Method for manufacturing conductive polyaniline/cellulose composite biosensor

A conductive polyaniline and biosensor technology, which is applied in instruments, scientific instruments, and material analysis through electromagnetic means, can solve the problems of high cost, limit the wide application of sensors, limit the wide application of biosensors, etc., and achieve low production cost, Renewable and low cost, easy to industrialize the effect of production

Inactive Publication Date: 2008-08-27
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The absence of conventional equipment to fabricate stable and reproducible conductive polymer biosensors has largely limited the wide application of such sensors in the field of analysis.
Although bioactive substances can be immobilized in conductive polymers by electrochemical polymerization, these methods require noble metals as electrodes, which are expensive, which also limits the wide application of biosensors

Method used

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  • Method for manufacturing conductive polyaniline/cellulose composite biosensor
  • Method for manufacturing conductive polyaniline/cellulose composite biosensor
  • Method for manufacturing conductive polyaniline/cellulose composite biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026](1) Preparation of cellulose: After crushing corn stalks into 20-100 mesh powders, dissolve them in a nitric acid solution with 15 times the weight of corn stalks and a concentration of 8%, distill at 110°C for 2 hours under magnetic stirring, and then Add 7 times the weight of corn stalks and 3% sodium hydroxide solution, continue distillation for 1.5 hours, cool the reaction mixture to room temperature, wash with distilled water until it is colorless, and then wash with ethanol several times to remove residual Oligosaccharides. Finally, it was dried in a drying oven at 40° C. for 5 hours. The cellulose is light yellow and appears as a ribbon or flat rod under the microscope. This structure is conducive to the attachment of aniline monomers.

[0027] (2) Preparation of conductive polyaniline / cellulose conductive composite material: first dissolve aniline monomer in hydrochloric acid solution at room temperature, and continue to stir until aniline hydrochloride is compl...

Embodiment 2

[0031] (1) Preparation of cellulose: after crushing wheat straw into 20-100 mesh powder, dissolve it in a nitric acid solution with 20 times the weight of the straw and a concentration of 6%, distill at 100°C for 2.5 hours under magnetic stirring, and then add 10 times the weight of corn stalks and a concentration of 5% sodium hydroxide solution, after continuing to distill for 1 hour, the reaction mixture was cooled to room temperature, washed with distilled water until it was colorless, and then washed with ethanol several times to remove residual low polysaccharides. Finally, it was dried in a drying oven at 40° C. for 5 hours. The cellulose is light yellow and appears as a ribbon or flat rod under the microscope. This structure is conducive to the attachment of aniline monomers.

[0032] (2) Preparation of conductive polyaniline / cellulose conductive composite material: Dissolve aniline monomer in hydrochloric acid solution at room temperature, and keep stirring until anil...

Embodiment 3

[0036] (1) Preparation of cellulose: After crushing corn stalks into 20-100 mesh powders, dissolve them in a nitric acid solution with 18 times the weight of corn stalks and a concentration of 5%, distill at 120°C for 2.5 hours with magnetic stirring, and then Add 10 times the mass of corn stalks and a 4% sodium hydroxide solution, continue distillation for 1.5 h, then cool the reaction mixture to room temperature, wash with distilled water until it is colorless, and then wash with ethanol several times to remove residual Oligosaccharides. Finally, it was dried in a drying oven at 40 °C for 5 h. The cellulose is pale yellow and appears as a band or flat rod under the microscope.

[0037] (2) Preparation of conductive polyaniline / cellulose conductive composite material: Dissolve aniline monomer in hydrochloric acid solution at room temperature, and keep stirring until aniline hydrochloride is completely dissolved; then dissolve cellulose with 2.5 times the mass of aniline mono...

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Abstract

The invention discloses a conductive polyaniline / cellulose composite material biosensor, wherein, firstly, a given amount of celluloses are added into aniline hydrochloride solution, and conductive aniline / cellulose composite materials are prepared after oxidative polymerization of anilines; secondly, conductive polyaniline / celluloses are then placed into buffer containing bioactive substances for immersion of 4 to 5 hours, and the bioactive substances are fully attached to the surface of a polyaniline sheet; thirdly, a given amount of polyaniline / cellulose composite materials provided with the bioactive substances are mixed with a proper quantity of conductive inks to form mash; fourthly, a saturated Ag / AgCl electrode is taken as a reference electrode and a carbon electrode is taken as an auxiliary electrode, and then the three-electrode conductive polyaniline / cellulose composite material biosensor is formed by the reference electrode, the auxiliary electrode and an operation electrode which is coated by compounds. The conductive polyaniline / cellulose composite material biosensor provided by the invention has the advantages of low cost, simple composition method, no pollution, high production efficiency, and easy realization of industrialization.

Description

technical field [0001] The invention belongs to the field of biological materials and relates to a biosensor, in particular to a method for preparing a conductive polyaniline / cellulose composite biosensor prepared from natural cellulose and a conductive polymer. Background technique [0002] Due to their special structure and excellent physical and chemical properties, conductive polymers have broad application prospects in the fields of optics, electromagnetism and chemistry. In recent years, the application of conductive polymers in the field of biomaterials has achieved certain research results. [0003] The application of conductive polymers in the field of biomaterials is most common as biosensors. Usually, bioactive substances such as different enzymes, coenzymes, antibodies, DNA, and even cells and tissues are immobilized in conductive polymers to form various new biosensors. The sensor has the characteristics of strong response performance, simple and controllable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 莫尊理赵仲丽史华锋陈红
Owner NORTHWEST NORMAL UNIVERSITY
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