Trichodrema spp. microbial agent and preparation method thereof

A spore inoculant and technology of spore fungus, applied in the field of Trichoderma spore inoculum and its preparation, can solve the problems of polluting the environment of edible fungus production areas, breeding of molds and pests, occupying land, etc., and achieve enhanced resistance to adverse environmental conditions, Effect of Prolonged Survival Period and Excellent Culture Substrate

Inactive Publication Date: 2009-10-28
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If these edible fungus chaffs are discarded or piled up randomly, it will not only waste precious resources, but also occupy land, cause mold and pests to breed, and pollute the environment in edible fungi producing areas

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The present embodiment adopts and is composed of 80% of cottonseed hulls, 15% of rapeseed cake, 2% of gypsum powder, 2% of superphosphate, and 1% of urea, the culture medium waste after the production of oyster mushrooms. Select rod-shaped oyster mushroom chaff with white hyphae, solid material, no mildew and rot, remove the plastic bag film outside the fungus bag, spread out the fungus chaff by drying in the sun, and dry the chaff after drying The water content is 10%-15%. The dried fungus chaff is crushed by a pulverizer, and the maximum diameter of the processed powdery fungus chaff is 0.20 cm.

[0018] Mix 8 parts by weight of edible fungus chaff after drying and pulverize with 2.035 parts of auxiliary raw materials, add water of 80% of the total weight of the mixed raw materials to the mixed raw materials, stir evenly, and adjust the mixed raw materials with 1M hydrochloric acid and lime powder The pH value is 6.0, put the adjusted mixed raw materials into a high-...

Embodiment 2

[0021] Present embodiment adopts and is composed of rice bran 35%, bagasse 35%, wheat bran 25%, urea 1%, cottonseed cake 2%, lime powder 1% and calcium carbonate 1% the medium waste material Flammulina velutipes produced after the production of Flammulina velutipes bran. Select Flammulina velutipes mushroom chaff with white hyphae, solid material, no mildew and rotten ingredients, remove the plastic bag film outside the fungus bag, spread out the fungus chaff by drying in the sun, and the water content of the fungus chaff after drying 10%-13%. The dried fungus chaff is crushed by a pulverizer, and the maximum diameter of the processed powdery fungus chaff is 0.3 cm.

[0022] Mix 8.5 parts by weight of edible fungus chaff after drying and pulverize with 3.05 parts of auxiliary raw materials, add water of 90% of the total weight of the mixed raw materials to the mixed raw materials, stir evenly, and adjust the mixed raw materials with 1M hydrochloric acid and lime powder The p...

Embodiment 3

[0025] The present embodiment adopts and is composed of 45% of corn stalk powder, 15% of chicken manure, 25% of wheat bran, 1% of urea, 2% of cottonseed cake, 1% of lime powder and 1% of calcium carbonate producing culture medium waste after Coprinus comatus Coprinus comatus chaff. Select the chaff of Coprinus comatus with white hyphae, solid material, no mildew and rot, remove the plastic bag film outside the fungus bag, spread it out and dry it in the sun to dry the chaff. After drying, the chaff contains The amount of water is 15%-18%. The dried fungus chaff is crushed by a pulverizer, and the maximum diameter of the processed powdery fungus chaff is 0.5 cm.

[0026] Mix 9 parts by weight of edible fungus chaff after drying and pulverize with 2.702 parts of auxiliary raw materials, add water of 85% of the total weight of the mixed raw materials to the mixed raw materials, stir evenly, and adjust the mixed raw materials with 1M hydrochloric acid and lime powder The pH valu...

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Abstract

The invention relates to a biological preparation, in particular to a Trichodrema spp. microbial agent and a preparation method thereof. The method comprises the following steps: the dried and crushed 8-9 edible fungus chaff according to parts by weight is mixed with 2-4 auxiliary material according to parts by weight, the acidity-alkalinity pH of the mixed raw material is adjusted to 5.0-6.5, then the Trichodrema spp. suspension with 5-10 percent of mass ratio with the mixed raw material is inoculated to the mixed raw material and cultured for 7-10 days at the temperature of 8-30 DEG C, and after the Trichodrema spp. is generated, the well cultured material is dried and crushed to obtain Trichodrema spp. microbial agent. The invention takes the treated edible fungus chaff as the raw material to produce the Trichodrema spp. microbial agent, fully utilizes the rest nutritious substance in the edible fungus chaff as well as the characteristics of loose and permeability of the fungus chaff, promotes the value in use of the fungus chaff of the edible fungus, and simultaneously reduces the production cost of the Trichodrema spp. microbial agent, the produced Trichodrema spp. is mainly chlamydospore, the multiplication capacity and the colonizing capacity of the Trichodrema spp. in the microbial agent are enhanced, the capability of resisting the unfavorable environmental conditions is also enhanced and the survival time of the Trichodrema spp. is prolonged.

Description

technical field [0001] The invention relates to a biological agent, in particular to a Trichoderma spore inoculum agent and a preparation method thereof. Background technique [0002] Trichoderma (Trichoderma spp.) belongs to Deuteromycotina, Hyphomycetes, Hyphosporales, and Myxospora. It is a class of fungi that are ubiquitous in nature. , The surface of the bulb can often be separated. Trichoderma is a class of antagonistic fungi with important biological control value, such as Trichoderma harzianum, Trichoderma hamatum and Trichoderma viride can control fungal diseases. Its mechanism of action has now been clarified, including the production of antibiotics, heavy parasitism, bacteriolysis, and competition. In addition, Trichoderma also stimulates plant growth and increases yield. Tests have shown that Trichoderma biological agents have good control effects on many crops, vegetables, flowers, tobacco damping-off, blight, silkworm, black shank, root rot, downy mildew, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/885
Inventor 张惠文阮晓东蔡颖慧张晓黎罗永平苏振成
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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