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Anti influenza use of main chemical component and active component in scabriculous herbamoslae

A technology of fenugreek and fenugreek ethyl acetate, which is applied to medical preparations containing active ingredients, plant/algae/fungus/moss components, anti-toxins, etc., and can solve problems such as high price, side effects, and clinical application limitations

Inactive Publication Date: 2013-05-08
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the discovery of neuraminidase (neuraminidase, NA) inhibitors, the treatment of influenza was limited to amantadine / rimantadine and virus vaccines, but its shortcomings are (1) because the effectiveness of influenza vaccines is based on the vaccine On the basis of the similarity of the virus strains to the influenza virus strains that will be popular or exist in the environment, the high variability of influenza virus antigens greatly reduces the accuracy of people's prediction of influenza virus outbreaks, which makes conventional influenza vaccines effective for prevention Effectiveness of influenza outbreaks and epidemics is severely compromised and helpless against emerging unforeseen influenza strains
(2) Influenza virus M2 protein inhibitors amantadine and rimantadine are only effective against type A influenza virus, and drug-resistant virus strains and relatively serious central nervous system side effects will appear soon, which limits the clinical application of these drugs extremely restrictive
However, due to technical reasons, the preparation process of the drug is complicated, the production cost is high, and the price is expensive, which limits its wide application.
In China, vaccine research has been carried out in the prevention and treatment of influenza, and some progress has been made, but in terms of drug research, there are no effective drug research results.

Method used

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  • Anti influenza use of main chemical component and active component in scabriculous herbamoslae
  • Anti influenza use of main chemical component and active component in scabriculous herbamoslae
  • Anti influenza use of main chemical component and active component in scabriculous herbamoslae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: Establish neuraminidase inhibitor screening model (references: Potier, M., L.Mameli, M.Belislem, L.Dallaire, and S.B.Melanxon. Fluorometric assay of neuraminidasewith a sodium (4-methylumbelliferyla-D-N -acetylneuraminate)substrate.Anal.Biochem., 1979,94:287-296.), through the evaluation of the neuraminidase inhibitory activity of 100,000 samples in the sample bank, it was found that the water extract of A. Acidase inhibitory activity, its IC 50 1 ~ 4ug / ml.

Embodiment 2

[0062] Example 2: After drying and pulverizing 30kg of the whole herb of A. chinensis, extract 3 times with 85% ethanol (2h, 1h×2), combine the extracts, evaporate to dryness under reduced pressure, and obtain the total ethanol extract (ER-ethanol total Extract, 1.675kg), be dissolved in water afterwards, obtain sherwood oil part (ER-petroleum ether layer, 0.012kg) with sherwood oil extraction, remaining part obtains ethyl acetate part respectively with ethyl acetate, n-butanol extraction respectively ( ER-ethyl acetate layer, 0.040kg) and n-butanol part (ER-n-butanol layer, 0.057kg), and finally the water layer part (ER-water layer, 0.725kg).

Embodiment 3

[0064] Petroleum ether is further separated to obtain betulinic acid, oleanolic acid and ursolic acid.

[0065] The ethyl acetate fraction (235.3 g) was further separated to obtain apigenin (55 mg), luteolin (113 mg), apiin (15 mg), luteolin (35 mg), and luteolin- 3'-glucuronic acid methyl ester (luteolin-3'-glycuronic methyl ester, 19mg), and lignans (27mg) and other components. The separation and purification results of Example 3 show that 3 triterpenoids, 5 flavonoids and 1 lignan are the main chemical components of the traditional Chinese medicine.

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Abstract

The invention relates to a method for preparing effective components and main active constituents of a Chinese medicinal herb Elsholtzia rugulosa Hems I, a pharmaceutical composition containing the effective components and the main active constituents, and application of the active constituents to preventing and treating influenza disease. The invention is characterized in that the effective components extracted from the Elsholtzia rugulosa Herms I and the active constituents separated from the Elsholtzia rugulosa Herms I have inhibitory action on neuraminidase and has anti-oxidation and anti-inflammation effects and immunization.

Description

technical field [0001] The invention relates to the preparation method of the main chemical components and effective components of the traditional Chinese medicine Azalea chinensis, the new pharmacological action and its application in the prevention and treatment of influenza diseases, belonging to the technical field of traditional Chinese medicine. Background technique [0002] Influenza is caused by influenza virus and is one of the respiratory infectious diseases that threaten human health the most. The mortality rate is very high in children, the elderly and high-risk groups, which seriously threatens human health. Influenza viruses belong to the Orthomyxoviridae family, and are divided into three types: A, B, and C according to the difference in viral nucleoprotein and matrix protein epitopes, of which only types A and B can cause large-scale epidemics, and types B and C It can only be pathogenic to humans, and type A can be pathogenic to humans, pigs, horses and pou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/53A61P29/00A61P31/16A61P37/04A61P39/06
Inventor 刘艾林杜冠华秦海林刘波何国荣胡娟娟
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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