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Hyphomycetes mite-killing preparation

A technology of preparation of Metarhizium anisopliae of scarabia, which is applied in the direction of acaricides, biocides, fungi, etc., to achieve the effects of reducing use, improving safety, and good control effect

Inactive Publication Date: 2011-03-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the adult mites are very small and very active, the bioassay technology for the screening of highly effective acaricidal strains is very difficult, and there are few related research reports, which are still in the initial exploration stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, preservation and cultivation of mycospore biocontrol fungi

[0019] Hyphospora biocontrol fungi such as Metarhizium anisopliae ZJUIM24-562 and Beauveria bassiana ZJUIM17-340 were stored in a -72°C ultra-low temperature refrigerator in the form of dry conidia powder mixed with sterilized white sand. The preserved strains were all activated on Sabouraud's medium SDAY plates at 25°C for about 7 days for the production of spore powder. The SDAY formula (W / W) of Sabouraud medium is: 4% glucose, 1% peptone, 1% yeast and 2% agar.

Embodiment 2

[0020] Embodiment 2, the production of high-purity conidia powder

[0021] The production of the high-purity conidium powder of hyphosporium biocontrol fungi adopts a kind of vertical multi-layer sporulation box (patent publication number CN 1710059A), and the liquid-solid two-phase fermentation method ( Patent Publication No. CN 1415736) production, specifically according to the following steps:

[0022] 1) The mycelia liquid is obtained by carrying out liquid-phase fermentation of the hyphospora high-efficiency acaricidal strain using Sabouraud's culture solution or wheat bran extract.

[0023] 2) Choose low-value rice, washed and drained, steam sterilized and matured to 5-6 mature, and then used as solid-phase fermentation substrate after cooling.

[0024] 3) Mix the mycelia liquid obtained from the above liquid phase fermentation into the above solid phase fermentation substrate at a ratio of 8-12 liters / 100 kg of rice, and stir evenly with a machine to form the inoculation...

Embodiment 3

[0028] Embodiment 3, the determination of the acaricidal activity of hyphosporium biocontrol fungi

[0029] The high-purity spore powder obtained in Example 2 was diluted with 0.02% Tween 80 solution into high, medium and low three spore dose spore suspensions, and the aqueous solution containing only 0.02% Tween 80 was used as a blank control. The test adopts the Potter spray tower method in order from low to high, at 0.7kg / cm 2 Spray 2ml of the aqueous spore suspension onto the blades of the stage with a diameter of 11cm and loaded with adult spider mites or mite eggs. The actual inoculum dose under each concentration is collected with a cover glass (20cm × 20cm) placed flat on the side of the leaf. 2 / field of view) the number of spores, so that the amount of spores deposited on the mite body and its leaves is standardized as the number of spores / mm 2 . After inoculation, place the female adult mites or mite eggs in an incubator at 25°C 12L:12D for 7 to 9 days of observ...

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Abstract

The invention discloses a high efficiency capillitium fungi acaricide which can effectively control phytophagous harmful mites, reduce the usage of chemical pesticides and improve the safety of agricultural products. The scarab Metarrhizium anisopliae ZJUIM24-562S strain and sorosphere Beauveria bassiana ZJUIM17-340 strain of the invention are preserved on February 25, 2008 and the preserve codesseparately are CGMCC No.2375 and CGMCC No.2374. The preparation comprises capillitium fungi conidium, mineral oil, emulsifying agent, uv-protector and stabilizer. The results of indoor and outdoor tests show that capillitium fungi acaricide has ideal extermination activity and field control efficiency for various phytophagous harmful mites. Therefore, the high efficiency capillitium fungi acaricide will fill the study knowledge and technical gaps of fungi acaricide in China.

Description

technical field [0001] The invention relates to a kind of hyphosporium fungus acaricidal preparation for preventing and controlling phytophagous harmful mites. Background technique [0002] Tetranychus generally refer to the species of Tetranychidae, which belong to herbivorous mites, and are generally harmful mites to polyphagous crops and economic plants, with many types and wide distribution. The species of Tetranychus endangered in my country's cotton area mainly include Tetranychus cinnabarinus, Tetranychus truncation, Tetranychus urticae, Tetranychus dunhuang and Tetranychus Turkestan. The most serious herbivorous spider mite in the vast citrus gardens is Panonychus citrus, which has risen significantly in recent years to become the main pest of citrus. Except citrus, it also harms plants such as pear, peach, persimmon, jujube, tea, mulberry, osmanthus, weeping willow, rose and holly. Tetranychus usually use adult mites, nymphs, and young mites to suck juice on the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/02C12R1/645
Inventor 施卫兵冯明光
Owner ZHEJIANG UNIV