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Preparation method and use of camellin A and camellin B

A technology of camelliaside and shiya tea, which is applied in the field of botanical medicine to achieve the effects of reasonable design, short production cycle and reduced environmental pollution

Inactive Publication Date: 2008-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on the large-scale preparation of camelliaside A and B from Shiya tea.

Method used

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  • Preparation method and use of camellin A and camellin B
  • Preparation method and use of camellin A and camellin B
  • Preparation method and use of camellin A and camellin B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 camelliaside A

[0025] see figure 1 250g of Shiya tea was taken, crushed until the particle size was below 10mm, placed in a 5000ml round bottom flask, added 3000ml of 80% methanol for reflux extraction for 1 hour, filtered and then added 80% methanol 2000ml for reflux extraction for 1 hour, and combined the two The extract was concentrated to 500ml, extracted twice with 500ml ethyl acetate, and the water layer was further concentrated to 250ml, placed at a temperature of 10°C for crystallization for 2 hours, filtered to obtain yellow total flavonoids of Shiya tea, and the total The flavonoids were crystallized twice with 95% ethanol and 50% ethanol successively to obtain camelliaside A with a purity of 93.6% and a yield of 8.6%.

Embodiment 2

[0026] The preparation of embodiment 2 camelliaside A

[0027] Take 200g of Shiya tea, crush it until the particle size is below 5mm, put it in a 3000ml round bottom flask, add 1600ml of 50% ethanol for percolation extraction, extract for 7 days for the first time, and extract for 3 days after adding solvent for the second time, Combine the two extracts, recover ethanol and concentrate to 600ml, add an equal amount of ethyl acetate to extract and degrease, continue to concentrate the water phase to 300ml, place it at room temperature for crystallization for 1 hour, and filter to obtain yellow total flavonoids of Shiya tea. The flavonoids were crystallized twice with methanol and 80% methanol successively to obtain catechin A with a purity of 91.8% and a yield of 9.5%.

Embodiment 3

[0028] The preparation of embodiment 3 camelliaside A

[0029] Take 300g of Shiya tea, crush it until the particle size is less than 5mm, put it in a 5000ml round bottom flask, add 3000ml of methanol for ultrasonic extraction, extract for 40 minutes for the first time, add solvent for the second time, extract for 20 minutes, and combine twice The extract was concentrated after recovery of methanol to obtain an extract, which was suspended in 600ml of water, added an equal amount of ethyl acetate for extraction and degreasing, and the water phase was further concentrated to 300ml, placed at room temperature for crystallization for 1 hour, and filtered to obtain a yellow The total flavonoids of Shiya tea were crystallized twice with ethanol and 70% ethanol successively to obtain camelliaside A with a purity of 92.8% and a yield of 8.8%.

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Abstract

The invention provides a method for making high-purity camellin A and camellin B. The method is as follows: Adinandra nitida is used as a raw material and is extracted by a hydroalcoholic solvent system; then, the raw material is extracted and degreased by a semipolar organic solvent after depressurized concentration; water-phase continuous concentration of Adinandra nitida total flavone is carried out, and the total flavone is recrystallized to obtain camellin A through a hydroalcoholic system; finally, after the total flavone is turned into camellin B from camellin A by potassium carbonate, camellin B is obtained through ion exchange chromatography or recrystallization directly by the hydroalcoholic system. The invention has reasonable design, simple manufacturing process, low production cost and high yield and purity, and can obtain high-purity camellin A and camellin B through adopting recrystallization method after liquid-liquid extraction; therefore, the invention, omitting the commonly used column chromatography method and reducing the consumption of organic solvent and energy consumption to reduce environmental pollution, is suitable for industrial production. Pharmacological study shows that the method has outstanding protective action on H9C2 cardiac muscle cell line anoxic hypoxia reoxygenation model damage and can be used in curing cardiovascular system disease.

Description

technical field [0001] The invention belongs to the field of herbal medicines, and in particular relates to a preparation method and application of high-purity camelliaside A and B produced from Shiya tea. Background technique [0002] The scientific name of Shiya tea is Adinandra nitida Merr.ex Li. It is a kind of natural and wild precious tea produced in Guangxi. Shiya tea is rich in amino acids, mineral elements, and tea polyphenols. In addition, the total flavonoid content is as high as 28.4%, which is significantly higher than that of ordinary tea. In addition to the effects of ordinary teas for promoting body fluids, quenching thirst, and refreshing the mind, Shiya tea also has the effects of clearing away heat and relieving summer heat, anti-tumor, hemostasis, and lowering blood pressure. Studies have shown that flavonoids in Shiya tea have important physiological activities, of which camelliaside A and B are the main components. However, there is no research report...

Claims

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Application Information

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IPC IPC(8): C07H17/07A61K31/7048A61P9/10A61P9/00
Inventor 甘礼社吴理茂周长新
Owner ZHEJIANG UNIV
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