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Preparation method of active components in root-bark tree peony by in-phase leaching and sub-item preparation

A technology of paeonol extraction and extraction, which is applied in the field of extracting active ingredients in Chinese herbal medicine, can solve the problems of outdated and cumbersome technical means, waste of raw paeonol, and low production efficiency, and achieve the effects of reducing costs, reducing reagent consumption, and low cost

Inactive Publication Date: 2011-06-22
ANHUI CHINATURE BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the development and application of paeonol is limited to one of the active ingredients, the technical means used are relatively old and cumbersome, the production efficiency is low, the energy consumption is large, and the raw material paeonol is wasted, which limits the use in industry. Application in production, and there is no report so far from the method of comprehensively extracting three active substances of paeonol, paeonol, paeonol and paeonol total glycosides simultaneously

Method used

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  • Preparation method of active components in root-bark tree peony by in-phase leaching and sub-item preparation
  • Preparation method of active components in root-bark tree peony by in-phase leaching and sub-item preparation
  • Preparation method of active components in root-bark tree peony by in-phase leaching and sub-item preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 500g of paeonol crude drug, wash and dry, add 5L of water, and extract with warm water at 35°C for 8 hours. Filter to obtain medicinal residues and extracts. The extract (filtrate) uses an ultrafiltration membrane with a molecular weight cut-off of 10kDa, and is separated by tangential flow ultrafiltration at a pressure of 30psi and a temperature of 35°C to obtain a retentate containing the macromolecular paeonol polysaccharide and a permeate containing the small molecule total paeonol glycosides. over liquid. The paeonol polysaccharide retentate was precipitated with 3 times the volume of 70% ethanol for 12 hours, and the precipitate was collected and freeze-dried to obtain the paeonol polysaccharide fraction. The total paeoniflorin permeate liquid is concentrated under reduced pressure, the distillate is retained, the concentrated liquid is put on the macroporous resin column AB-8 (3.5g paeoniflorin / kg wet resin), and first washed with water for 6 column volumes...

Embodiment 2

[0030]Take 500g of paeonol crude drug, wash and dry, add 5L of water, and extract with warm water at 35°C for 8 hours. Filter to obtain medicinal residues and extracts. The extract (filtrate) uses an ultrafiltration membrane with a molecular weight cut-off of 30kDa, and is separated by tangential flow ultrafiltration at a pressure of 25psi and a temperature of 30°C to obtain the retentate containing the macromolecular paeonol polysaccharide and the permeate containing the small molecule total paeonol glycosides. over liquid. The paeonol polysaccharide retentate was precipitated with 3 times the volume of 80% ethanol for 12 hours, and the precipitate was collected and freeze-dried to obtain the paeonol polysaccharide fraction. The paeony total glycosides permeated liquid was concentrated under reduced pressure and then applied to the macroporous resin column AB-8 (3.5 mg paeoniflorin / g wet resin). First, rinse 6BV with water to remove impurities, then elute 3BV with 50% ethano...

Embodiment 3

[0032] (1) The mensuration of paeonol purity in embodiment one (ultraviolet detection-high performance liquid chromatography)

[0033] Chromatographic conditions: column Restek pinnacle C 18 Column (4.6 * 250mm, 5 μ m); Detector Waters2487 ultraviolet detector (U.S. Waters Company); Waters515 chromatography pump (U.S. Waters Company); Detection wavelength 274nm; Mobile phase methanol: water=45:55 (v / v); 1mL / min; injection volume 20μL.

[0034] Preparation of the test solution: Accurately weigh an appropriate amount of the paeonol sample and standard substance in Example 1 of the same quality, dissolve them in a volumetric flask with methanol, shake well, and obtain.

[0035] Determination method: Precisely draw sample solution and standard solution respectively, inject liquid chromatograph, calculate and measure by peak area normalization method, see results figure 2 .

[0036] Analysis results: figure 2 Among them, the relative retention time of the paeonol peak was 14....

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Abstract

Disclosed is a preparation method of simutanteously extacting and partially preparing active ingredients in cortex moutan; crude cortex moutan is immersed in water under temperature of 25-45 DEG C for 4 hours, then solid phase residue and liquid phase water extract are obtained through separation; the solid phase residue is added with water and immersed in water under room temperature, then paeonol is prepared through water vapor distillation method; the liquid phase water extract is processed with ultrafiltration separation through an ultrafiltration membrane with molecular weight cutoff of 5kDa, 10kDa or 30kDa under the pressure of 25-35psi and the temperature of 25-45 DEG C, so as to obtain retentate liquid and permeate liquid respectively; Cortex moutan polysaccharide is prepared fromretentate liquid through alcohol sedimentation method; total glucosides of mudan cortex is prepared from permeate through macroporous resin adsorption separation. The preparation method comprehensively extracts paeonol, cortex moutan polysaccharide and total glucosides of mudan cortex from Chinese traditional medicine cortex moutan and makes full use of the herbal materials; the purity of prepared cortex moutan polysaccharide is 80-84%; the purity of total glucosides of mudan cortex is 71.8-82.3%; wherein, the content of paeoniflorin is 41.7-47.5%; the paeonol is purified to have the purity of 95-99%.

Description

1. Technical field [0001] The invention relates to a method for extracting active components in Chinese herbal medicines, in particular to a method for synchronous extraction and separate preparation of paeonol, paeonol polysaccharides and paeonol total glycosides in paeonol. 2. Background technology [0002] Traditional Chinese medicine is the treasure of the big family of the Chinese nation. In the 21st century, the upsurge of "green food" and "green medicine" is rising all over the world. It is of great significance and value to develop traditional Chinese medicine and realize the modernization of Chinese medicine. In addition, discovering new drugs from chemical compounds is difficult, costly, long-term, and has obvious toxic and side effects, making the demand for natural drugs in the international market increasing. The traditional Chinese medicine and natural drug industry contains huge market potential and business opportunities. [0003] The traditional Chinese med...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71
Inventor 陈彦孙文秀王子尧祝庆军
Owner ANHUI CHINATURE BIOLOGICAL CO LTD