Camellia effective ingredients and extracting method and use thereof

A technology of active ingredients and extraction methods, applied in the field of production and processing of camellia active ingredient preparations, to achieve clear composition, definite curative effect, and wide-ranging effects

Inactive Publication Date: 2008-11-12
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has not been reported that camellia extract is used for lo

Method used

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  • Camellia effective ingredients and extracting method and use thereof
  • Camellia effective ingredients and extracting method and use thereof
  • Camellia effective ingredients and extracting method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] According to the active ingredient preparation method 1

[0019] (1) Take 10kg of dried camellia to remove impurities and dust.

[0020] (2) Add medicinal materials and 20 times the amount of 70% ethanol into the traditional Chinese medicine extraction tank, and heat and reflux for extraction three times, respectively, for 2 hours, 1.5 hours, and 1 hour.

[0021] (3) Combine the three extracts, concentrate to 20L, load the sample on the non-polar macroporous resin column, and use water (5V R ), 10% ethanol (4V R ), 70% ethanol (8V R ), 95% ethanol (4V R ) Gradient elution. The 10% ethanol eluate and the 70% ethanol eluate are mixed according to the ratio of 1:1 to obtain the effective extract of camellia. The total flavonoid content is 21% and the total saponin content is It is 16% and the content of phenethanol glycoside is 1.5%.

Example Embodiment

[0022] Example 2

[0023] According to the active ingredient preparation method 2

[0024] (1) Take 6kg of dried whole fern to remove impurities and dust

[0025] (2) Add medicinal materials and 25 times the amount of 75% ethanol into the traditional Chinese medicine extraction tank, heat and reflux for three times, respectively, for 2 hours, 2 hours, and 1 hour.

[0026] (3) Combine the three extracts, concentrate to 10L, and extract four times with equal volumes of cyclohexane, dichloromethane, ethyl acetate, and n-butanol. 60 g of cyclohexane extract, 55 g of dichloromethane extract, 170 g of ethyl acetate extract, and 740 g of n-butanol extract were obtained. The solvent is recovered, and the obtained n-butanol extract is further purified by a macroporous resin, and eluted with water and 75% ethanol, respectively, and the solvent is recovered under reduced pressure to obtain 340 g of 75% ethanol eluate. The 75% ethanol eluate and the ethyl acetate extract are mixed in a ratio ...

Example Embodiment

[0027] Example 3

[0028] According to the active ingredient preparation method 3

[0029] (1) Take 5kg of dried camellia to remove impurities and dust

[0030] (2) Add medicinal materials and 15 times the amount of water into the traditional Chinese medicine extraction tank, heat and boil, and extract twice for 1.5 hours each time.

[0031] (3) Combine the two extracts, concentrate to 10L, add ethanol, adjust the alcohol concentration to 50%, stand at a low temperature at 10°C for 24 hours until the precipitation is complete, filter again, and recover the ethanol from the filtrate until there is no alcohol smell.

[0032] (4) Load the supernatant on the non-polar macroporous resin column, and use water (5V R ), 15% ethanol (4V R ), 60% ethanol (6V R ), 90% ethanol (4V R ) Gradient elution, discard the water wash, and mix the 20% ethanol eluate and 60% ethanol eluate in the ratio of 1:1 to the equivalent crude drug quantity to obtain the effective extract of camellia, in which the...

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Abstract

The invention provides an extracting method, comprising a water extraction method, an alcohol extraction, a macroporous resin purification method and a solvent extraction method. By adopting the method, the contents of main effective constituents of the obtained extractive are: 1 to 25 percent of TFA, 1 to 20 percent of total Saponins and 1 to 3 percent of phenethyl alcohol glycoside. The invention also provides a method for making the appropriate preparation formulation by using the camellia directly or mixing the solid or liquid of the preparation and the like pharmaceutically in general. The effective constituents of the camellia and the preparation thereof have the functions of reducing blood sugar and lipid and protecting the gastric mucosa, etc. The extracting method has simple implementation and low cost, extracts sufficiently and is suitable for the industrial production; and the extractive has evident activity, precise healing effect and does not have the toxic and side effect.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and relates to an active ingredient of camellia japonica, an extraction method and application thereof, and a production and processing method of the active ingredient preparation of camellia japonica. Background technique: [0002] Camellia sinensis (Camaellia sinensis) is the flower of the Camellia family, the genus Camellia. It is cultivated on a large scale in southern my country and has a long history of cultivation. Camellia is rich in flavonoids (mainly kaempferol-type flavonoids, quercetin-type flavonoids, flavans), saponins (tea saponin type) and phenylethanol glycosides. Pharmacological experiments show that camellia extract has good effects of lowering blood sugar, lowering blood fat, protecting gastric mucosa, and anti-oxidation. However, it has not been reported that camellia extract is used for lowering blood sugar, lowering blood fat, and protecting gastric mucosa. Invention ...

Claims

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Application Information

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IPC IPC(8): A61K36/82A23L1/29A61P3/10A61P3/06A61P1/04A23L33/00
Inventor 李宁肖皖肖海涛杜超吴恩华庞挺
Owner SHENYANG PHARMA UNIVERSITY
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