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Preparation method of hyaluronic acid small fragment plaster for promoting the wound healing

A technology of hyaluronic acid and wound healing, which is applied in the direction of medical formula, medical preparations containing active ingredients, and ointment delivery, etc. It can solve the problems of clinical surgical wound treatment and other problems, and achieve low cost, simple method, and strong wound healing Effect

Inactive Publication Date: 2008-10-15
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, once the potential of o-HA to treat wounds is developed, it will have a huge impact on clinical surgical wound management

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Preparation of small hyaluronic acid oligosaccharides (o-HA): purification and molecular weight identification.

[0021] Reference paper: (Gao F, et al. Preparation and characterization of hyaluronanoligosaccharides for angiogenesis study. J Biomed Mater Res 2006; 78(2): 385-392.).

[0022] 1) Dissolve 500mg of hyaluronic acid in 5ml of 1mol / L sodium chloride, add 20mg of bovine testicular hyaluronidase (500U / mg) for enzymolysis at 37°C, and after 16 hours, soak in boiling water for 10 minutes to remove bovine testicular hyaluronic acid Acidase, to obtain small fragment oligosaccharides of hyaluronic acid (o-HA)

[0023] 2) Centrifuge at 1455G relative centrifugal force for 5 minutes, take supernatant and pass through Bio-Gel P10 gel column (column size: 1.5×100 cm; eluent: 1mol / L sodium chloride containing 10% (v / v) ethanol; Flow rate: 0.5ml / min) collect 1 tube every 6 minutes, 100 tubes in total.

[0024] 3) Carbazole method was used to detect the content of uron...

Embodiment 2

[0032] 1. Prepare hyaluronic acid small fragment oligosaccharides (o-HA) according to the method in Example 1.

[0033] 2. Take 0.56g of glyceryl monostearate, 0.3g of ozokerite, and 0.3g of white petrolatum, heat and dissolve in an 80°C water bath, add 1.965g of liquid paraffin, and keep at 80°C as the oil phase.

[0034] 3. Take 7.5 mg of ethyl p-hydroxybenzoate, 25 mg of OP emulsifier, 8050 mg of Span-8050 mg, 15 mg of small fragment oligosaccharides of hyaluronic acid, and 1.7775 g of distilled water as the water phase, and keep it warm at 70-80°C for later use.

[0035] 4. Slowly add the water phase to the oil phase, and keep stirring in the same direction while adding, until the emulsification is completed and condensed, and the product is ready. Finally, a 5g ointment is obtained.

[0036] The hyaluronic acid small fragment ointment prepared according to the method of Example 2 was applied to the wound on the back of the wound healing model mouse, and the local neovasc...

Embodiment 3

[0038] 1. Preparation of hyaluronic acid small fragment oligosaccharides (o-HA).

[0039] 2. Take 0.35g of glyceryl monostearate, 0.175g of ozokerite, and 0.175g of white petrolatum, heat and dissolve in an 80°C water bath, add 2.425g of liquid paraffin, and keep at 80°C as the oil phase.

[0040] 3. Take 75 mg of ethyl p-hydroxybenzoate, 75 mg of OP emulsifier, 175 mg of Span-80, 10 mg of hyaluronic acid small fragment oligosaccharides, and 1.540 g of distilled water as the water phase, and keep it warm at 70-80°C for later use.

[0041] 4. Slowly add the water phase to the oil phase, and keep stirring in the same direction while adding, until the emulsification is completed and condensed, and the product is ready. Finally, a 5g ointment is obtained.

[0042] The hyaluronic acid small fragment ointment prepared according to the method of Example 3 was applied to the wound on the back of the wound healing model mouse, and the local neovascularization of the wound and the woun...

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PUM

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Abstract

The invention relates to a method for preparing hyaluronan fragment ointment for promoting wound healing. The preparation method comprises the steps of: preparing oligosaccharides of hyaluronan, identifying, separating, and purifying to obtain 4-20mers of oligosaccharides of hyaluronan fragment (o-HA); mixing glycerin monostearate, ceresin and white vaseline, melting, adding liquid paraffin, and keeping at 80 DEG C to obtain oil phase; mixing 4-20mers of o-HA, ethyl p-hydroxybenzoate, OP emulsifying agent and Span-80, adding distilled water, and keeping at 70-80 DEG C to obtain water phase; adding water phase into oil phase while stirring until emulsification completes, and condensing to obtain o-HA ointment. The o-HA ointment can effectively delay the local release of o-HA at the wound site, promote local vascularization at the wound site, and promote wound healing.

Description

technical field [0001] The invention relates to a preparation method of a small hyaluronic acid fragment (Hyaluronan oligosaccharides, o-HA) ointment, which is used for promoting wound healing. Background technique [0002] Tissue damage and repair is currently a hot topic in the field of surgery. Clinically, repair obstacles are mainly manifested as slow-healing skin wounds, such as diabetic ulcers, calf ulcers, and pressure ulcers. Due to their high incidence and disability rate, become one of the difficult problems in clinical treatment. Delayed wound healing, a common and refractory clinical disease, has been haunting the medical field. How to effectively promote wound healing is a hot issue in today's medicine. [0003] With the deepening of research on the molecular mechanism of wound healing, in recent years, peptide growth factors have been used clinically to treat various soft tissue injuries, and good results have been achieved. A large number of animal experimen...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K31/728A61P17/02
Inventor 高锋
Owner SHANGHAI JIAO TONG UNIV
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