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Camellia effective ingredients and extracting method and use thereof

A technology of active ingredients and extraction methods, applied in the field of production and processing of camellia active ingredient preparations, to achieve clear composition, definite curative effect, and wide-ranging effects

Inactive Publication Date: 2008-11-12
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been reported that camellia extract is used for lowering blood sugar, lowering blood fat, and protecting gastric mucosa.

Method used

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  • Camellia effective ingredients and extracting method and use thereof
  • Camellia effective ingredients and extracting method and use thereof
  • Camellia effective ingredients and extracting method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] According to the active ingredient preparation method 1

[0019] (1) Get 10kg of dry camellia, remove impurities and dust.

[0020] (2) Add medicinal materials and 20 times the amount of 70% ethanol in the traditional Chinese medicine extraction tank, heat and reflux extraction three times, respectively for 2 hours, 1.5 hours, and 1 hour.

[0021] (3) Combine the three extracts, concentrate to 20L, load the sample to the non-polar macroporous resin column, and use water (5V R ), 10% ethanol (4V R ), 70% ethanol (8V R ), 95% ethanol (4V R ) gradient elution, get water washings, discard 10% ethanol eluate, 70% ethanol eluate and mix according to the ratio of 1:1 equivalent crude drug amount, to get Camellia japonica effective extract, wherein the total flavonoid content is 21%, the total saponin content is 16%, and the phenylethanol glycoside content is 1.5%.

Embodiment 2

[0023] According to the active ingredient preparation method 2

[0024] (1) Take 6kg of dry whole fern, remove impurities and dust

[0025] (2) Add medicinal materials and 25 times the amount of 75% ethanol in the traditional Chinese medicine extraction tank, heat and reflux extraction three times, respectively for 2 hours, 2 hours, and 1 hour.

[0026] (3) The three extracts were combined, concentrated to 10 L, and extracted four times with an equal volume of cyclohexane, dichloromethane, ethyl acetate, and n-butanol. 60 g of cyclohexane extract, 55 g of dichloromethane extract, 170 g of ethyl acetate extract, and 740 g of n-butanol extract were obtained. The solvent was recovered, and the obtained n-butanol extract was further purified by macroporous resin, and eluted with water and 75% ethanol respectively, and the solvent was recovered under reduced pressure to obtain 340 g of 75% ethanol eluate. The 75% ethanol eluate is mixed with the ethyl acetate extract at a ratio o...

Embodiment 3

[0028] According to the active ingredient preparation method 3

[0029] (1) Get 5kg of dried camellia, remove impurities and dust

[0030] (2) Add medicinal materials and 15 times the amount of water in the traditional Chinese medicine extraction tank, heat to boil, and extract twice, each time for 1.5 hours.

[0031] (3) Combine the two extracts, concentrate to 10L, add ethanol, adjust the alcohol concentration to 50%, let stand at 10°C for 24 hours until the precipitation is complete, filter again, and recover the ethanol from the filtrate until it has no alcohol smell.

[0032] (4) The supernatant is loaded onto the non-polar macroporous resin column, and water (5V R ), 15% ethanol (4V R ), 60% ethanol (6V R ), 90% ethanol (4V R ) gradient elution, the washings obtained were discarded, and the 20% ethanol eluate and the 60% ethanol eluate were mixed according to the ratio of 1:1 of the equivalent crude drug amount to obtain the effective extract of Camellia sinensis, wh...

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Abstract

The invention provides an extracting method, comprising a water extraction method, an alcohol extraction, a macroporous resin purification method and a solvent extraction method. By adopting the method, the contents of main effective constituents of the obtained extractive are: 1 to 25 percent of TFA, 1 to 20 percent of total Saponins and 1 to 3 percent of phenethyl alcohol glycoside. The invention also provides a method for making the appropriate preparation formulation by using the camellia directly or mixing the solid or liquid of the preparation and the like pharmaceutically in general. The effective constituents of the camellia and the preparation thereof have the functions of reducing blood sugar and lipid and protecting the gastric mucosa, etc. The extracting method has simple implementation and low cost, extracts sufficiently and is suitable for the industrial production; and the extractive has evident activity, precise healing effect and does not have the toxic and side effect.

Description

Technical field: [0001] The invention belongs to the technical field of medicine, and relates to an active ingredient of camellia japonica, an extraction method and application thereof, and a production and processing method of the active ingredient preparation of camellia japonica. Background technique: [0002] Camellia sinensis (Camaellia sinensis) is the flower of the Camellia family, the genus Camellia. It is cultivated on a large scale in southern my country and has a long history of cultivation. Camellia is rich in flavonoids (mainly kaempferol-type flavonoids, quercetin-type flavonoids, flavans), saponins (tea saponin type) and phenylethanol glycosides. Pharmacological experiments show that camellia extract has good effects of lowering blood sugar, lowering blood fat, protecting gastric mucosa, and anti-oxidation. However, it has not been reported that camellia extract is used for lowering blood sugar, lowering blood fat, and protecting gastric mucosa. Invention ...

Claims

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Application Information

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IPC IPC(8): A61K36/82A23L1/29A61P3/10A61P3/06A61P1/04A23L33/00
Inventor 李宁肖皖肖海涛杜超吴恩华庞挺
Owner SHENYANG PHARMA UNIVERSITY
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