Preparation of thiophilic magnetic compound microballoons and method for separating immune globulin G using the compound microballoons

Abstract

The invention provides a preparation method for a thiophilic composite microsphere and a novel method for separating and purifying serum immunoglobulin G (IgG) with high purity and high activity in batch, which has simple and convenient operation. In the invention, ferroferric oxide particles with superparamagnetism are prepared by redox reaction, magnetic microspheres are prepared by miniemulsion polymerization technology, the full and complete coating of magnetic particles with divinylbenzene monomers is realized making use of the concentration difference of moisture repel and vinyl ester is added to carry out a second coating, and the magnetic microspheres are prepared by free radical polymerization. After full alcoholysis reaction, large quantity of active functional groups appear on the surface of the magnetic microspheres, which the thiophilic magnetic composite microsphere is formed by Michael addition reaction and grafting thiophilic petunidin such as sulfhydryl-nicotinic acid, etc., thus realizing specific recognition and separation for human immunoglobulin.

Description

technical field [0001] The patent relates to a preparation method of thionophilic magnetic composite microspheres and a method for separating immunoglobulin G (IgG) by using thionophilic magnetic composite microspheres. Background technique [0002] The rise of nanotechnology has opened up a new field for the research and preparation of biological materials. Magnetic microsphere technology has the characteristics of high efficiency, rapidity, high sensitivity, and simple process. As a detection and separation method with great potential, it has been widely used in bioseparation and biodetection. Nano magnetic balls can detect 10 -18 The concentration range of M is much higher than the limit detection concentration of common chromatographic techniques (10 -12 M). The Norwegian company Dynal has developed a series of commercial products Dynabeads, which has become an important detection tool in the field of biology, but its particle size is relatively large, mainly using ma...

AI Technical Summary

Problems solved by technology

The thiolophilic group in T gel is the core of such high performance. The thiolophilic group is immobilized on the support, which can specifically affinity immunoglobulin G, so as to realize the separation of globulin, but the separation time is longer , the cost is high, especially when T gel is used for separation, it needs to be in a buffer environment with high salt concentration, which leads to easy precipitation of immunoglobulin G and a large loss of biological activity

Moreover, using chromatographic technology, the equipment is complicated, the steps are cumbersome, and the amount of sample processing is small, resulting in higher cost of the final product.