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Grease catalysis separation biphasic enzyme-film bioreactor and its preparation and application

A technology of membrane bioreactor and oil ester, applied in the direction of enzyme production/bioreactor, textile and paper making, fixed on/in organic carrier, etc., can solve enzyme shedding, difficult to catalyze macromolecular substrate reaction, Solve the problems of weak binding between enzyme protein and carrier, achieve high specific surface area and porosity, improve biological activity and stability, and improve enzyme utilization

Inactive Publication Date: 2011-01-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the physical embedding method for enzyme immobilization, the binding force between the enzyme protein and the carrier is weak, the enzyme is easily affected by external factors and falls off, the catalytic reaction is limited by the mass transfer resistance, and it is not easy to catalyze the reaction of the macromolecular substrate

Method used

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  • Grease catalysis separation biphasic enzyme-film bioreactor and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Dissolve amino-containing polyphosphazene with a molecular weight of 500,000 in chloroform to form a spinning solution with a concentration of 12wt.%. The spinning voltage is 5kV, the flow rate of the spinneret solution is 2.0mL / h, and the receiving distance is 10cm. Next, electrospinning is carried out to make a superfine fiber composite membrane, and polyester non-woven fabric is used as a supporting material, which is filled in an inner and outer double cylindrical membrane tool to form a membrane device. Connect the prepared polyphosphazene microfiber membrane devices in series to the device shown in Figure 1 to form a membrane reactor, first inject glutaraldehyde into the membrane reactor by means of cross-flow filtration, and react in the membrane at 20°C Circulate in the membrane reactor device for 3 hours, and then circulate the lipase solution with a concentration of 5g / L in the membrane reactor device for 3 hours at 20°C, so as to realize the activation and enz...

Embodiment 2

[0028] Dissolve amino-containing polyphosphazene with a molecular weight of 600,000 in chloroform to prepare a spinning solution with a concentration of 1wt.%. The spinning voltage is 22kV, the flow rate of the spinneret solution is 0.1mL / h, and the receiving distance is 10cm. Next, electrospinning is carried out to make a superfine fiber composite membrane, and polyester non-woven fabric is used as a supporting material, which is filled in an inner and outer double cylindrical membrane tool to form a membrane device. Connect the prepared polyphosphazene microfiber membrane devices in series to the device shown in Figure 1 to form a membrane reactor, first inject glutaraldehyde into the membrane reactor by means of cross-flow filtration, and react in the membrane at 20°C Circulate in the membrane reactor device for 3 hours, and then circulate the lipase solution with a concentration of 5g / L in the membrane reactor device for 3 hours at 20°C, so as to realize the activation and ...

Embodiment 3

[0030] The amino-containing polyphosphazene with a molecular weight of 700,000 was dissolved in chloroform to form a spinning solution with a concentration of 9wt.%. The spinning voltage was 30kV, the flow rate of the spinneret solution was 0.5mL / h, and the receiving distance was 5cm. Next, electrospinning is carried out to make a superfine fiber composite membrane, and polyester non-woven fabric is used as a supporting material, which is filled in an inner and outer double cylindrical membrane tool to form a membrane device. Connect the prepared polyphosphazene microfiber membrane devices in series to the device shown in Figure 1 to form a membrane reactor. First inject glutaraldehyde into the membrane reactor by means of cross-flow filtration, and react in the membrane at 25°C. Circulate in the membrane reactor device for 2.5 hours, and then circulate the lipase solution with a concentration of 5g / L in the membrane reactor device for 2.5 hours at 25°C, so as to realize the ac...

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Abstract

The invention relates to a grease catalyzing and separating biphasic enzyme-membrane reactor and a method for preparing and using hydrolyzed edible glyceride. The method comprises the steps of filling polyphosphazene superfine fiber complex film into a mould with an inner and an outer cylinders to form a roll membrane device; sequentially injecting activating agent solution and lipase solution into the membrane device, and causing the lipase to be chemically fixed on the surface of the superfine fiber film to obtain an enzyme-membrane reactor; and injecting edible glyceride and phosphate buffer respectively into the inner side and the outer side of the superfine fiber film to conduct hydrolysis reaction on the surface of the superfine fiber surface. The reactor can be used for catalyzingthe hydrolysis of the glyceride, and for producing diglyceride or monoglyceride edible oil and extracting functional lipid by treating lard or fish oil. The glyceride hydrolyzate accounts for more than 85%. The grease catalyzing and separating biphasic enzyme-membrane reactor is designed by combining efficient biocatalysis and complex film separation, and has the advantages of repeatable use, realized continuous efficient production, reduced cost, saved resource and good development prospect.

Description

technical field [0001] The invention relates to a two-phase enzyme-membrane bioreactor for catalyzing the separation of oil and ester, its preparation and its application in catalyzing the hydrolysis of edible glycerolipids. Background technique [0002] Immobilized enzyme technology is one of the core technologies of enzyme engineering, which is of great significance to the food industry. Immobilized enzymes have the advantages of easy continuous production, easy product separation and purification, improved enzyme stability, easy control of reaction conditions, and improved product quality. my country's immobilized enzyme research began in 1970. First, the Institute of Microbiology of the Chinese Academy of Sciences and the Shanghai Institute of Biochemistry (now the Shanghai Institute of Biochemistry and Cells) started the study of immobilized enzymes at the same time. At present, researchers at home and abroad have carried out fruitful research and development on food e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/40D01F6/76C08J5/18C08L85/02D06M13/11D06M13/123C12N11/08C12P7/64C12P7/20D06M101/30
Inventor 黄小军徐志康万灵书黄赋余安国
Owner ZHEJIANG UNIV