applications: Insufficient stability under conditions such as solvents, strong acids or strong bases, volatile inactivation, poor tolerance to the environment; easy to be degraded, prone to agglomeration during the reaction process, and difficult to fully contact with the substrate

In short, the synthesis of cyhalofop-ethyl by chemical synthesis method has poor selectivity, low total yield, and high environmental treatment cost, which is not in line with the current development trend of green chemical industry

[0006] Chinese patent CN103255125A discloses a method for preparing block polymer-immobilized enzymes. In this synthesis method, polymethyl methacrylate-ethyl acrylate nanofiber membranes are prepared. The extraction process is complicated and the cost is high, so it is difficult to be widely used in industrial production.

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