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Technique for extracting and separating tea polyphenol from tea-leaf

A technology of tea polyphenols and a new process is applied in the field of extraction and separation of tea polyphenols, which can solve the problems of high waste water treatment cost, destruction of effective components, and high operating energy consumption, and achieves the reduction of energy consumption, the improvement of product quality, and the low energy consumption. Effect

Inactive Publication Date: 2009-03-18
CHUNTIAN BIOTECH DEV XIANGXI AUTONOMOUS COUNTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a new process for extracting and separating tea polyphenols from tea leaves. The problem of high cost of polysaccharide wastewater treatment

Method used

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  • Technique for extracting and separating tea polyphenol from tea-leaf
  • Technique for extracting and separating tea polyphenol from tea-leaf

Examples

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Effect test

Embodiment 1

[0029] Example 1: A new process for extracting and separating tea polyphenols from tea leaves

[0030] as attached figure 1 As shown, 1Kg of tea leaves, after being treated with the treatment steps, is wetted with 500ml of water, placed in the percolation tank 1, and the ethyl acetate is heated to 50°C by the preheater 2 at a flow rate of 4L / h through a peristaltic pump. Then it is sent into the percolation tank to extract tea polyphenols by percolation, and the continuous effluent passes through the plate falling film concentrator 3 to recover the solvent, and the recovered solvent obtained continues to be used for percolation extraction. Randomly detect EGCG (TLC method) at the outlet of the percolation tank during the process, until no EGCG spots appear, collect the concentrated solution and place it in a 3000ml stirring tank to recover the solvent until it is clean, add 500ml of n-hexane and stir to extract and remove the fat-soluble substances. Get tea polyphenol product...

Embodiment 2

[0031] Example 2: A new process for extracting and separating tea polyphenols from tea leaves

[0032] as attached figure 2 As shown, 1Kg of tea leaves is wetted with 1000ml of water, placed in the percolation tank 1, and the ethyl acetate containing n-hexane with a volume concentration of 15% is heated to At 50°C, enter the percolation tank to extract tea polyphenols by percolation, the extract flows through the adsorption column 4, and the polyamide absorbs tea polyphenols, and the effluent returns to the percolation tank to form a circular extraction. During the process, randomly detect EGCG at the outlet of the percolation tank (TLC method), and stop the cycle operation until there are no EGCG spots. Continuously inject water from the bottom of the adsorption column 4, about 5 times the column volume, until there is no caffeine and ethyl acetate-petroleum ether (extractant) residue in the effluent, drain the water, analyze and concentrate with 95% ethanol to obtain tea p...

Embodiment 3

[0033] Example 3: A new process for extracting and separating tea polyphenols from tea leaves

[0034] Wet 1Kg of tea leaves with 200ml of water, place them in a percolation tank, and heat 10% ethyl acetate containing n-hexane through a peristaltic pump to 50°C through a preheater at a flow rate of 4L / h, and then send Enter the percolation tank to extract tea polyphenols by percolation, and the continuous effluent recovers the solvent through the plate falling film concentrator, and the recovered solvent is continued to be used for percolation extraction. Randomly detect EGCG (TLC method) at the outlet of the percolation tank during the process, until no EGCG spots appear, collect the concentrated solution and place it in a 3000ml stirring tank to recover the solvent until it is clean, add 500ml of petroleum ether and stir to remove the fat-soluble substances, to obtain Tea polyphenol products.

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Abstract

The invention discloses a novel process for extracting and separating tea phenol, which comprises the steps of extracting, liquid-liquid partition chromatography, and column chromatography integrated separation or the steps of extracting, liquid-liquid partition chromatography, and concentration integrated separation. The process comprises the following steps of: firstly, mixing water and tea at the mass ratio of between 0.2 and 1 to 1 uniformly, using extractant to extract the solution to obtain extraction liquid; then performing column chromatography or concentration drying to obtain tea phenol products with various specifications. During the extraction process, water-soluble impurities such as tea polysaccharide are retained in the tea and no tea polysaccharide waste water is produced. The tea polysaccharide, protein, fibrin, microelements and other compositions are retained in dried tea dregs after extraction, which can be used as good feed additives. The process can adopt integrated separation so as to improve the stability and the degree of automation greatly and reduce the number of workers and the labor intensity.

Description

technical field [0001] The invention relates to a new process for extracting and separating tea polyphenols from tea leaves, which belongs to the field of natural organic chemistry. Background technique [0002] Tea polyphenols (Tea polyphenols, TP) is a major chemical component in tea, is a pure natural compound, its content accounts for about 10% to 30% of the total dry matter of tea. Tea polyphenols are composed of catechins (flavanols), anthocyanins (anthocyanins and anthocyanins), anthocyanins (flavonoids and flavonols), phenolic acids and phenolic acids. Tea compounds are the most important, accounting for about 60% to 80% of the total amount of tea polyphenols. [0003] Catechins are mainly composed of gallocatechin (Gallocatechin, GC), D, L-catechin (D, L-catechin, C), epicatechin (Epicatechin, EC), catechin gallate ( Catechingallate, CG), gallocatechin gallate (Gallocatechin gallate, GCG), epigallocatechin gallate (Epigallocatechin gallate, EGCG), epicatechin gall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G17/00C07D311/62C07D311/26
Inventor 刘强张蕾
Owner CHUNTIAN BIOTECH DEV XIANGXI AUTONOMOUS COUNTY
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