ELISA detecting method for dioctyl phthalate

A dioctyl phthalate, enzyme-linked immunoassay technology, applied in the field of immunoassay, can solve the problems of high cost, cannot meet the requirements of rapidity, convenience, and accuracy, and achieve high selectivity and sensitivity, stability and stability. Good repeatability and low cost results

Inactive Publication Date: 2009-04-29
JIANGNAN UNIV
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of dioctyl phthalate at home and abroad mainly adopts high performance liquid chromatography (HPLC), gas chromatography (GC), gas-mass spectrometry (GC-MS), liquid-mass spectrometry (LC -MS), etc., although these methods are sensitive, they require expensive instruments and equipment, professional operators, and relatively high requirements for test materials, and further sample pretreatment is required. Convenient and Accurate Requirements
In recent years, studies on the immunoassay method of phthalates have been carried out at home and abroad, but there is no report on the enzyme-linked immunoassay for dioctyl phthalate at home and abroad. In order to make up for this gap, it is necessary to target Establishment of an enzyme-linked immunoassay method for dioctyl phthalate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ELISA detecting method for dioctyl phthalate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0052] specific implementation plan

[0053] The present invention is further illustrated by the following examples.

[0054] 1. Instrument:

[0055] TGL-40B desktop low-speed centrifuge, Shanghai Anting Scientific Instrument Factory;

[0056] KFLOW pure water machine, KFLOW company;

[0057] ZD-9556 horizontal shaker, Taicang Science and Education Equipment Factory;

[0058] Costar 96-well 8×12 detachable microtiter plate, Shanghai Jitai Biotechnology Co., Ltd.;

[0059] MuLtiska Mks microplate reader, Thermo Labsystems;

[0060] Adjustable pipette, Thermo Labsystems;

[0061] Vortex mixer, Shanghai Huxi Instrument Analysis Factory.

[0062] 2. Reagents:

[0063] Horseradish peroxidase-labeled goat anti-rabbit GAR-HRP, Kangcheng Bioengineering Company;

[0064] Tetramethylbenzidine (TMB), Huamei Bioengineering Company;

[0065] All other reagents were of analytical grade.

[0066] 3. Steps

[0067] 1. Synthesis of Immunogen and Coatogen

[0068] Synthetic immunogen ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
linear rangeaaaaaaaaaa
Login to view more

Abstract

The invention relates to an enzyme-linked immunoassay method for dioctylphthalate, and belongs to the technical field of immunodetection. The invention utilizes immunity of synthesized dioctylphthalate immunogen to obtain a polyclonal antibody, and takes the dioctylphthalate as standard and conjugate of dioctylphthalate hapten and OVA as envelope antigen, to establish indirect competitive enzyme-linked immunoassay method for the dioctylphthalate. The invention establishes the indirect competitive ELISA method for the dioctylphthalate, and provides a quick and high-efficiency detecting method for detecting residual of the dioctylphthalate. The method adopts the polyclonal antibody, so the cost is lower and the stability and repetitiveness are good; the sensitivity is 0.01ng/mL, and the linear range is between 0.1 and 19ng/mL; and high specificity and compatibility of an immunoreaction make the ELISA have extremely high selectivity and sensitivity.

Description

technical field [0001] The invention relates to a method for detecting phthalate residues, in particular to an enzyme-linked immunological detection method for dioctyl phthalate, belonging to the technical field of immunological detection. Background technique [0002] Dioctylphthalate (DOP) is a kind of phthalate. Phthalate esters are widely used as plasticizers in the processing and production of plastics, especially in the processing and production of polyvinyl chloride (PVC). Since the phthalate plasticizer and the plastic matrix do not form a covalent bond, but are connected by hydrogen bonds and van der Waals forces, they maintain their independent chemical properties, so when they come into contact with water and oil contained in packaged food , it will dissolve. After research, it has been found that phthalate is an environmental hormone substance, which interferes with the production, release, transfer, metabolism, reaction and elimination of normal hormones in or...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 胥传来徐丽广马伟李灼坤
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap