Preparation method of hyaluronic acid small fragment plaster for promoting the wound healing

Abstract

The invention relates to a method for preparing hyaluronan fragment ointment for promoting wound healing. The preparation method comprises the steps of: preparing oligosaccharides of hyaluronan, identifying, separating, and purifying to obtain 4-20mers of oligosaccharides of hyaluronan fragment (o-HA); mixing glycerin monostearate, ceresin and white vaseline, melting, adding liquid paraffin, and keeping at 80 DEG C to obtain oil phase; mixing 4-20mers of o-HA, ethyl p-hydroxybenzoate, OP emulsifying agent and Span-80, adding distilled water, and keeping at 70-80 DEG C to obtain water phase; adding water phase into oil phase while stirring until emulsification completes, and condensing to obtain o-HA ointment. The o-HA ointment can effectively delay the local release of o-HA at the wound site, promote local vascularization at the wound site, and promote wound healing.

Description

technical field

[0001] The invention relates to a preparation method of a small hyaluronic acid fragment (Hyaluronan oligosaccharides, o-HA) ointment, which is used for promoting wound healing. Background technique

[0002] Tissue damage and repair is currently a hot topic in the field of surgery. Clinically, repair obstacles are mainly manifested as slow-healing skin wounds, such as diabetic ulcers, calf ulcers, and pressure ulcers. Due to their high incidence and disability rate, become one of the difficult problems in clinical treatment. Delayed wound healing, a common and refractory clinical disease, has been haunting the medical field. How to effectively promote wound healing is a hot issue in today's medicine.

[0003] With the deepening of research on the molecular mechanism of wound healing, in recent years, peptide growth factors have been used clinically to treat various soft tissue injuries, and good results have been achieved. A large number of animal experimen...