Method for cultivating nitrosobacteria flora and method for treating wastwater containing ammonia nitrogen

A technology of nitrous bacteria and culture method, applied in biological water/sewage treatment, microorganism-based method, water/sludge/sewage treatment, etc., can solve the problems of long hydraulic retention time, low organic load, insufficient explanation, etc. , to achieve the effect of outstanding effect and simple process

Active Publication Date: 2013-11-20
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these processes have played an important role in the denitrification of sewage, there are still the following problems: ①The total hydraulic retention time of the system is long, and the organic load is low, which increases the capital investment cost; ②Complete nitrification of ammonia nitrogen consumes a large amount of dissolved oxygen, Increased the power cost of aeration and oxygenation; ③ When the organic carbon source used as the electron donor in the denitrification process is insufficient, it needs to be supplemented, which increases the cost of chemical dosing
Although many factors can lead to NO in the nitrification process 2 -N accumulation, but theoretical explanations for this phenomenon are currently insufficient

Method used

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  • Method for cultivating nitrosobacteria flora and method for treating wastwater containing ammonia nitrogen
  • Method for cultivating nitrosobacteria flora and method for treating wastwater containing ammonia nitrogen
  • Method for cultivating nitrosobacteria flora and method for treating wastwater containing ammonia nitrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 enrichment of nitrous bacteria

[0033] use as Figure 4 The device shown is for enrichment, the effective volume of the reactor in the device is 3.5L, the residence time is set to 24h, the reaction temperature is room temperature, aeration and stirring, DO>2.0mg / L, and the pH controller is used to feed 30-100g / L NaHCO 3 Control 7.5≤pH≤9.5.

[0034] The nitrifying bacteria are derived from the activated sludge of the sewage treatment workshop of the oil refinery. Before enriching the nitrous bacteria group, the method of Example 1 in CN200710010383.0 is used to enrich the nitrifying bacteria to obtain a high concentration of nitrous bacteria and nitric acid bacteria. of activated sludge.

[0035] After the nitrifying bacteria are enriched according to the CN200710010383.0 method, the step of cultivating the nitrosifying bacteria is carried out. The influent is prepared according to the method of CN200710010383.0 (the same below), and the COD value is lowe...

Embodiment 2

[0036] Embodiment 2 batch culture expands nitrous bacteria group

[0037] A 10L fermenter was directly used for cultivation, and the self-prepared wastewater mentioned in Example 1 was used as the culture solution. In the case of low nitrite concentration, the form of adding ammonium sulfate in batches is used to provide ammonia nitrogen for the growth of nitrifying bacteria. When the nitrite nitrogen rises enough to affect the growth of nitrifying bacteria, that is, the sludge concentration is no longer When it rises, we pump out about 3 / 4 of the culture solution, settle and separate to obtain the cultured nitrifying bacteria and store it for later use. The remaining 1 / 4 is used as seeds. The growth trend of the flora see figure 2 , the final sludge concentration increased to 2650mg / L, more than 4 times higher than the initial sludge concentration of 538mg / L.

Embodiment 3

[0038] Embodiment 3 Constant Turbidity Culture Nitrite Bacteria

[0039] For the turbidity culture process, see image 3 , A is the nitrifying bacteria cultivation tank, the effective volume is 3.5L, B is the sludge settling tank, C is the effluent collection tank, D is the cultured nitrifying bacteria collection tank. We divide the whole culture process into two phases: proliferation phase and constant turbidity culture phase. The culture medium is self-prepared waste water. In the multiplication stage, the concentration of ammonia nitrogen in the substrate is 3000mg / L, the inoculation amount is 1%, the initial sludge concentration is 592mg / L, aeration and stirring, DO≥2mg / L, and the temperature is kept at room temperature 32°C. After 78 hours of cultivation, the ammonia nitrogen is basically consumed After completion, the sludge concentration in the system increased to 2700mg / L, which was more than 4 times higher than the initial sludge concentration. The constant turbidi...

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Abstract

The invention discloses a culture method of a nitrosomonas sp. flora and a treatment method of waste water containing ammonia nitrogen. The ammonia nitrogen concentration and the pH value of a matrix are increased during an enrichment process, and free NH3 with higher concentration is maintained, thereby gradually achieving the purposes of eliminating nitrobacter sp. and enriching nitrosomonas sp. and obtaining a nitrosomonas sp. flora. Then, the obtained nitrosomonas sp. flora is taken as seeds, the expanded culture is carried out by utilizing self-prepared ammonia nitrogen waste water or high ammonia nitrogen waste water as a culture liquid, the nitrosomonas sp. flora after the expanded culture is inoculated into a biochemical treatment pool for improving the removal efficiency of ammonia nitrogen by biochemical treatment, or the nitrosomonas sp. flora after the expanded culture is directly used for removing the ammonia nitrogen of the ammonia nitrogen waste water, and the optimal proposal is used in the aerobic nitrification stage of the shortcut nitrification-denitrification process for accumulating nitrite nitrogen as an electron acceptor for denitrification directly. The method has simple process and outstanding effect of treating the waste water containing the ammonia nitrogen.

Description

technical field [0001] The invention relates to a method for cultivating nitrous bacteria. The cultured nitrifying bacteria are added to the aeration tank of a sewage treatment plant and directly used for the removal of ammonia nitrogen in domestic sewage or industrial sewage, or directly used in short-range nitrification and denitrification processes. process. Background technique [0002] At present, the biological denitrification of wastewater mainly adopts the activated sludge method. However, due to the slow growth of nitrifying bacteria, in order to avoid their loss and obtain a better denitrification effect, the sludge is often required to stay in the reactor for a long time, that is, a large aeration tank is required, which limits the use of the activated sludge system. processing power. Although nitrifying bacteria exist in almost all biological treatment systems, there are contradictions between the biological characteristics of nitrifying bacteria and traditiona...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F101/38
Inventor 张全高会杰黎元生佟明友唐似茵
Owner CHINA PETROLEUM & CHEM CORP
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