Fluorescent tracing multifunctional decoloring Shewanella engineered decolorationis and construction method thereof

Abstract

The invention discloses a discoloring Shiva engineering bacterium with multiple functions of fluorescent tracing and discoloring and a construction method of the discoloring Shiva engineering bacterium, and aims at providing a genetic engineering bacterium that has high-efficiency discoloring capability of three major dyes including azo, anthraquinone and triphenylmethane as well as the fluorescent tracing function. The invention adopts the technical proposal that combines a foreign fusion gene SPrtpmD' into a discoloring Shiva bacterium S12; wherein, the fusion gene SPrtpmD' is formed by connecting a discoloring enzyme modifier gene tpmD' of the triphenylmethane dye after a NAD(P)H dehydrogenase gene initiator sequence of the discoloring Shiva bacterium S12, and the discoloring enzyme modifier gene tpmD' of the triphenylmethane dye is formed by adding an SD sequence before a 5'-end initiation codon of the discoloring enzyme modifier gene tpmD' of the triphenylmethane dye and adding a small label that can chelate 6 encoding amino acids of a diarsenic fluorescent dye before a 3'-end termination codon. The discoloring Shiva engineering bacterium with multiple functions of fluorescent tracing and discoloring can be used for environmental reparation and administration.

Description

Technical field: [0001] The invention belongs to the fields of biotechnology and environmental engineering, and in particular relates to a fluorescent tracer multifunctional decolorizing decolorizing Shewanella engineering bacterium and a construction method thereof. Background technique: [0002] The pollution of dyes and dye wastewater to the environment and the harm to human beings have been widely concerned by the world, and have become a global ecological environment problem. The control and elimination of this pollution has attracted great attention of scholars from all over the world. Practice has proved that the anaerobic-aerobic biological enhancement treatment system is the most economical and effective technology. The ways to achieve bioaugmentation technology include: ① Adding effective degradable microorganisms; ② Optimizing the nutrient supply of the existing treatment system; ③ Adding genetically engineered bacteria. [0003] At present, the most common meth...

AI Technical Summary

Problems solved by technology

The enzyme can catalyze the decolorization and degradation of various triphenylmethane dyes such as crystal violet, basic fuchsin, brilliant green and malachite green. It has structural specificity for substrates and can efficiently decolorize and degrade triphenylmethane dye strains , but due to the potential pathogenicity of this type of bacteria to fish and frogs, the application of this strain in environmental bioremediation is limited

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